Top

Published in:

01-04-2009

Protective Roles of Hydroxyethyl Starch 130/0.4 in Intestinal Inflammatory Response and Oxidative Stress After Hemorrhagic Shock and Resuscitation in Rats

Authors: Pengfei Wang, Yousheng Li, Jieshou Li

Published in: Inflammation | Issue 2/2009

Abstract

This study was designed to determine the effects of various resuscitation fluids on intestinal injuries after hemorrhagic shock and resuscitation (HS/R) and to determine the potential mechanisms. We induced HS by bleeding male Sprague-Dawley rats to a blood pressure of 30 to 40 mmHg for 60 min. Sixty minutes later, the rats were killed (HS group) or immediately resuscitated with L-isomer lactated Ringer’s solution (HS + LR group), shed blood (HS + BL group), or hydroxyethyl starch (HS + HES group) to maintain the blood pressure to the original value during the 60-min resuscitation period. Three hour after resuscitation, bacterial translocation (BT), intestinal permeability, ileal levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, malondialdehyde (MDA), oxidized and reduced glutathione (GSH and GSSG), myeloperoxidase (MPO) activity, nuclear factor (NF)-κB, activator protein (AP)-1 activation, and ileal microscopic and ultrastructural histological changes were measured. Another experiment was designed for survival study of 24 h. HES 130/0.4 solution was as effective as shed blood, required a small volume requirement to restore circulation, and significantly reduced HS/R-induced ileal villous morphological injuries with an anti-inflammatory effect, as reflected by a reduction of TNF-α, IL-6, MPO activity, and NF-κB activation. In addition, HES resuscitation also reduced intestinal permeability and BT and caused less oxidative stress as reflected by a reduction of MDA, GSSG/GSH and AP-1 activation along with restored GSH, whereas shed blood couldn’t. No significant difference was observed in outcome among groups. HES 130/0.4 resuscitation prevents HS/R induced intestinal injury by modulating inflammatory response and preventing oxidative stress in a rat model of hemorrhagic shock. These physiological protective effects appear to be mediated by down-regulation of the transcription factor NF-κB and AP-1.

Lee, C. C., K. A. Marill, W. A. Carter, and R. S. Crupi. 2001. A current concept of trauma-induced multiorgan failure. Ann. Emerg. Med. 38:170–476 doi:10.1067/mem.2001.114313.PubMedCrossRef

Lee, C. C., I. J. Chang, Z. S. Yen, C. Y. Hsu, S. Y. Chen, C. P. Su, W. C. Chiang, S. C. Chen, and W. J. Chen. 2007. Delayed fluid resuscitation in hemorrhagic shock induces proinflammatory cytokine response. Ann. Emerg. Med. 49(1):37–44 doi:10.1016/j.annemergmed.2006.05.031.PubMedCrossRef

Tsai, M. C., W. J. Chen, C. H. Ching, and J. I. Chuang. 2007. Resuscitation with hydroxyethyl starch solution prevents nuclear factor kappaB activation and oxidative stress after hemorrhagic shock and resuscitation in rats. Shock. 27(5):527–33 doi:10.1097/01.shk.0000245032.31859.f2.PubMedCrossRef

Imm, A., and R. W. Carlson. 1993. Fluid resuscitation in circulatory shock. Crit. Care. Clin. 9:313–333.PubMed

Deitch, E. A., J. Morrison, R. Berg, and R. D. Specian. 1990. Effect of hemorrhagic shock on bacterial translocation, intestinal morphology, and intestinal permeability in conventional and antibiotic-decontaminated rats. Crit. Care Med. 18:529 doi:10.1097/00003246-199005000-00014.PubMedCrossRef

Tian, J., X. Lin, R. Guan, and J. G. Xu. 2004. The effects of hydroxyethyl starch on lung capillary permeability in endotoxic rats and possible mechanisms. Anesth. Analg. 98(3):768–774 doi:10.1213/01.ANE.0000099720.25581.86.PubMedCrossRef

Lv, R., W. Zhou, L. D. Zhang, and J. G. Xu. 2005. Effects of hydroxyethyl starch on hepatic production of cytokines and activation of transcription factors in lipopolysaccharide-administered rats. Acta Anaesthesiol Scand. 49(5):635–642 doi:10.1111/j.1399-6576.2005.00668.x.PubMedCrossRef

Feng, X., W. Yan, Z. Wang, J. Liu, M. Yu, S. Zhu, and J. Xu. 2007. Hydroxyethyl starch, but not modified fluid gelatin, affects inflammatory response in a rat model of polymicrobial sepsis with capillary leakage. Anesth. Analg. 104:624–630 doi:10.1213/01.ane.0000250366.48705.96.PubMedCrossRef

Jungheinrich, C., W. Sauermann, F. Bepperling, and N. H. Vogt. 2004. Volume efficacy and reduced influence on measures of coagulation using hydroxyethyl starch 130/0.4 (6%) with an optimised in vivo molecular weight in orthopaedic surgery: a randomised, double-blind study. Drugs R D. 5:1–9 doi:10.2165/00126839-200405010-00001.PubMedCrossRef

10.

Wattanasirichaigoon, S., M. J. Menconi, R. L. Delude, and M. P. Fink. 1999. Effect of mesenteric ischemia and reperfusion or hemorrhagic shock on intestinal mucosal permeability and ATP content in rats. Shock. 12:127–133 doi:10.1097/00024382-199908000-00006.PubMedCrossRef

11.

Mihara, M., and M. Uchiyama. 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 86:271–278 doi:10.1016/0003-2697(78)90342-1.PubMedCrossRef

12.

Chen, S. T., J. I. Chuang, M. H. Hong, and E. I. C. Li. 2002. Melatonin attenuates MPP+-induced neurodegeneration and glutathione impairment in nigrostriatal dopaminergic pathway. J. Pineal Res. 32:262–269 doi:10.1034/j.1600-079X.2002.01871.x.PubMedCrossRef

13.

Suzuki, K., H. Ota, S. Sasagawa, T. Sakatani, and T. Fujikura. 1983. Assay method for myeloperoxidase in human polymorphonuclear leukocytes. Anal Biochem. 132:345–352 doi:10.1016/0003-2697(83)90019-2.PubMedCrossRef

14.

Chiu, C. J., A. H. McArdle, R. Brown, H. J. Scott, and F. N. Gurd. 1970. Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch. Surg. 101(4):478–483.PubMed

15.

Kaszaki, J., A. Wolfárd, L. Szalay, and M. Boros. 2006. Pathophysiology of ischemia-reperfusion injury. Transplant Proc. 38(3):826–828 doi:10.1016/j.transproceed.2006.02.152.PubMedCrossRef

16.

Nemeth, I., and D. Boda. 1989. The ratio of oxidized/reduced glutathione as an index of oxidative stress in various experimental models of shock syndrome. Biomed. Biochim. Acta. 48:53–57.

17.

Schnoor, J., R. Schreck, J. H. Baumert, C. Grosse-Siestrup, R. Rossaint, and J. K. Unger. 2004. Influence of differences in body weight and volume management on experimental results in porcine models. Int. J. Artif. Organs. 27:924–934.PubMed

18.

Shi, H. P., E. A. Deitch, D. Z. Xu, Q. Lu, and C. J. Hauser. 2002. Hypertonic saline improves intestinal mucosal barrier function and lung injury after trauma-hemorrhagic shock. Shock. 17:496–501 doi:10.1097/00024382-200206000-00010.PubMedCrossRef

19.

Rhee, P., D. Burris, C. Kaufmann, et al. 1998. Lactated Ringer’s solution resuscitation causes neutrophil activation after hemorrhagic shock. J Trauma. 44:313–319 doi:10.1097/00005373-199802000-00014.PubMedCrossRef

20.

Ferreira, E. L., R. G. Terzi, W. A. Silva, and A. C. de Moraes. 2005. Early colloid replacement therapy in a near-fatal model of hemorrhagic shock. Anesth. Analg. 101(6):1785–1791 doi:10.1213/01.ANE.0000184133.48569.55.PubMedCrossRef

21.

Marx, G., M. Cobas Meyer, T. Schuerholz, B. Vangerow, K. F. Gratz, H. Hecker, R. Sumpelmann, H. Rueckoldt, and M. Leuwer. 2002. Hydroxyethyl starch and modified fluid gelatin maintain plasma volume in a porcine model of septic shock with capillary leakage. Intensive Care Med. 28:629–35 doi:10.1007/s00134-002-1260-3.PubMedCrossRef

22.

Taniguchi, T., Y. Koido, J. Aiboshi, T. Yamashita, S. Suzaki, and A. Kurokawa. 1999. The ratio of interleukin-6 to interleukin-10 correlates with severity in patients with chest and abdominal trauma. Am. J. Emerg. Med. 17:548–551 doi:10.1016/S0735-6757(99)90194-8.PubMedCrossRef

23.

Feng, X., J. Liu, M. Yu, S. Zhu, and J. Xu. 2007. Protective roles of hydroxyethyl starch 130/0.4 in intestinal inflammatory response and survival in rats challenged with polymicrobial sepsis. Clin. Chim. Acta. 376(1–2):60–67 doi:10.1016/j.cca.2006.07.008.PubMedCrossRef

24.

Fink, M. P. 2002. Reactive oxygen species as mediators of organ dysfunction caused by sepsis, acute respiratory distress syndrome, or hemorrhagic shock: potential benefits of resuscitation with Ringer’s ethyl pyruvate solution. Curr. Opin. Clin. Nutr. Metab. Care. 5:167–174 doi:10.1097/00075197-200203000-00009.PubMedCrossRef

25.

Granger, D. N., J. N. Benoit, M. Suzuki, and M. B. Grisham. 1989. Leukocyte adherence to venular endothelium during ischemia-reperfusion. Am. J. Physiol. 257:683–688.

26.

Childs, E. W., K. F. Udobi, J. G. Wood, F. A. Hunter, D. M. Smalley, and L. Y. Cheung. 2002. In vivo visualization of reactive oxidants and leukocyte-endothelial adherence following hemorrhagic shock. Shock. 18:423–427 doi:10.1097/00024382-200211000-00006.PubMedCrossRef

27.

Sener, G., A. O. Sehirli, H. Satiroğlu, M. Keyer-Uysal, and B. C Yeğen. 2002. Melatonin improves oxidative organ damage in a rat model of thermal injury. Burns. 28(5):419–425 doi:10.1016/S0305-4179(02)00053-0.PubMedCrossRef

28.

Therade-Matharan, S., E. Laemmel, J. Duranteau, and E. Vicaut. 2004. Reoxygenation after hypoxia and glucose depletion causes reactive oxygen species production by mitochondria in HUVEC. Am. J. Physiol. Regul. Integr. Comp. Physiol. 287:1037–1043 doi:10.1152/ajpregu.00048.2004.

29.

Karabeyoğlu, M., B. Unal, B. Bozkurt, I. Dolapçi, A. Bilgihan, I. Karabeyoğlu, and O. Cengiz. 2008. The effect of ethyl pyruvate on oxidative stress in intestine and bacterial translocation after thermal injury. J. Surg. Res. 144(1):59–63 doi:10.1016/j.jss.2007.02.050.PubMedCrossRef

30.

Mori, T., H. Yamamoto, T. Tabata, T. Shimizu, Y. Endo, K. Hanasawa, M. Fujimiya, and T. Tani. 2005. A free radical scavenger, edaravone (MCI-186), diminishes intestinal neutrophil lipid peroxidation and bacterial translocation in a rat hemorrhagic shock model. Crit. Care. Med. 33(5):1064–1069 doi:10.1097/01.CCM.0000162952.14590.EC.PubMedCrossRef

31.

Vega, D., C. D. Badami, F. J. Caputo, A. C. Watkins, Q. Lu, Z. Xu da, T. L. Berezina, S. B. Zaets, E. Feketeova, and E. A. Deitch. 2008. The influence of the type of resuscitation fluid on gut injury and distant organ injury in a rat model of trauma/hemorrhagic shock. J. Trauma. 65(2):409–414 discussion 414–5.PubMedCrossRef

32.

Childs, E. W., J. G. Wood, D. M. Smalley, F. A. Hunter, and L. Y. Cheung. 1999. Leukocyte adherence and sequestration following hemorrhagic shock and total ischemia in rats. Shock. 11(4):248–252 doi:10.1097/00024382-199904000-00004.PubMedCrossRef

33.

Paxian, M., I. Bauer, D. Kaplan, M. Bauer, and H. Rensing. 2002. Hepatic redox regulation of transcription factors activator protein-1 and nuclear factor-kB after hemorrhagic shock in vivo. Antioxid Redox Signal. 4(5):711–20 doi:10.1089/152308602760598855.PubMedCrossRef

34.

Sha, W. C. 1998. Regulation of immune responses by NF-κB/Rel transcription factor. J. Exp. Med. 187:143–146 doi:10.1084/jem.187.2.143.PubMedCrossRef

35.

Altavilla, D., A. Saitta, G. Squadrito, M. Galeano, S. F. Venuti, S. Guarini, C. Bazzani, A. Bertolini, A. P. Caputi, and F. Squadrito. 2002. Evidence for a role of nuclear factor-kB in acute hypovolemic hemorrhagic shock. Surgery. 131:50–58 doi:10.1067/msy.2002.118320.PubMedCrossRef

36.

Sham, B. D., H. H. Barton, L. L. Reznikov, C. B. Cairns, A. Banerjee, A. H. Harken, and X. Meng. 2002. Ischemia alone is sufficient to induce TNF-alpha mRNA and peptide in the myocardium. Shock. 17:114–119 doi:10.1097/00024382-200202000-00006.CrossRef

37.

Rensing, H., H. Jaeschke, I. Bauer, C. Pätau, V. Datene, B. H. Pannen, and M. Bauer. 2001. Differential activation pattern of redox-sensitive transcription factors and stress-inducible dilator systems heme oxygenase-1 and inducible nitric oxide synthase in hemorrhagic and endotoxic shock. Crit Care Med. 29(10):1962–1971 doi:10.1097/00003246-200110000-00019.PubMedCrossRef

38.

Domenicotti, C., D. Paola, A. Vitali, M. Nitti, C. D’Abramo, D. Cottalasso, G. Maloberti, F. Biasi, G. Poli, E. Chiarpotto, U. M. Marinari, and M. A. Pronzato. 2000. Glutathione depletion induces apoptosis of rat hepatocytes through activationof protein kinase C novel isoforms and dependent increase in AP-1 nuclear binding. Free Radic. Biol. Med. 29:1280–1290 doi:10.1016/S0891-5849(00)00429-9.PubMedCrossRef

Title: Protective Roles of Hydroxyethyl Starch 130/0.4 in Intestinal Inflammatory Response and Oxidative Stress After Hemorrhagic Shock and Resuscitation in Rats
Authors: Pengfei Wang
Yousheng Li
Jieshou Li
Publication date: 01-04-2009
Publisher: Springer US
Published in: Inflammation / Issue 2/2009
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-009-9105-7

Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2009

Effects of Trefoil Peptide 3 on Expression of TNF-α, TLR4, and NF-κB in Trinitrobenzene Sulphonic Acid Induced Colitis Mice

CC16 Inhibits the Migration of Eosinophils Towards the Formyl Peptide fMLF but not Towards PGD2

Repeated Systemic Escherichia coli Infection Enhances Anti-oxidant Response in Hypercholesterolemic Mice Inducing Cardiovascular Inflammation

Inhalation of Carbon Monoxide Ameliorates Collagen-induced Arthritis in Mice and Regulates the Articular Expression of IL-1β and MCP-1

Efficacy and Mechanism of Salvia Miltiorrhizae Injection in the Treatment of Rats with Severe Acute Pancreatitis

Beyond Interstitial Lung Disease: The Rapidly Evolving Role of Calgranulin B as a Biomarker of Systemic Malignancies

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

At a glance: The STEP trials