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Journal of the Association for Research in Otolaryngology
Published in: Journal of the Association for Research in Otolaryngology 2/2017

01-04-2017 | Research Article

Quantitative Analysis of Aquaporin Expression Levels during the Development and Maturation of the Inner Ear

Authors: Takushi Miyoshi, Taro Yamaguchi, Kiyokazu Ogita, Yasuko Tanaka, Ken-ichi Ishibashi, Hiroaki Ito, Taisuke Kobayashi, Takayuki Nakagawa, Juichi Ito, Koichi Omori, Norio Yamamoto

Published in: Journal of the Association for Research in Otolaryngology | Issue 2/2017

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Abstract

Aquaporins (AQPs) are a family of small membrane proteins that transport water molecules across the plasma membrane along the osmotic gradient. Mammals express 13 subtypes of AQPs, including the recently reported “subcellular AQPs”, AQP11 and 12. Each organ expresses specific subsets of AQP subtypes, and in the inner ear, AQPs are essential for the establishment and maintenance of two distinct fluids, endolymph and perilymph. To evaluate the contribution of AQPs during the establishment of inner ear function, we used quantitative reverse transcription polymerase chain reaction to quantify the expression levels of all known AQPs during the entire development and maturation of the inner ear. Using systematic and longitudinal quantification, we found that AQP11 was majorly and constantly expressed in the inner ear, and that the expression levels of several AQPs follow characteristic longitudinal patterns: increasing (Aqp0, 1, and 9), decreasing (Aqp6, 8, and 12), and peak of expression on E18 (Aqp2, 5, and 7). In particular, the expression level of Aqp9 increased by 70-fold during P3–P21. We also performed in situ hybridization of Aqp11, and determined the unique localization of Aqp11 in the outer hair cells. Immunohistochemistry of AQP9 revealed its localization in the supporting cells inside the organ of Corti, and in the root cells. The emergence of AQP9 expression in these cells was during P3–P21, which was coincident with the marked increase of its expression level. Combining these quantification and localization data, we discuss the possible contributions of these AQPs to inner ear function.
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Metadata
Title
Quantitative Analysis of Aquaporin Expression Levels during the Development and Maturation of the Inner Ear
Authors
Takushi Miyoshi
Taro Yamaguchi
Kiyokazu Ogita
Yasuko Tanaka
Ken-ichi Ishibashi
Hiroaki Ito
Taisuke Kobayashi
Takayuki Nakagawa
Juichi Ito
Koichi Omori
Norio Yamamoto
Publication date
01-04-2017
Publisher
Springer US
Published in
Journal of the Association for Research in Otolaryngology / Issue 2/2017
Print ISSN: 1525-3961
Electronic ISSN: 1438-7573
DOI
https://doi.org/10.1007/s10162-016-0607-3

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