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Journal of Orthopaedic Surgery and Research

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Open Access 01-12-2017 | Research article

Protective effect of hydrogen-saturated saline on acute lung injury induced by oleic acid in rats

Authors: Youguo Ying, Haizhou Xu, Min Yao, Zonghe Qin

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2017

Abstract

Background

The purpose of the study is to investigate the role and mechanisms of hydrogen-saturated saline (HSS) in the acute lung injury (ALI) induced by oleic acid (OA) in rats.

Methods

Rats were treated with OA (0.1 mL/kg) to induce ALI and then administered with HSS (5 mL/kg) by intravenous (iv) and intraperitoneal (ip) injection, respectively. Three hours after the injection with OA, the arterial oxygen partial pressure (PaO₂), arterial oxygen saturation (SaO₂), carbon dioxide partial pressure (PaCO₂), and bicarbonate (HCO₃ ⁻) levels were analyzed using blood gas analyzer. In addition, the levels of malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), and interleukin 1β (IL-1β) and myeloperoxidase (MPO) activity were measured by commercial kits, and pathological changes of lung tissue were examined by HE staining. Finally, the correlations of MPO activity or MDA level with the levels of TNF-α or IL-1β were analyzed by Pearson’s correlation analysis.

Results

We found decreased PaO₂ levels and the pathological changes of lung tissue of ALI after OA injection. In addition, OA increased the levels of MDA, TNF-α, and IL-1β, as well as MPO activity in lung tissues (P < 0.05). However, after treatment with HSS, all of these changes were alleviated (P < 0.05), and these changes were mitigated when treated with HSS by ip then iv injection (P < 0.05). Furthermore, MDA level and MPO activity were positively correlated with TNF-α and IL-1β levels in the lung tissue, respectively (P < 0.01).

Conclusion

HSS attenuated ALI induced by OA in rats and might protect against ALI through selective resistance to oxidation and inhibiting inflammatory infiltration.

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Title: Protective effect of hydrogen-saturated saline on acute lung injury induced by oleic acid in rats
Authors: Youguo Ying
Haizhou Xu
Min Yao
Zonghe Qin
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Journal of Orthopaedic Surgery and Research / Issue 1/2017
Electronic ISSN: 1749-799X
DOI: https://doi.org/10.1186/s13018-017-0633-9

At a glance: The STEP trials

Springer Medicine

Protective effect of hydrogen-saturated saline on acute lung injury induced by oleic acid in rats

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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