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Virology Journal
Published in: Virology Journal 1/2011

Open Access 01-12-2011 | Research

Recombinant luciferase-expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors

Authors: Ran He, Gordon Sandford, Gary S Hayward, William H Burns, Gary H Posner, Michael Forman, Ravit Arav-Boger

Published in: Virology Journal | Issue 1/2011

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Abstract

Recombinant Towne CMV expressing luciferase under the control of CMV-DNA polymerase (POL) or the late pp28 (UL99) promoters were evaluated for potential application in high-throughput screening of anti-viral compounds. POL-and pp28-luciferase displayed maximal expression 48 and 72 hours post infection, respectively. The pp28-luciferase virus achieved a wider dynamic range of luciferase expression (6-7 logs) and was selected for testing of inhibition by five anti-viral compounds. Luciferase expression highly correlated with plaque reduction and real-time PCR. The pp28-luciferase reporter system is rapid, reproducible, and highly sensitive. It may be applied to screening of novel anti-CMV compounds.
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Metadata
Title
Recombinant luciferase-expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors
Authors
Ran He
Gordon Sandford
Gary S Hayward
William H Burns
Gary H Posner
Michael Forman
Ravit Arav-Boger
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-8-40

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