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Open Access 01-01-2017 | Clinical Study

Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study

Authors: Pedram Tabatabaei, Eward Visse, Per Bergström, Thomas Brännström, Peter Siesjö, A. Tommy Bergenheim

Published in: Journal of Neuro-Oncology | Issue 1/2017

Abstract

The knowledge of response to radiation in the immuno-microenvironment of high grade gliomas is sparse. In vitro results have indicated an inflammatory response of myeloid cells after irradiation. Therefore, microdialysis was used to verify whether this is operative in tumor tissue and brain adjacent to tumor (BAT) after clinical radiotherapy of patients with high grade glioma. Stereotactic biopsies and implantation of microdialysis catheters in tumor tissue and BAT were performed in eleven patients with high-grade glioma. The patients were given daily radiation fractions of 2–3.4 Gy. Microdialysis samples were collected before radiotherapy and during the first five days of radiation. Cytokines, glucose metabolites, glutamate and glycerol were analyzed. Immunohistochemistry was performed to detect macrophages (CD68) and monocytes (CD163) as well as IL-6, IL-8 and MCP-1. A significant increase of IL-8, MCP-1 and MIP-1a were detected in tumor tissue already after the first dose of radiation and increased further during 5 days of radiation. IL-6 did also increase but after five fractions of radiation. In BAT, the cytokine response was modest with significant increase of IL-8 after third dose of radiation. We found a positive correlation between baseline IL-8 and IL-6 microdialysis levels in tumor tissue and survival. Glucose metabolites or glycerol and glutamate did not change during radiation. In all tumors staining for macrophages was demonstrated. IL-6 was found in viable tumor cells while MCP-1 was demonstrated in macrophages or tumor matrix. Our findings suggest that radiation induces a rapid enhancement of the prevailing inflammation in high-grade glioma tissue. The microdialysis technique is feasible for this type of study and could be used to monitor metabolic changes after different interventions.

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Title: Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study
Authors: Pedram Tabatabaei
Eward Visse
Per Bergström
Thomas Brännström
Peter Siesjö
A. Tommy Bergenheim
Publication date: 01-01-2017
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 1/2017
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-016-2271-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study

Abstract

Keynote webinar | Spotlight on medication adherence

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Abstract

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