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Open Access 01-01-2014 | Original Article

Proteomic analysis of the abdominal aortic aneurysm wall

Authors: Jiri Molacek, Jan Mares, Vladislav Treska, Karel Houdek, Jan Baxa

Published in: Surgery Today | Issue 1/2014

Abstract

Purposes

A ruptured AAA (rAAA) is a common cause of death in males over 60 years of age, and the global mortality from rAAA exceeds 80 %. The pathological processes occurring in the wall of the developing AAA are still unclear. The potential pathophysiological mechanisms underlying aortic aneurysms have been examined by many studies using immunohistochemistry and were, therefore, targeted at specific, preselected protein antigens.

Methods

We collected samples of tissue from anterior wall of an aneurysm sac from 15 patients indicated for AAA resection (group A) during the period from 2010 to 2011. These samples were subjected to a proteomic analysis. In addition, we collected control samples of identical aortic tissue from 10 heart-beating deceased organ donors (group B).

Results

A total of 417 differentially expressed protein fractions were identified, 18 of which were only detected in the healthy controls, while 85 were specific for aneurysm tissue and 314 were detectable in both groups. In 175 protein fractions, the gel-derived spot volumes differed significantly between aneurismal and healthy aortic tissue.

Conclusions

We found a significant difference in the proteome of the AAA tissue and non-dilated aortic tissue. We demonstrated that the AAA proteome is considerably richer and more varied than the healthy and atherosclerotic aorta. We believe that our results clearly demonstrate a completely different etiopathogenesis of atherosclerosis and aneurismal disease.

Michel JB, Martin-Ventura JL, Egido J, Sakalihasan N, Treska V, Lindholt J, Allaire E, Thorsteinsdottir U, Cockerill G, Swedenborg J. Novel aspects of the pathogenesis of aneurysms of the abdominal aorta in humans. FAD EU consortium. Cardiovasc Res. 2011;90(1):18–27. [Epub 2010 Oct 30].PubMedCrossRef

Urbonavicius S, Lindholt JS, Delbosc S, Urbonaviciene G, Henneberg EW, Vorum H, Meilhac O, Honoré B. Proteins associated with the size and expansion rate of the abdominal aortic aneurysm wall as identified by proteomic analysis. Interact Cardiovasc Thorac Surg. 2010;11(4):433–41. [Epub 2010 Jul 30].PubMedCrossRef

Spadaccio C, Di Domenico F, Perluigi M, Lusini M, Giorgi A, Schininà ME, Blarzino C, Covino E, Chello M, Coccia R. Serum proteomics in patients with diagnosis of abdominal aortic aneurysm. Cardiovasc Pathol. [Epub ahead of print].

Blanco-Colio LM, López JA, Martínez-Pinna Albar R, Egido J, Martín-Ventura JL. Vascular proteomics, a translational approach: from traditional to novel proteomic techniques. Expert Rev Proteomics. 2009;6(5):461–4.PubMedCrossRef

Sachon E, Matheron L, Clodic G, Blascoa T, Bolbacha G. MALDI TOF–TOF characterization of a light stabilizer polymer contaminant from polypropylene or polyethylene plastic test tubes. J Mass Spectrom. 2010;45(1):43–50.

Pulinx B, Hellenthal FA, Hamulyák K, van Dieijen-Visser MP, Schurink GW, Wodzig WK. Differential protein expression in serum of abdominal aortic aneurysm patients—a proteomic approach. Eur J Vasc Endovasc Surg. 2011;42(5):563–70.PubMedCrossRef

Jelski W, Orywal K, Panek B, Gacko M, Mroczko B, Szmitkowski M. The activity of class I, II, III and IV of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in the wall of abdominal aortic aneurysms. Exp Mol Pathol. 2009;87(1):59–62.PubMedCrossRef

Hayashi T, Morishita E, Ohtake H, Oda Y, Ohta K, Arahata M, Kadohira Y, Maekawa M, Ontachi Y, Yamazaki M, Asakura H, Takami A, Nakao S. Expression of annexin II in human atherosclerotic abdominal aortic aneurysms. Thromb Res. 2008;123(2):274–80.PubMedCrossRef

Tamarina NA, Grassi MA, Johnson DA, Pearce WH. Proteoglycan gene expression is decreased in abdominal aortic aneurysms. J Surg Res. 1998;74(1):76–80.PubMedCrossRef

10.

Kosanam H, Makawita S, Judd B, Newman A, Diamandis EP. Mining the malignant ascites proteome for pancreatic cancer biomarkers. Proteomics. 2011;11(23):4551–8. [Epub 2011 Oct 28].PubMedCrossRef

11.

Atahan K, Küpeli H, Gür S, Yiğitbaşı T, Baskın Y, Yiğit S, Deniz M, Cökmez A, Tarcan E. The value of serum biomarkers (Bc1, Bc2, Bc3) in the diagnosis of early breast cancer. Int J Med Sci. 2011;8(2):148–55.PubMedCentralPubMedCrossRef

12.

Schaaij-Visser TB, Brakenhoff RH, Leemans CR, Heck AJ, Slijper M. Protein biomarker discovery for head and neck cancer. J Proteomics. 2010;73(10):1790–803. [Epub 2010 Feb 4].PubMedCrossRef

13.

Urbonavicius S, Lindholt JS, Vorum H, Urbonaviciene G, Henneberg EW, Honoré B. Proteomic identification of differentially expressed proteins in aortic wall of patients with ruptured and nonruptured abdominal aortic aneurysms. J Vasc Surg. 2009;49(2):455–63. [Epub 2008 Nov 28].PubMedCrossRef

14.

Ando T, Nagai K, Chikada M, Okamoto K, Kurokawa M, Kobayashi T, Kato T, Makuuchi HJ. Proteomic analyses of aortic wall in patients with abdominal aortic aneurysm. Cardiovasc Surg (Torino). 2011;52(4):545–55.

15.

Molacek J, Baxa J, Houdek K, Treska V, Ferda J. Assessment of abdominal aortic aneurysm wall distensibility with electrocardiography-gated computed tomography. Ann Vasc Surg. 2011;25(8):1036–42.PubMedCrossRef

16.

Nordon IM, Brar R, Hinchliffe RJ, Cockerill G, Thompson MM. Proteomics and pitfalls in the search for potential biomarkers of abdominal aortic aneurysms. Vascular. 2010;18(5):264–8.PubMedCrossRef

17.

Martinez-Pinna R, Ramos-Mozo P, Madrigal-Matute J, Blanco-Colio LM, Lopez JA, Calvo E, Camafeita E, Lindholt JS, Meilhac O, Delbosc S, Michel JB, de Ceniga MV, Egido J, Martin-Ventura JL. Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm. Arterioscler Thromb Vasc Biol. 2011;31(4):935–43. [Epub 2011 Jan 27].PubMedCrossRef

Title: Proteomic analysis of the abdominal aortic aneurysm wall
Authors: Jiri Molacek
Jan Mares
Vladislav Treska
Karel Houdek
Jan Baxa
Publication date: 01-01-2014
Publisher: Springer Japan
Published in: Surgery Today / Issue 1/2014
Print ISSN: 0941-1291
Electronic ISSN: 1436-2813
DOI: https://doi.org/10.1007/s00595-012-0480-6

At a glance: The STEP trials

Springer Medicine

Proteomic analysis of the abdominal aortic aneurysm wall

Abstract

Purposes

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purposes

Methods

Results

Conclusions

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Acknowledgment to reviewers

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