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Published in: International Journal of Legal Medicine 5/2005

01-09-2005 | Technical Note

Recommendations for animal DNA forensic and identity testing

Authors: Bruce Budowle, Paolo Garofano, Andreas Hellman, Melba Ketchum, Sree Kanthaswamy, Walther Parson, Wim van Haeringen, Steve Fain, Tom Broad

Published in: International Journal of Legal Medicine | Issue 5/2005

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Abstract

Genetic analysis in animals has been used for many applications, such as kinship analysis, for determining the sire of an offspring when a female has been exposed to multiple males, determining parentage when an animal switches offspring with another dam, extended lineage reconstruction, estimating inbreeding, identification in breed registries, and speciation. It now also is being used increasingly to characterize animal materials in forensic cases. As such, it is important to operate under a set of minimum guidelines that assures that all service providers have a template to follow for quality practices. None have been delineated for animal genetic identity testing. Based on the model for human DNA forensic analyses, a basic discussion of the issues and guidelines is provided for animal testing to include analytical practices, data evaluation, nomenclature, allele designation, statistics, validation, proficiency testing, lineage markers, casework files, and reporting. These should provide a basis for professional societies and/or working groups to establish more formalized recommendations.
Literature
1.
go back to reference D’Andrea F, Fridez F, Coquoz R (1998) Preliminary experiments on the transfer of animal hair during simulated criminal behaviour. J Forensic Sci 43(6):1257–1258 D’Andrea F, Fridez F, Coquoz R (1998) Preliminary experiments on the transfer of animal hair during simulated criminal behaviour. J Forensic Sci 43(6):1257–1258
2.
go back to reference Bartlett SE, Davidson WS (1992) FINS (Forensically Informative Nucleotide Sequencing): a procedure for identifying the animal origin of biological specimens. BioTechniques 12:82–92 Bartlett SE, Davidson WS (1992) FINS (Forensically Informative Nucleotide Sequencing): a procedure for identifying the animal origin of biological specimens. BioTechniques 12:82–92
3.
go back to reference Eichmann C, Berger B, Reinhold M, Lutz M, Parson W (2004) Canine-specific STR typing of saliva traces on dog bite wounds. Int J Legal Med 118:337–342 Eichmann C, Berger B, Reinhold M, Lutz M, Parson W (2004) Canine-specific STR typing of saliva traces on dog bite wounds. Int J Legal Med 118:337–342
4.
go back to reference Eichmann C, Berger B, Parson W (2004) A proposed nomenclature for 15 canine-specific polymorphic STR loci for forensic purposes. Int J Legal Med 118:249–266 Eichmann C, Berger B, Parson W (2004) A proposed nomenclature for 15 canine-specific polymorphic STR loci for forensic purposes. Int J Legal Med 118:249–266
5.
go back to reference Eichmann C, Berger B, Steinlechner M, Parson W (2005) Estimating the probability of identity in a random dog population using fifteen highly polymorphic canine STR markers. Forensic Sci Int (in press) Eichmann C, Berger B, Steinlechner M, Parson W (2005) Estimating the probability of identity in a random dog population using fifteen highly polymorphic canine STR markers. Forensic Sci Int (in press)
6.
go back to reference Menotti-Raymond MA, David VA, O’Brien SJ (1997) Pet cat hair implicates murder suspect. Nature 386:774 Menotti-Raymond MA, David VA, O’Brien SJ (1997) Pet cat hair implicates murder suspect. Nature 386:774
7.
go back to reference Menotti-Raymond MA, O’Brien SJ (1995) Evolutionary conservation of ten microsatellite loci in four species of Felidae. J Heredity 86(4):319–322 Menotti-Raymond MA, O’Brien SJ (1995) Evolutionary conservation of ten microsatellite loci in four species of Felidae. J Heredity 86(4):319–322
8.
go back to reference Moore JE (1988) A key for the identification of animal hairs. J Forensic Sci 28:335–339 Moore JE (1988) A key for the identification of animal hairs. J Forensic Sci 28:335–339
9.
go back to reference Pádár Z, Egyed B, Kontadakis K, Füredi S, Woller J, Zöldág L, Fekete S (2002) Canine STR analyses in forensic practice—observation of a possible mutation in a dog hair. Int J Legal Med 116:286–288 Pádár Z, Egyed B, Kontadakis K, Füredi S, Woller J, Zöldág L, Fekete S (2002) Canine STR analyses in forensic practice—observation of a possible mutation in a dog hair. Int J Legal Med 116:286–288
10.
go back to reference Paetkau D, Calvert W, Stirling I, Strobeck C (1995) Microsatellite analysis of population structure in Canadian polar bears. Mol Ecol 4:347–354 Paetkau D, Calvert W, Stirling I, Strobeck C (1995) Microsatellite analysis of population structure in Canadian polar bears. Mol Ecol 4:347–354
11.
go back to reference Schneider PM, Seo Y, Rittner C (1999) Forensic mtDNA hair analysis excludes a dog from having caused a traffic accident. Int J Legal Med 112(5):315–316 Schneider PM, Seo Y, Rittner C (1999) Forensic mtDNA hair analysis excludes a dog from having caused a traffic accident. Int J Legal Med 112(5):315–316
12.
go back to reference Savolainen P, Rosén B, Holmberg A, Leitner T, Uhlén M, Lundeberg J (1997) Sequence analysis of domestic dog mitochondrial DNA for forensic use. J Forensic Sci 42:593–600 Savolainen P, Rosén B, Holmberg A, Leitner T, Uhlén M, Lundeberg J (1997) Sequence analysis of domestic dog mitochondrial DNA for forensic use. J Forensic Sci 42:593–600
13.
go back to reference Savolainen P, Lundeberg J (1999) Forensic evidence based on mtDNA from dog and wolf hairs. J Forensic Sci 44:77–81 Savolainen P, Lundeberg J (1999) Forensic evidence based on mtDNA from dog and wolf hairs. J Forensic Sci 44:77–81
14.
go back to reference Shutler GG, Gagnon P, Verret G, Kalyn H, Korkosh S, Johnston E, Halverson J (1999) Removal of a PCR inhibitor and resolution of DNA STR types in mixed human-canine stains from a five year old case. J Forensic Sci 44(3):623–626 Shutler GG, Gagnon P, Verret G, Kalyn H, Korkosh S, Johnston E, Halverson J (1999) Removal of a PCR inhibitor and resolution of DNA STR types in mixed human-canine stains from a five year old case. J Forensic Sci 44(3):623–626
15.
go back to reference Wetton JH, Higgs JE, Spriggs AC, Roney CA, Tsang CSF, Foster AP (2003) Mitochondrial profiling of dog hairs. Forensic Sci Int 133:235–241 Wetton JH, Higgs JE, Spriggs AC, Roney CA, Tsang CSF, Foster AP (2003) Mitochondrial profiling of dog hairs. Forensic Sci Int 133:235–241
17.
go back to reference Bar W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B (1997) DNA recommendations. Further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. International society for forensic haemogenetics. Int J Legal Med 110(4):175–176 Bar W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B (1997) DNA recommendations. Further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. International society for forensic haemogenetics. Int J Legal Med 110(4):175–176
18.
go back to reference Bar W, Brinkmann B, Budowle B, Carracedo A, Gill P, Holland M, Lincoln PJ, Mayr W, Morling N, Olaisen B, Schneider PM, Tully G, Wilson M (2000) DNA Commission of the international society for forensic genetics: guidelines for mitochondrial DNA typing. Int J Legal Med 113(4):193–196; and Forensic Sci Int 110(2):79–85 Bar W, Brinkmann B, Budowle B, Carracedo A, Gill P, Holland M, Lincoln PJ, Mayr W, Morling N, Olaisen B, Schneider PM, Tully G, Wilson M (2000) DNA Commission of the international society for forensic genetics: guidelines for mitochondrial DNA typing. Int J Legal Med 113(4):193–196; and Forensic Sci Int 110(2):79–85
19.
go back to reference Budowle B, Smith JA, Moretti T, DiZinno J (2000) Quality assurance standards for forensic DNA testing laboratories. In: DNA typing protocols: molecular biology and forensic analysis, biotechniques books, bioforensic sciences series. Eaton Publishing, Natick, MA, pp 228–243 Budowle B, Smith JA, Moretti T, DiZinno J (2000) Quality assurance standards for forensic DNA testing laboratories. In: DNA typing protocols: molecular biology and forensic analysis, biotechniques books, bioforensic sciences series. Eaton Publishing, Natick, MA, pp 228–243
20.
go back to reference Gill P, Brenner C, Brinkmann B, Budowle B, Carracedo A, Jobling MA, de Knijff P, Kayser M, Krawczak M, Mayr WR, Morling N, Olaisen B, Pascali V, Prinz M, Roewer L, Schneider PM, Sajantila A, Tyler-Smith C (2001) DNA commission of the International society of forensic genetics: recommendations on forensic analysis using Y-chromosome STRs. Int J Legal Med 114(6):305–309 Gill P, Brenner C, Brinkmann B, Budowle B, Carracedo A, Jobling MA, de Knijff P, Kayser M, Krawczak M, Mayr WR, Morling N, Olaisen B, Pascali V, Prinz M, Roewer L, Schneider PM, Sajantila A, Tyler-Smith C (2001) DNA commission of the International society of forensic genetics: recommendations on forensic analysis using Y-chromosome STRs. Int J Legal Med 114(6):305–309
21.
go back to reference Jobling MA, Gill P (2004) Encoded evidence: DNA in forensic analysis. Nat Rev Genet 5(10):739–751 Jobling MA, Gill P (2004) Encoded evidence: DNA in forensic analysis. Nat Rev Genet 5(10):739–751
22.
go back to reference Morling N, Allen R, Carracedo A, Geada H, Guidet F, Hallenberg C, Martin W, Mayr WR, Olaisen B, Pascali V, Schneider PM (2003) Recommendations on genetic investigations in paternity cases. Paternity testing commission of the international society of forensic genetics. Int J Legal Med 117(1):51–61 Morling N, Allen R, Carracedo A, Geada H, Guidet F, Hallenberg C, Martin W, Mayr WR, Olaisen B, Pascali V, Schneider PM (2003) Recommendations on genetic investigations in paternity cases. Paternity testing commission of the international society of forensic genetics. Int J Legal Med 117(1):51–61
23.
go back to reference Lazaruk K, Walsh PS, Oaks F, Gilbert D, Rosenblum BB, Menchen S, Scheibler D, Wenz HM, Holt C, Wallin J (1998) Genotyping of forensic short tandem repeat (STR) systems based on sizing precision in a capillary electrophoresis instrument. Electrophoresis 19(1):86–93 Lazaruk K, Walsh PS, Oaks F, Gilbert D, Rosenblum BB, Menchen S, Scheibler D, Wenz HM, Holt C, Wallin J (1998) Genotyping of forensic short tandem repeat (STR) systems based on sizing precision in a capillary electrophoresis instrument. Electrophoresis 19(1):86–93
25.
go back to reference Evett IW, Weir BS (1998) Interpreting DNA evidence: statistical genetics for forensic scientists. Sinauer, Sunderland Evett IW, Weir BS (1998) Interpreting DNA evidence: statistical genetics for forensic scientists. Sinauer, Sunderland
26.
go back to reference Waits LP, Luikart G, Taberlet P (2001) Estimating the probability of identity among genotypes in natural populations: cautions and guidelines. Mol Ecol 10(1):249–256 Waits LP, Luikart G, Taberlet P (2001) Estimating the probability of identity among genotypes in natural populations: cautions and guidelines. Mol Ecol 10(1):249–256
27.
go back to reference Brinkmann B, Klintschar M, Neuhuber F, Huhne J, Rolf B (1998) Mutation rate in human microsatellites: influence of the structure and length of the tandem repeat. Am J Hum Genet 62:1408–1415 Brinkmann B, Klintschar M, Neuhuber F, Huhne J, Rolf B (1998) Mutation rate in human microsatellites: influence of the structure and length of the tandem repeat. Am J Hum Genet 62:1408–1415
28.
go back to reference Sajantila A, Lukka M, Syvanen AC (1999) Experimentally observed germline mutations at human micro- and minisatellite loci. Eur J Hum Genet 7:263–266 Sajantila A, Lukka M, Syvanen AC (1999) Experimentally observed germline mutations at human micro- and minisatellite loci. Eur J Hum Genet 7:263–266
29.
go back to reference Bravi CM, Liron JP, Mirol PM, Ripoli MV, Peral-Garcia P, Giovambattista G (2004) A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene. Legal Med 6:246–251 Bravi CM, Liron JP, Mirol PM, Ripoli MV, Peral-Garcia P, Giovambattista G (2004) A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene. Legal Med 6:246–251
30.
go back to reference Linacre A, Lee JC (2004) Species determination: the role and use of the cytochrome B gene. Methods Mol Biol 297:45–52 Linacre A, Lee JC (2004) Species determination: the role and use of the cytochrome B gene. Methods Mol Biol 297:45–52
31.
go back to reference Parson W, Pegoraro K, Niederstatter H, Foger M, Steinlechner M (2000) Species identification by means of the cytochrome b gene. Int J Legal Med 114:23–28 Parson W, Pegoraro K, Niederstatter H, Foger M, Steinlechner M (2000) Species identification by means of the cytochrome b gene. Int J Legal Med 114:23–28
32.
go back to reference Pepe T, Trotta M, di Marco I, Cennamo P, Anastasio A, Cortesi ML (2005) Mitochondrial cytochrome b DNA sequence variations: an approach to fish species identification in processed fish products. J Food Prot 68:421–425 Pepe T, Trotta M, di Marco I, Cennamo P, Anastasio A, Cortesi ML (2005) Mitochondrial cytochrome b DNA sequence variations: an approach to fish species identification in processed fish products. J Food Prot 68:421–425
33.
go back to reference Perez J, Garcia-Vazquez E (2004) Genetic identification of nine hake species for detection of commercial fraud. J Food Prot 67:2792–2796 Perez J, Garcia-Vazquez E (2004) Genetic identification of nine hake species for detection of commercial fraud. J Food Prot 67:2792–2796
34.
go back to reference Rajapaksha WR, Thilakaratne ID, Chandrasiri AD, Niroshan TD (2002) Development of PCR assay for differentiation of some important wild animal meat of Sri Lanka. J Vet Med B Infect Dis Vet Public Health 49:322–324 Rajapaksha WR, Thilakaratne ID, Chandrasiri AD, Niroshan TD (2002) Development of PCR assay for differentiation of some important wild animal meat of Sri Lanka. J Vet Med B Infect Dis Vet Public Health 49:322–324
35.
go back to reference Subramanian S, Karthik T, Vijayaraaghavan NN (2005) Single nucleotide polymorphism for animal fibre identification. J Biotechnol 116:153–158 Subramanian S, Karthik T, Vijayaraaghavan NN (2005) Single nucleotide polymorphism for animal fibre identification. J Biotechnol 116:153–158
36.
go back to reference Wan QH, Fang SG (2003) Application of species-specific polymerase chain reaction in the forensic identification of tiger species. Forensic Sci Int 131:75–78 Wan QH, Fang SG (2003) Application of species-specific polymerase chain reaction in the forensic identification of tiger species. Forensic Sci Int 131:75–78
37.
go back to reference Balitzki-Korte B, Anslinger K, Bartsch C, Rolf B (2005) Development of Y-chromosomal microsatellite markers for nonhuman primates. Int J Legal Med (in press) Balitzki-Korte B, Anslinger K, Bartsch C, Rolf B (2005) Development of Y-chromosomal microsatellite markers for nonhuman primates. Int J Legal Med (in press)
38.
go back to reference Erler A, Stoneking M, Kayser M (2004) Development of Y-chromosomal microsatellite markers for nonhuman primates. Mol Ecol 13:2921–2930 Erler A, Stoneking M, Kayser M (2004) Development of Y-chromosomal microsatellite markers for nonhuman primates. Mol Ecol 13:2921–2930
39.
go back to reference Olivier M, Breen M, Binns M, Lust G (1999) Localization and characterization of nucleotide sequences from the canine Y chromosome. Chromosome Res 7:223–233 Olivier M, Breen M, Binns M, Lust G (1999) Localization and characterization of nucleotide sequences from the canine Y chromosome. Chromosome Res 7:223–233
40.
go back to reference Sundqvist A-K, Ellegren H, Olivier M, Vila C (2001) Y chromosome haplotyping in Scandinavian wolves (Canis lupus) based on microsatellite markers. Mol Ecol 10:1959–1966 Sundqvist A-K, Ellegren H, Olivier M, Vila C (2001) Y chromosome haplotyping in Scandinavian wolves (Canis lupus) based on microsatellite markers. Mol Ecol 10:1959–1966
41.
go back to reference Van Hooft WF, Groen AF, Prins HT (2002) Phylogeography of the African buffalo based on mitochondrial and Y-chromosomal loci: Pleistocene origin and population expansion of the Cape buffalo subspecies. Mol Ecol 11:267–279 Van Hooft WF, Groen AF, Prins HT (2002) Phylogeography of the African buffalo based on mitochondrial and Y-chromosomal loci: Pleistocene origin and population expansion of the Cape buffalo subspecies. Mol Ecol 11:267–279
42.
go back to reference Verkaar ELC, Vervaecke H, Roden C, Mendoza LR, Barwegen MW, Susilawati T, Nijman IJ, Lenstra JA (2003) Paternally inherited markers in bovine hybrid populations. Heredity 91:565–569 Verkaar ELC, Vervaecke H, Roden C, Mendoza LR, Barwegen MW, Susilawati T, Nijman IJ, Lenstra JA (2003) Paternally inherited markers in bovine hybrid populations. Heredity 91:565–569
43.
go back to reference Verkaar ELC, Nijman IJ, Beeke M, Hanekamp E, Lenstra JA (2004) Maternal and paternal lineages in cross-breeding bovine species. Has Wisent a hybrid origin? Mol Biol Evol 21:1165–1170 Verkaar ELC, Nijman IJ, Beeke M, Hanekamp E, Lenstra JA (2004) Maternal and paternal lineages in cross-breeding bovine species. Has Wisent a hybrid origin? Mol Biol Evol 21:1165–1170
44.
go back to reference Wallner B, Brem G, Muller M, Achmann R (2003) Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalski and Equus caballus. Anim Genet 34:453–456 Wallner B, Brem G, Muller M, Achmann R (2003) Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalski and Equus caballus. Anim Genet 34:453–456
Metadata
Title
Recommendations for animal DNA forensic and identity testing
Authors
Bruce Budowle
Paolo Garofano
Andreas Hellman
Melba Ketchum
Sree Kanthaswamy
Walther Parson
Wim van Haeringen
Steve Fain
Tom Broad
Publication date
01-09-2005
Publisher
Springer-Verlag
Published in
International Journal of Legal Medicine / Issue 5/2005
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-005-0545-9

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