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BMC Complementary Medicine and Therapies

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Open Access 01-12-2021 | Research

R. vesicarius L. exerts nephroprotective effect against cisplatin-induced oxidative stress

Authors: Md. Mahmudul Hasan, Most. Sayla Tasmin, Ahmed M. El-Shehawi, Mona M. Elseehy, Md. Abu Reza, Ariful Haque

Published in: BMC Complementary Medicine and Therapies | Issue 1/2021

Abstract

Background

Cisplatin is an outstanding anticancer drug, but its use has been decreased remarkably due to sever nephrotoxicity. R. vesicarius L. is a leafy vegetable that is evident with anti-angeogenic, anti-inflammatory, anti-proliferative, hepatoprotective, and nephroprotective potential. Therefore, this study was designed to inspect its methanol extract (RVE) for possible nephroprotective effect.

Methods

Primarily, in vitro antioxidant activity of RVE was confirmed based on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging aptitude. Thereafter, Swiss Albino male mice were treated with cisplatin (2.5 mg/kg) for 5 successive days to induce nephrotoxicity. Recovery from nephrotoxicity was scrutinized by treating the animals with RVE (25, 50, and 100 mg/kg) intraperitoneally (i.p.) for the next 5 consecutive days. After completion of treatment, mice were sacrificed and kidneys were collected. Part of it was homogenized in sodium phosphate buffer for evaluating malondialdehyde (MDA) level, another part was used to evaluate gene (NQO1, p53, and Bcl-2) expression. Moreover, the hydrogen peroxide (H₂O₂) neutralizing capacity of RVE was evaluated in HK-2 cells in vitro. Finally, bioactive phytochemicals in RVE were determined using gas chromatography–mass spectrometry (GC-MS).

Results

RVE showed in vitro antioxidant activity in a dose-dependent fashion with 37.39 ± 1.89 μg/mL IC₅₀ value. Treatment with RVE remarkably (p < 0.05) decreased MDA content in kidney tissue. Besides, the expression of NQO, p53, and Bcl-2 genes was significantly (p < 0.05) mitigated in a dose-dependent manner due to the administration of RVE. RVE significantly (p < 0.05) reversed the H₂O₂ level in HK-2 cells to almost normal. From GC-MS, ten compounds including three known antioxidants “4H-Pyran-4-one, 2, 3-dihydro-3,5-dihydroxy-6-methyl-”, “Hexadecanoic acid”, and “Squalene” were detected. The extract was rich with an alkaloid “13-Docosenamide”.

Conclusion

Overall, RVE possesses a protective effect against cisplatin-induced kidney damage.

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Title: R. vesicarius L. exerts nephroprotective effect against cisplatin-induced oxidative stress
Authors: Md. Mahmudul Hasan
Most. Sayla Tasmin
Ahmed M. El-Shehawi
Mona M. Elseehy
Md. Abu Reza
Ariful Haque
Publication date: 01-12-2021
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2021
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-021-03398-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

R. vesicarius L. exerts nephroprotective effect against cisplatin-induced oxidative stress

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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