Top

Magnetic Resonance Materials in Physics, Biology and Medicine

Published in:

Open Access 01-08-2019 | Research Article

Proton MR spectroscopy of human pancreas allografts

Authors: Jan Weis, Håkan Ahlström, Olle Korsgren

Published in: Magnetic Resonance Materials in Physics, Biology and Medicine | Issue 4/2019

Abstract

Objective

To estimate pancreas graft relaxation times and concentrations of total fat, and the intracellular lipids of non-adipose pancreatic cells (NAPC) using proton (¹H) magnetic resonance spectroscopy (MRS) during cold preservation.

Materials and methods

Grafts from 11 human donors were investigated. Each pancreas was perfused in situ with histidine-tryptophan-ketoglutarate (HTK) or with University of Wisconsin solution and placed into a transport container. Temperature of the grafts was maintained at 4 ± 2 °C during transport to our hospital and MR scanning. A 1.5 T clinical scanner was used for the measurements. Single-voxel PRESS spectra were acquired using transmit–receiver head coil.

Results

Relaxation times were measured for lipid (–CH₂–)_n (T₁, 287 ± 60 ms; T₂, 27 ± 4 ms), and tissue water (T₁, 670 ± 69 ms; T₂, 77 ± 17 ms). Average total fat, and intracellular lipids of NAPC concentrations were 79.2 ± 100.8 (range 2.4–304.4), and 2.9 ± 1.2 mmol/kg ww, respectively.

Conclusion

We have shown that ¹H-MRS is a useful tool for the estimation of pancreas graft lipid concentrations. Total pancreatic fat and especially content of intracellular lipids of NAPC are valuable measures for inspection of graft quality prior to transplantation or islet of Langerhans isolation.

Wahoff DC, Papalois BE, Najarian JS et al (1995) Autologous islet transplantation to prevent diabetes after pancreatic resection. Ann Surg 222:562–579CrossRefPubMedPubMedCentral

Shapiro JAM, Lakey JRT, Ryan EA et al (2000) Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med 343:230–238CrossRefPubMed

Gruessner RWG, Sutherland DER, Gruessner AC (2004) Mortality assessment for pancreas transplants. Am J Transplant 4:2018–2026CrossRefPubMed

White SA, Shaw JA, Sutherland DER (2009) Pancreas transplantation. Lancet 373:1808–1817CrossRefPubMed

Fridell JA, Rogers J, Stratta RJ (2010) The pancreas allograft donor: current status, controversies, and challenges for future. Clin Transplant 24:433–449CrossRefPubMed

Krieger NR, Odorico JS, Heisey DM et al (2003) Underutilization of pancreas donors. Transplantation 75:1271–1276CrossRefPubMed

Carlbom L, Weis J, Johansson L, Korsgren O, Ahlström H (2017) Pre-transplantation 31P-magnetic resonance spectroscopy for quality assessment of human pancreatic grafts—a feasibility study. Magn Reson Imag 39:98–102CrossRef

Scott WE, Weegman BP, Ferrer-Fabrega J et al (2010) Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance. Transplant Proc 42:2011–2015CrossRefPubMedPubMedCentral

Tushuizen ME, Bunck MC, Pouwels PJ et al (2007) Pancreatic fat content and β-cell function in men with and without type 2 diabetes. Diabetes Care 30:2916–2921CrossRefPubMed

10.

Lingvay I, Esser V, Legendre JL et al (2009) Noninvasive quantification of pancreatic fat content in humans. J Clin Endocrinol Metab 94:4070–4076CrossRefPubMedPubMedCentral

11.

Su TH, Jin EH, Shen H, Zhang Y, He W (2012) In vivo proton MRS of normal pancreas metabolites during breath-holding and free-breathing. Clin Radiol 67:633–637CrossRefPubMed

12.

Ouwerkerk R, Gharib AM (2012) ¹H-MRS of pancreatic metabolites. In: Proceedings of the 20th scientific meeting, International Society for Magnetic Resonance in Medicine, Melbourne, p 1373

13.

Gaborit B, Abdesselam I, Kober F et al (2015) Ectopic fat storage in the pancreas using ¹H-MRS: importance of diabetic status and modulation with bariatric surgery-induced weight loss. Int J Obesity 39:480–487CrossRef

14.

Heni M, Machann J, Steiger H et al (2010) Pancreatic fat content is negatively associated with insulin secretion in individuals with impaired fasting glucose and/or impaired glucose tolerance: a nuclear magnetic resonance study. Diabetes Metab Res Rev 26:200–205CrossRefPubMed

15.

Kühn J-P, Berthold F, Mayerle J et al (2015) Pancreatic steatosis demonstrated at MR imaging in the general population: clinical relevance. Radiology 276:129–136CrossRefPubMed

16.

Boesch C (2007) Musculoskeletal spectroscopy. J Magn Reson Imag 25:321–338CrossRef

17.

Szczepaniak LS, Nurenberg P, Leonard D et al (2005) Magnetic resonance spectroscopy to measure hepatic triglyceride content: prevalence of hepatic steatosis in the general population. Am J Physiol Endocrinol Metab 288:E462–E468CrossRefPubMed

18.

Szczepaniak LS, Dobbins RL, Metzger GJ et al (2003) Myocardial triglycerides and systolic function in humans: in vivo evaluation by localized proton spectroscopy and cardiac imaging. Magn Reson Med 49:417–423CrossRefPubMed

19.

Vanhamme L, van den Boogaart A, Van Huffel S (1997) Improved method for accurate and efficient quantification of MRS data with use of prior knowledge. J Magn Reson 129:35–43CrossRefPubMed

20.

Naressi A, Couturier C, Devos JM et al (2001) Java-based graphical user interface for the MRUI quantitation package. MAGMA 12:141–152CrossRefPubMed

21.

Weis J, Johansson L, Ortiz-Nieto F, Ahlström H (2009) Assessment of lipids in skeletal muscle by LCModel and AMARES. J Magn Reson Imag 30:1124–1129CrossRef

22.

Barker PB, Soher BJ, Blackband SJ, Chatham JC, Mathews VP, Bryan RN (1993) Quantification of proton NMR spectra of the human brain using tissue water as an internal concentration reference. NMR Biomed 6:89–94CrossRefPubMed

23.

Boesch C, Machann J, Vermathen P, Schick F (2006) Role of proton MR for the study of muscle lipid metabolism. NMR Biomed 19:968–988CrossRefPubMed

24.

Eckhard M, Brendel MD, Brandhorst D, Brandhorst H, Bretzel RG (2004) Can the density of native pancreatic tissue slices predict human islet isolation and purification outcome? Transplant Proc 36:2845–2848CrossRefPubMed

25.

Heerschap A, Jager GJ, Van der Graaf M, Barentsz JO, Ruijs SH (1997) Proton MR spectroscopy of the normal human prostate with an endorectal coil and a double spin-echo pulse sequence. Magn Reson Med 37:204–213CrossRefPubMed

26.

Misra D, Gupta V, Sonkar AA, Bajpai U, Roy R (2008) Proton HR-MAS NMR spectroscopic characterization of metabolites in various human organ tissues: pancreas, brain and liver from trauma cases. Physiol Chem Phys Med NMR 40:67–88PubMed

27.

Weis J, Kullberg J, Ahlström H (2018) Multiple breath-hold proton spectroscopy of human liver at 3 T: relaxation times and concentration of glycogen, choline, and lipids. J Magn Reson Imag 47:410–417CrossRef

28.

De Bazelaire CMJ, Duhamel GD, Rofsky NM, Alsop DC (2004) MR imaging relaxation time of abdominal and pelvic tissues measured in vivo at 3.0 T: preliminary results. Radiology 230:652–659CrossRefPubMed

29.

Rakow-Penner R, Daniel B, Yu H, Sawyer-Glover A, Glover GH (2006) Relaxation times of breast tissue at 1.5 T and 3 T measured using IDEAL. J Magn Reson Imag 23:87–91CrossRef

30.

Baron P, Deckers R, Knuttel FM, Bartels LW (2015) T₁ and T₂ temperature dependence of female human breast adipose tissue at 1.5 T: groundwork for monitoring thermal therapies in the breast. NMR Biomed 28:1463–1470CrossRefPubMed

31.

Livingstone RS, Begovatz P, Kahl S et al (2014) Initial clinical application of modified Dixon with flexible echo times: hepatic and pancreatic fat assessment in comparison with ¹H-MRS. Magn Reson Mater Phy 27:397–405CrossRef

32.

Zhou YP, Grill VE (1994) Long-term exposure of rat pancreatic islets to fatty acids inhibits glucose-induced insulin secretion and biosynthesis through a glucose fatty acid cycle. J Clin Invest 93:870–876CrossRefPubMedPubMedCentral

33.

Schaffer JE (2003) Lipotoxicity: when tissues overeat. Curr Opin Lipidol 14:281–287CrossRefPubMed

34.

McGarry JD, Dobbins R (1999) Fatty acids, lipotoxicity and insulin secretion. Diabetologia 42:128–138CrossRefPubMed

35.

Hwang JH, Pan JW, Heydari S, Hetherington HP, Stein DT (2001) Regional differences in intramyocellular lipids in humans observed by in vivo ¹H-MR spectroscopic imaging. J Appl Physiol 90:1267–1274CrossRefPubMed

36.

Howald H, Boesch C, Kreis R et al (2002) Content of intramyocellular lipids derived by electron microscopy, biochemical assays, and ¹H-MR spectroscopy. J Appl Physiol 92:2264–2272CrossRefPubMed

Title: Proton MR spectroscopy of human pancreas allografts
Authors: Jan Weis
Håkan Ahlström
Olle Korsgren
Publication date: 01-08-2019
Publisher: Springer International Publishing
Published in: Magnetic Resonance Materials in Physics, Biology and Medicine / Issue 4/2019
Print ISSN: 0968-5243
Electronic ISSN: 1352-8661
DOI: https://doi.org/10.1007/s10334-019-00740-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Proton MR spectroscopy of human pancreas allografts

Abstract

Objective

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 4/2019

Finite difference transmission line model for the design of safe multi-section cables in MRI

Chemotherapy response of pancreatic cancer by diffusion-weighted imaging (DWI) and intravoxel incoherent motion DWI (IVIM-DWI) in an orthotopic mouse model

Detection of axonal degeneration in a mouse model of Huntington’s disease: comparison between diffusion tensor imaging and anomalous diffusion metrics

White and grey matter development in utero assessed using motion-corrected diffusion tensor imaging and its comparison to ex utero measures

Fast and accurate compensation of signal offset for T2 mapping

Gadolinium (III) oxide nanoparticles coated with folic acid-functionalized poly(β-cyclodextrin-co-pentetic acid) as a biocompatible targeted nano-contrast agent for cancer diagnostic: in vitro and in vivo studies