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Critical Care
Published in: Critical Care 1/2009

Open Access 01-02-2009 | Research

Pulmonary vascular permeability changes in an ovine model of methicillin-resistant Staphylococcus aureus sepsis

Authors: Collette C Jonkam, Kamna Bansal, Daniel L Traber, Atsumori Hamahata, Marc O Maybauer, Dirk M Maybauer, Robert A Cox, Matthias Lange, Rhykka L Connelly, Lillian D Traber, Clarisse D Djukom, John R Salsbury, David N Herndon, Perenlei Enkhbaatar

Published in: Critical Care | Issue 1/2009

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Abstract

Introduction

Endothelial dysfunction is a hallmark of sepsis, associated with lung transvascular fluid flux and pulmonary dysfunction in septic patients. We tested the hypothesis that methicillin-resistant Staphylococcus aureus (MRSA) sepsis following smoke inhalation increases pulmonary transvascular fluid flux via excessive nitric oxide (NO) production.

Methods

Ewes were chronically instrumented, and randomised into either a control or MRSA sepsis (MRSA and smoke inhalation) group.

Results

Pulmonary function remained stable in the control group, whereas the MRSA sepsis group developed impaired gas exchange and significantly increased lung lymph flow, permeability index and bloodless wet-to-dry weight-ratio (W/D ratio). The plasma nitrate/nitrite (NOx) levels, lung inducible nitric oxide synthases (iNOS) and endothelial nitric oxide synthases (eNOS), vascular endothelial growth factor (VEGF) protein expressions and poly-(ADP)-ribose (PAR) were significantly increased by MRSA challenge.

Conclusions

These results provide evidence that excessive NO production may mediate pulmonary vascular hyperpermeability in MRSA sepsis via up regulation of reactive radicals and VEGF.
Appendix
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Metadata
Title
Pulmonary vascular permeability changes in an ovine model of methicillin-resistant Staphylococcus aureus sepsis
Authors
Collette C Jonkam
Kamna Bansal
Daniel L Traber
Atsumori Hamahata
Marc O Maybauer
Dirk M Maybauer
Robert A Cox
Matthias Lange
Rhykka L Connelly
Lillian D Traber
Clarisse D Djukom
John R Salsbury
David N Herndon
Perenlei Enkhbaatar
Publication date
01-02-2009
Publisher
BioMed Central
Published in
Critical Care / Issue 1/2009
Electronic ISSN: 1364-8535
DOI
https://doi.org/10.1186/cc7720

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