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01-05-2015 | Research Article

RBPJ inhibition impairs the growth of lung cancer

Authors: Qun Lv, Ronglin Shen, Jianjun Wang

Published in: Tumor Biology | Issue 5/2015

Abstract

The exact effects of the modulation of Notch signaling pathway on cell growth have been shown to depend on tumor cell type. Recombination signal-binding protein Jκ (RBPJ) is a key transcription factor downstream of receptor activation in Notch signaling pathway. Here, we evaluated the effects of RBPJ inhibition on the growth of lung cancer cells. We found that a short hairpin interfering RNA (shRNA) for RBPJ efficiently inhibited RBPJ expression in lung cancer cells, resulting in a significant decrease in the cell growth. Further analyses showed that RBPJ inhibition altered the levels of its downstream targets, including p21, p27, CDK2, Hes1, Bcl-2, and SKP2, to prevent the cells from growing. Our data thus suggest that shRNA intervention of RBPJ expression could be a promising therapeutic approach for treating human lung cancer.

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Title: RBPJ inhibition impairs the growth of lung cancer
Authors: Qun Lv
Ronglin Shen
Jianjun Wang
Publication date: 01-05-2015
Publisher: Springer Netherlands
Published in: Tumor Biology / Issue 5/2015
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-014-3015-5

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