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BMC Complementary Medicine and Therapies

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Open Access 01-12-2013 | Research article

Protective activity of Panduratin A against Thioacetamide-induced oxidative damage: demonstration with in vitro experiments using WRL-68 liver cell line

Authors: Suzy M Salama, Ahmed S AlRashdi, Mahmood A Abdulla, Pouya Hassandarvish, Mehmet Bilgen

Published in: BMC Complementary Medicine and Therapies | Issue 1/2013

Abstract

Background

Chalcone Panduratin A (PA) has been known for its antioxidant property, but its merits against oxidative damage in liver cells has yet to be investigated. Hence, the paper aimed at accomplishing this task with normal embryonic cell line WRL-68.

Methods

PA was isolated from Boesenbergia rotunda rhizomes and its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric reducing power (FRAP) activities were measured in comparison with that of the standard reference drug Silymarin (SI). Oxidative damage was induced by treating the cells with 0.04 g/ml of toxic thioacetamide for 60 minutes followed by treatment with 1, 10 and 100 μg/ml concentrations of either PA or SI. The severities of oxidative stress in the control and experimental groups of cells were measured by Malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities.

Results

PA exhibited an acceptable DPPH scavenging and FRAP activities close to that of Silymarin. Treating the injured cells with PA significantly reduced the MDA level and increased the cell viability, comparable to SI. The activities of SOD, CAT and GPx were significantly elevated in the PA-treated cells in a dose dependent manner and again similar to SI.

Conclusion

Collectively, data suggested that PA has capacity to protect normal liver cells from oxidative damage, most likely via its antioxidant scavenging ability.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/13/279/prepub

Title: Protective activity of Panduratin A against Thioacetamide-induced oxidative damage: demonstration with in vitro experiments using WRL-68 liver cell line
Authors: Suzy M Salama
Ahmed S AlRashdi
Mahmood A Abdulla
Pouya Hassandarvish
Mehmet Bilgen
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2013
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-13-279

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Protective activity of Panduratin A against Thioacetamide-induced oxidative damage: demonstration with in vitro experiments using WRL-68 liver cell line

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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