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Open Access 01-12-2011 | Methodology

Rapid detection of anti-Vaccinia virus neutralizing antibodies

Authors: Marit Kramski, Anna Drozd, Gregor F Lichtfuss, Piotr W Dabrowski, Heinz Ellerbrok

Published in: Virology Journal | Issue 1/2011

Abstract

Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was developed to determine neutralizing antibody titers within twelve hours. The new assay measures viral mRNA transcription as a marker for actively replicating virus after incomplete neutralization using real-time PCR.

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Title: Rapid detection of anti-Vaccinia virus neutralizing antibodies
Authors: Marit Kramski
Anna Drozd
Gregor F Lichtfuss
Piotr W Dabrowski
Heinz Ellerbrok
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-8-139

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