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01-10-2015 | Original Communication

Quantification of CSF biomarkers using an electrochemiluminescence-based detection system in the differential diagnosis of AD and sCJD

Authors: Franc Llorens, Niels Kruse, Matthias Schmitz, Mohsin Shafiq, José Eriton Gomes da Cunha, Nadine Gotzman, Saima Zafar, Katrin Thune, João Ricardo Mendes de Oliveira, Brit Mollenhauer, Inga Zerr

Published in: Journal of Neurology | Issue 10/2015

Abstract

The identification of reliable diagnostic tools for the differential diagnosis between sporadic Creutzfeldt–Jakob Disease (sCJD) and Alzheimer’s disease (AD) remains impeded by the existing clinical, neuropathological and molecular overlap between both diseases. The development of new tools for the quantitative measurement of biomarkers is gaining experimental momentum due to recent advances in high-throughput screening analysis and with the optimization of assays for their quantification in biological fluids, including cerebrospinal fluid (CSF). Electrochemiluminescence (ECL)-based immunoassays have demonstrated to achieve clinical quality performance in a variety of sample types due to its high sensitivity and dynamic range. Here, we quantified the CSF levels of Tau-protein, β-amyloid 1-42 (Aβ42) and α-synuclein, as important biomarkers in CSF used in the differential diagnosis of neurodegenerative disorders in 12 AD, 12 sCJD and 12 control cases by singleplex ECL-based technology. Its performance has been compared to classical enzyme-linked immunosorbent assays (ELISA) to confront their clinical accuracy. ECL-based technology validates previous data obtained with ELISA and presents a higher performance in the discrimination of three analysed groups as determined by increased area under the curve (AUC) values for the three biomarkers. Importantly, α-synuclein levels detected by ECL allow an excellent discrimination between sCJD cases and AD and control cases, unveiling a new clinical approach for the differential diagnosis of sCJD.

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Title: Quantification of CSF biomarkers using an electrochemiluminescence-based detection system in the differential diagnosis of AD and sCJD
Authors: Franc Llorens
Niels Kruse
Matthias Schmitz
Mohsin Shafiq
José Eriton Gomes da Cunha
Nadine Gotzman
Saima Zafar
Katrin Thune
João Ricardo Mendes de Oliveira
Brit Mollenhauer
Inga Zerr
Publication date: 01-10-2015
Publisher: Springer Berlin Heidelberg
Published in: Journal of Neurology / Issue 10/2015
Print ISSN: 0340-5354
Electronic ISSN: 1432-1459
DOI: https://doi.org/10.1007/s00415-015-7837-x

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Springer Medicine

Quantification of CSF biomarkers using an electrochemiluminescence-based detection system in the differential diagnosis of AD and sCJD

Abstract

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Abstract

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