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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-05-2013 | Basic Science

Reconstruction of orbital defects by implantation of antigen-free bovine cancellous bone scaffold combined with bone marrow mesenchymal stem cells in rats

Authors: Jingjing Zhao, Chunbo Yang, Chang Su, Min Yu, Xiaomin Zhang, Shuo Huang, Gang Li, Meili Yu, Xiaorong Li

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 5/2013

Abstract

Background

Tissue-engineering approach can result in significant bone regeneration. We aimed to reconstruct the segmental orbital rim defects with antigen-free bovine cancellous bone (BCB) scaffolds combined with bone marrow mesenchymal stem cells (BMSCs) in rats.

Methods

BCB was prepared by degreasing, deproteinization and partly decalcification. BMSCs isolated from green fluorescent protein (GFP) transgenic rats were osteogenically induced and seeded onto BCB scaffolds to construct induced BMSCs/BCB composites. An 8-mm full-thickness defect on the rat inferior-orbit rim was established. Induced BMSCs/BCB composites cultured for 5 days were implanted into the orbital defects as the experimental group. Noninduced BMSCs/BCB group, BCB group and exclusive group were set. General condition, spiral CT, 3D orbital reconstruction, histological and histomorphometric analysis were performed after implantation.

Results

BCB presented reticular porous structure. GFP-BMSCs adhering to BCB appeared bright green fluorescence and grew vigorously. Infection and graft dislocation were not observed. In induced BMSCs/BCB group, CT and 3D reconstruction showed perfect orbital repair situation. Histological analysis indicated BCB was mostly biodegraded; newly formed bone and complete synostosis were observed. The percentage of newly formed bone was (57.12 ± 6.28) %. In contrast, more residual BCB, less newly formed bone and nonunion were observed in the noninduced BMSCs/BCB group. Slowly absorbed BCB enwrapped by fibrous connective tissue and a small amount of new bone occurred in BCB group. Fibrous connective tissue appeared in exclusive group.

Conclusions

Antigen-free bovine cancellous bone that retains natural bone porous structure and moderate mechanical strength with elimination of antigen is the ideal carrier for mesenchymal stem cells in vitro. BCB combined with BMSCs is a promising composite for tissue engineering, and can effectively reconstruct the orbit rim defects in rats.

Iatrou I, Theologie-Lygidakis N, Angelopoulos A (2001) Use of membrane and bone grafts in the reconstruction of orbit fractures. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91:281–286PubMedCrossRef

Heary RF, Schlenk RP, Sacchieri TA, Barone D, Brotea C (2002) Persistent iliac crest donor site pain: independent outcome assessment. Neurosurgery 50:510–517PubMed

Nishida J, Shimamura T (2008) Methods of reconstruction for bone defect after tumor excision: a review of alternatives. Med Sci Monit 14:RA107–RA113PubMed

Potter JK, Ellis E (2004) Biomaterials for reconstruction of the internal orbit. J Oral Maxillofac Surg 62:1280–1297PubMedCrossRef

Lee S, Maronian N, Most SP, Whipple ME, McCulloch TM, Stanley RB, Farwell DG (2005) Porous high-density polyethylene for orbital reconstruction. Arch Otolaryngol Head Neck Surg 131:446–450PubMedCrossRef

Wang ML, Tuli R, Manner PA, Sharkey PF, Hall DJ, Tuan RS (2003) Direct and indirect induction of apoptosis in human mesenchymal stem cells in response to titanium particles. J Orthop Res 21:697–707PubMedCrossRef

Kontio R, Suuronen R, Salonen O, Paukku P, Konttinen YT, Lindqvist C (2001) Int J Oral Maxillofac Surg 30:278–285PubMedCrossRef

Ceonzo K, Gaynor A, Shaffer L, Kojima K, Vacanti CA, Stahl GL (2006) Polyglycolic acid-induced inflammation: role of hydrolysis and resulting complement activation. Tissue Eng 12:301–308PubMedCrossRef

Liu Y, Ramanath HS, Wang DA (2008) Tendon tissue engineering using scaffold enhancing strategies. Trends Biotechnol 26:201–209PubMedCrossRef

10.

Sokolsky-Papkov M, Agashi K, Olaye A, Shakesheff K, Domb AJ (2007) Polymer carriers for drug delivery in tissue engineering. Adv Drug Deliv Rev 59:187–206PubMedCrossRef

11.

Yildirim M, Spiekermann H, Handt S, Edelhoff D (2001) Maxillary sinus augmentation with the xenograft Bio-Oss and autogenous intraoral bone for qualitative improvement of the implant site: a histologic and histomorphometric clinical study in humans. Int J Oral Maxillofac Implants 16:23–33PubMed

12.

Young C, Sandstedt P, Skoglund A (1999) A comparative study of anorganic xenogenic bone and autogenous bone implants for bone regeneration in rabbits. Int J Oral Maxillofac Implants 14:72–76PubMed

13.

Cui L, Liu B, Liu G, Zhang W, Cen L, Sun J, Yin S, Liu W, Cao Y (2007) Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model. Biomaterials 28:5477–5486PubMedCrossRef

14.

Kon E, Muraglia A, Corsi A, Bianco P, Marcacci M, Martin I, Boyde A, Ruspantini I, Chistolini P, Rocca M, Giardino R, Cancedda R, Quarto R (2000) Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in criticalsize defects of sheep long bones. J Biomed Mater Res 49:328–337PubMedCrossRef

15.

Song C, Li G (2011) CXCR4 and matrix metalloproteinase-2 are involved in mesenchymal stromal cell homing and engraftment to tumors. Cytotherapy 13:549–561PubMedCrossRef

16.

Chen X, McClurg A, Zhou GQ, McCaigue M, Armstrong MA, Li G (2007) Chondrogenic differentiation alters the immunosuppressive property of bone marrow-derived mesenchymal stem cells, and the effect is partially due to the upregulated expression of B7 molecules. Stem Cells 25(2):364–370PubMedCrossRef

17.

Ladage D, Brixius K, Steingen C, Mehlhorn U, Schwinger RH, Bloch W, Schmidt A (2007) Mesenchymal stem cells induce endothelial activation via paracine mechanisms. Endothelium 14:53–63PubMedCrossRef

18.

Worth A, Mucalo M, Horne G, Bruce W, Burbidge H (2005) The evaluation of processed cancellous bovine bone as a bone graft substitute. Clin Oral Implants Res 16:379–386PubMedCrossRef

19.

Kneser U, Stangenberg L, Ohnolz J, Buettner O, Stern-Straeter J, Möbest D, Horch RE, Stark GB, Schaefer DJ (2006) Evaluation of processed bovine cancellous bone matrix seeded with syngenic osteoblasts in a critical size calvarial defect rat model. J Cell Mol Med 10:695–707PubMedCrossRef

20.

Mushipe MT, Revell P, Shelton JC (2002) Cancellous bone repair using bovine trabecular bone matrix particles. Biomaterials 23:365–370PubMedCrossRef

21.

Khaled EG, Saleh M, Hindocha S, Griffin M, Khan WS, Khaled EG (2011) Tissue engineering for bone production-stem cells, gene therapy and scaffolds. Open Orthop J 5(Suppl 2):289–295PubMedCrossRef

22.

Maniatopoulos C, Sodek J, Melcher AH (1988) Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res 254:317–330PubMedCrossRef

23.

Zambuzzi WF, Oliveira RC, Pereira FL, Cestari TM, Taga R, Granjeiro JM (2006) Rat subcutaneous tissue response to macrogranular porous anorganic bovine bone graft. Braz Dent J 17:274–278PubMedCrossRef

24.

Han D, Li J, Guan X (2010) Ectopic osteogenesis of hBMP-2 gene-transduced human bone mesenchymal stem cells/BCB. Connect Tissue Res 51:274–281PubMedCrossRef

25.

He H, Cao J, Wang D, Gu B, Guo H, Liu H (2010) Gene-modified stem cells combined with rapid prototyping techniques: a novel strategy for periodontal regeneration. Stem Cell Rev 6:137–141PubMedCrossRef

26.

Schmitz JP, Hollinger JO (1986) The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat R 205:299–308

27.

Schmitz JP, Schwartz Z, Hollinger JO, Boyan BD (1990) Characterization of rat calvarial nonunion defects. Acta Anat (Basel) 138:185–192CrossRef

28.

Yoon E, Dhar S, Chun DE, Gharibjanian NA, Evans GR (2007) In vivo osteogenic potential of human adipose-derived stem cells/poly lactide-co-glycolic acid constructs for bone regeneration in a rat critical-sized calvarial defect model. Tissue Eng 13(3):619–627PubMedCrossRef

Title: Reconstruction of orbital defects by implantation of antigen-free bovine cancellous bone scaffold combined with bone marrow mesenchymal stem cells in rats
Authors: Jingjing Zhao
Chunbo Yang
Chang Su
Min Yu
Xiaomin Zhang
Shuo Huang
Gang Li
Meili Yu
Xiaorong Li
Publication date: 01-05-2013
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 5/2013
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-013-2300-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Reconstruction of orbital defects by implantation of antigen-free bovine cancellous bone scaffold combined with bone marrow mesenchymal stem cells in rats

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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