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Breast Cancer Research
Published in: Breast Cancer Research 1/2000

01-03-2000 | Meeting abstract

Promising method for DNA extraction from paraffin embedded archive material

Authors: A Bregård, P Vu, G Geitvik, A-L Børresen-Dale

Published in: Breast Cancer Research | Special Issue 1/2000

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Excerpt

Formalin-fixed, paraffin-embedded materials are an invaluable source for mutation analysis. However, DNA extraction from this type of material can be challenging as well as time consuming. DNA extracted from paraffin-embedded material is often highly fragmented and contaminated by protein agents. For DNA analysis, such as PCR (polymerase chain reaction), subsequent TTGE (temporal temperature gradient electrophoresis) and DNA sequencing, optimal conditions require long DNA fragments (200-1000 bp) and a DNA with high purity with an OD ratio (abs. at 260 nm/abs at 280 nm) between 1.6 and 2.0. We have evaluated and combined different protocols to get the highest quality and yield of DNA. 5-10 mm × 8-10 sections of tissue were used. …
Metadata
Title
Promising method for DNA extraction from paraffin embedded archive material
Authors
A Bregård
P Vu
G Geitvik
A-L Børresen-Dale
Publication date
01-03-2000
Publisher
BioMed Central
Published in
Breast Cancer Research / Issue Special Issue 1/2000
Electronic ISSN: 1465-542X
DOI
https://doi.org/10.1186/bcr173

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