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01-02-2012 | Laboratory Investigation - Human/Animal Tissue

Regulation of histone acetylation by NDRG2 in glioma cells

Authors: Li Li, Xiaoqin Qin, Ming Shi, Rui Miao, Li Wang, Xinping Liu, Libo Yao, Yanchun Deng

Published in: Journal of Neuro-Oncology | Issue 3/2012

Abstract

NDRG2, a member of the N-Myc downstream-regulated gene family, was shown to be a putative tumor suppressor gene in glioblastoma and other cancers. Through a bioinformatic analysis, we found that NDRG2 protein contains an acyl carrier domain. In the current study, we therefore hypothesized that NDRG2 may play an important role in the regulation of histone acetylation. Treatment of U251 and U87 glioma cells with trichostatin A, an inhibitor of histone deacetylase, upregulated the expression of NDRG2 and acetylated forms of histones H3 and H4, reduced tumor cell viability and arrested the cell cycle at the G1/G0 phase. Overexpression of NDRG2 by transfecting glioma cells with adenovirus containing the NDRG2 gene upregulated the levels of acetylated forms of H3 and H4 whereas inhibition of NDRG2 expression by siRNA-mediated knockdown downregulated the level of histone acetylation. Furthermore, NDRG2 siRNA significantly reduced the level of histone acetylation induced by trichostatin A. Taken together, these data demonstrate that NDRG2 can regulate the level of histone acetylation to control glioma cell growth.

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Title: Regulation of histone acetylation by NDRG2 in glioma cells
Authors: Li Li
Xiaoqin Qin
Ming Shi
Rui Miao
Li Wang
Xinping Liu
Libo Yao
Yanchun Deng
Publication date: 01-02-2012
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 3/2012
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-011-0700-8

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Regulation of histone acetylation by NDRG2 in glioma cells

Abstract

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Abstract

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