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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 12/2014

01-12-2014 | Embryo Biology

Reduced uterine receptivity for mouse embryos developed from in-vitro matured oocytes

Authors: Yu-Hung Lin, Ching-Yen Tsai, Lee-Wen Huang, Kok-Min Seow, Jiann-Loung Hwang, Chii-Ruey Tzeng

Published in: Journal of Assisted Reproduction and Genetics | Issue 12/2014

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Abstract

Purpose

The outcomes of in-vitro maturation (IVM) are inferior compared to those of IVF. The purpose of the study was to compare the implantation rates of IVM- and in-vivo maturation (IVO)- derived embryos, and to evaluate their effects on uterine receptivity.

Methods

The IVM- and IVO- oocytes were obtained from female mice, fertilized and transferred to separate oviducts of the same pseudo-pregnant mice. After 5 days, the implanted blastocysts were dissected out of the uterine horns, and the uterine horns were analyzed for the expression of mRNAs encoding leukemia inhibitory factor, heparin-binding epidermal growth factor, insulin-like growth factor binding protein-4, progesterone receptor, and Hoxa-10.

Results

The maturation rate of the IVM- oocytes was 81.2 %. The fertilization rate of the IVM oocytes was lower than that of the IVO oocytes (50.5 % vs. 78.0 %, p = 0.038), as was their implantation rate (14.5 % vs. 74.7 %, p < 0.001). All 5 mRNAs examined were expressed at significantly lower levels in the uterine horns that received the IVM-derived embryos than in those that received the IVO-derived embryos.

Conclusions

The IVM-derived embryos are less competent in inducing expression of implantation-related mRNAs in the uterine horn.
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Metadata
Title
Reduced uterine receptivity for mouse embryos developed from in-vitro matured oocytes
Authors
Yu-Hung Lin
Ching-Yen Tsai
Lee-Wen Huang
Kok-Min Seow
Jiann-Loung Hwang
Chii-Ruey Tzeng
Publication date
01-12-2014
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 12/2014
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-014-0354-5

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