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International Journal of Colorectal Disease

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01-10-2011 | Original Article

Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients

Authors: Reza Raeisossadati, Moein Farshchian, Azita Ganji, Alieza Tavassoli, Arash Velayati, Ezzat Dadkhah, Somaye Chavoshi, Mostafa Mehrabi Bahar, Bahram Memar, Mohammad Taghi Rajabi Mashhadi, Hossein Naseh, Mohammad Mahdi Forghanifard, Meysam Moghbeli, Omeed Moaven, Mohammad Reza Abbaszadegan

Published in: International Journal of Colorectal Disease | Issue 10/2011

Abstract

Background

Colorectal cancer (CRC) remains the third most common cancer in the world. Approximately in 50 percent of patients, metastatic disease is a major cause of death. Therefore, early diagnosis of CRC is crucial for a successful outcome. For the detection of circulating cancer cells, this study applied a sensitive method that employed specific tumor markers for early detection.

Methods

A total of 80 blood samples from 40 CRC patients and 40 age-matched healthy controls were collected for the study. The circulating mRNA levels of two CRC tumor markers, tumor endothelial marker 8 (TEM-8) and carcinoembryogenic antigen (CEA) were evaluated using an absolute quantitative real-time PCR assay in a Stratagene Mx-3000P real-time PCR system. GAPDH was used as the endogenous control.

Results

TEM-8 and CEA were primarily detected more in the CRC patients rather than in the controls: 22/40 vs 9/40, p=0.009 and 30/40 vs 11/40, p=0.00054, respectively. In the CRC patients, the mRNA level of these markers was significantly higher in comparison to the normal controls (p=0.018 and 0.01). The overall sensitivity of this panel was 65% with a specificity of 75%. Statistical analysis for demographic variants did not reach significant values.

Conclusions

TEM-8 and CEA markers were detected more frequently and in significantly higher levels in the blood samples of patients compared with samples from age-matched healthy controls. The copy number of CEA and TEM-8 mRNA, as detected by a real-time quantitative PCR, appears to be a promising marker for evaluating the risk of tumor spread.

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Title: Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients
Authors: Reza Raeisossadati
Moein Farshchian
Azita Ganji
Alieza Tavassoli
Arash Velayati
Ezzat Dadkhah
Somaye Chavoshi
Mostafa Mehrabi Bahar
Bahram Memar
Mohammad Taghi Rajabi Mashhadi
Hossein Naseh
Mohammad Mahdi Forghanifard
Meysam Moghbeli
Omeed Moaven
Mohammad Reza Abbaszadegan
Publication date: 01-10-2011
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 10/2011
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-011-1230-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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