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European Journal of Nuclear Medicine and Molecular Imaging

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01-08-2006 | Molecular imaging

Quantitation of cardiac sympathetic innervation in rabbits using ¹¹C-hydroxyephedrine PET: relation to ¹²³I-MIBG uptake

Authors: Yusuke Nomura, Ichiro Matsunari, Hiroyuki Takamatsu, Yoshihiro Murakami, Takahiro Matsuya, Junichi Taki, Kenichi Nakajima, Stephan G. Nekolla, Wei-Ping Chen, Kouji Kajinami

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 8/2006

Abstract

Purpose

Although ¹¹C-hydroxyephedrine (¹¹C-HED) PET is used to map cardiac sympathetic innervation, no studies have shown the feasibility of quantitation of ¹¹C-HED PET in small- to medium-sized animals. Furthermore, its relation to ¹²³I-MIBG uptake, the most widely used sympathetic nervous tracer, is unknown. The aims of this study were to establish in vivo sympathetic nerve imaging in rabbits using ¹¹C-HED PET, and to compare the retention of ¹¹C-HED with that of ¹²³I-MIBG.

Methods

Twelve rabbits were assigned to three groups; control (n=4), chemical denervation by 6-hydroxydopamine (6-OHDA) (n=4) and reserpine treated to inhibit vesicular uptake (n=4). After simultaneous injection of ¹¹C-HED and ¹²³I-MIBG, all animals underwent dynamic ¹¹C-HED PET for 40 min with arterial blood sampling. The ¹¹C-HED retention fraction and normalised ¹¹C-HED activity measured by tissue sampling were compared with those measured by PET.

Results

Both the ¹¹C-HED retention fraction and the normalised ¹¹C-HED activity measured by PET correlated closely with those measured by tissue sampling (R=0.96027, p<0.001 and R=0.97282, p<0.001, respectively). Inhibition study by 6-OHDA resulted in a significant reduction in retention (90%) for both ¹¹C-HED and ¹²³I-MIBG. Reserpine pretreatment reduced ¹¹C-HED retention by 50%, but did not reduce ¹²³I-MIBG retention at 40 min after injection.

Conclusion

Non-invasive quantitation of cardiac sympathetic innervation using ¹¹C-HED PET is feasible and gives reliable estimates of cardiac sympathetic innervation in rabbits. Additionally, although both ¹¹C-HED and ¹²³I-MIBG are specific for sympathetic neurons, ¹¹C-HED may be more specific for intravesicular uptake than ¹²³I-MIBG in some situations, such as that seen in reserpine pretreatment.

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Title: Quantitation of cardiac sympathetic innervation in rabbits using 11C-hydroxyephedrine PET: relation to 123I-MIBG uptake
Authors: Yusuke Nomura
Ichiro Matsunari
Hiroyuki Takamatsu
Yoshihiro Murakami
Takahiro Matsuya
Junichi Taki
Kenichi Nakajima
Stephan G. Nekolla
Wei-Ping Chen
Kouji Kajinami
Publication date: 01-08-2006
Publisher: Springer-Verlag
Published in: European Journal of Nuclear Medicine and Molecular Imaging / Issue 8/2006
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-006-0105-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Quantitation of cardiac sympathetic innervation in rabbits using ¹¹C-hydroxyephedrine PET: relation to ¹²³I-MIBG uptake

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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