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Molecular Imaging and Biology

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01-08-2015 | Research Article

Radiation Dosimetry Study of [⁸⁹Zr]rituximab Tracer for Clinical Translation of B cell NHL Imaging using Positron Emission Tomography

Authors: Arutselvan Natarajan, Sanjiv Sam Gambhir

Published in: Molecular Imaging and Biology | Issue 4/2015

Abstract

Purpose

We evaluated the dosimetry of [⁸⁹Zr]rituximab, an anti-CD20 immunoPET tracer to image B cell non-Hodgkin’s lymphoma (NHL) using a humanized transgenic mouse model that expresses human CD20 transgenic mice (huCD20TM).

Procedures

Rituximab was conjugated to desferrioxamine (Df) for radiolabeling of Zirconium-89. [⁸⁹Zr]rituximab (2.8 ± 0.2 MBq) was tail vein-injected into huCD20T mice. Positron emission tomography (PET)/CT imaging was performed on the two groups of mice (blocking = 2 mg/kg pre-dose of rituximab and non-blocking; n = 5) at eight time points (1, 4, 24, 48, 72, 96, 120, and 168 h) post injection.

Results

The novel [⁸⁹Zr]rituximab PET tracer had good immunoreactivity, was stable in human serum, and was able to specifically target human CD20 in mice. The human equivalents of highest dose (mean ± SD) organs with and without pre-dose are liver (345 ± 284 μSv/MBq) and spleen (1165 ± 149 μSv/MBq), respectively.

Conclusions

Dosimetry of the human patient whole-body dose was found to be 145 MBq per annum, and the patient dose-limiting organ will be the liver (with rituximab pre-dose blocking) and spleen for non-blocking. The [⁸⁹Zr]rituximab (t½ = 78.4 h) imaging of B cell NHL patients could permit the observation of targeting lesions in NHL patients over an extended period due to longer half-life as compared to the [⁶⁴Cu] rituximab (t½ = 12.7 h).

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Title: Radiation Dosimetry Study of [89Zr]rituximab Tracer for Clinical Translation of B cell NHL Imaging using Positron Emission Tomography
Authors: Arutselvan Natarajan
Sanjiv Sam Gambhir
Publication date: 01-08-2015
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 4/2015
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-014-0810-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Radiation Dosimetry Study of [⁸⁹Zr]rituximab Tracer for Clinical Translation of B cell NHL Imaging using Positron Emission Tomography

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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