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BMC Complementary Medicine and Therapies

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Open Access 01-12-2020 | Pseudomonas Aeruginosa | Research article

Antibacterial activity of Centaurea pumilio L. root and aerial part extracts against some multidrug resistant bacteria

Authors: Huda Naeim, Amr El-Hawiet, Raoufa A. Abdel Rahman, Ahmed Hussein, Maha A. El Demellawy, Amira M. Embaby

Published in: BMC Complementary Medicine and Therapies | Issue 1/2020

Abstract

Background

In the context of searching for potent, safe, natural antimicrobial agents to combate the global antimicrobial resistance (AMR) phenomenon, the current study evaluates for the first time ever, the broad-spectrum antimicrobial activity of essential oil (EO) and extracts from the rare wild plant Centaurea pumilio L.. It has tremendous ethnomedicinal values; its dried root is used as a fattening agent, a treatment for bad breath and diabetes, and screened for schistosomicidal activity.

Methods

C. pumilio EO was extracted by hydrodistillation using a Clevenger apparatus. Chemical constituents of aerial part were extracted using a sequential solvent/solvent procedure employing four solvents with increasing polarities in the following order: petroleum ether, chloroform, ethyl acetate, and n-butanol. The chemical constituents were identified by GC-MS. Fifty-two microbial strains were used; twenty-six multidrug resistant (MDR), sixteen clinical, and ten reference strains. The identification of the microbial strains was performed by MALDI-TOF-MS. The antimicrobial activity of the EO and the aerial part and the root extracts was assessed through disc diffusion assay. A minimum inhibitory concentration (MIC) of the EO and extracts was determined using the broth micro-dilution method.

Results

The growth of reference and clinical strains was inhibited by EO, methanol, chloroform, and ethyl acetate aerial part extracts and chloroform root extract. The MDR strains growth, however, was inhibited only by EO and chloroform aerial part extract. GC-MS identified for the first time eighteen constituents from aerial part EO and chloroform extract each. EO showed antimicrobial activity against the reference, clinical, and MDR strains with MIC values of 31.25–125, 31.25–125, and 62.50–250 μg/mL, respectively. Methanol aerial part extract exhibited high antimicrobial activities with MIC values of 62.50–250 μg/mL against reference and clinical strains. Chloroform root extract displayed strong antimicrobial activity against reference and clinical strains recording MIC values of 62.50–250 μg/mL and 62.50–125 μg/mL, respectively. The chloroform aerial part extract demonstrated potent antimicrobial activity against the reference, clinical, and MDR strains with 31.25, 31.25, and 15.62 μg/mL MIC values, respectively.

Conclusions

Present data unravel the C. pumilio pharmacological magnitude to discover eco-friendly potent antimicrobial agents to fight AMR phenomenon.

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Title: Antibacterial activity of Centaurea pumilio L. root and aerial part extracts against some multidrug resistant bacteria
Authors: Huda Naeim
Amr El-Hawiet
Raoufa A. Abdel Rahman
Ahmed Hussein
Maha A. El Demellawy
Amira M. Embaby
Publication date: 01-12-2020
Publisher: BioMed Central
Keyword: Pseudomonas Aeruginosa
Published in: BMC Complementary Medicine and Therapies / Issue 1/2020
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-020-2876-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Antibacterial activity of Centaurea pumilio L. root and aerial part extracts against some multidrug resistant bacteria

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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