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Published in:

01-09-2007 | Original

Prolonged exposure to inhaled nitric oxide does not affect haemostasis in piglets

Authors: Johanna Albert, Piotr Harbut, Stanisław Zieliński, Stanisław Ryniak, Caroline Gillis-Haegerstrand, Robert Lindwall, Leszek Solski, Jon O. Lundberg, Jan Svensson, Waldemar Goździk

Published in: Intensive Care Medicine | Issue 9/2007

Abstract

Objective

To examine possible adverse effects on haemostasis from prolonged exposure to inhaled nitric oxide (iNO).

Design and setting

Blinded, randomised, experimental animal study in a university animal laboratory.

Interventions

Anaesthetised and intubated piglets received central venous, arterial, and transabdominal urinary catheters. Twelve piglets were studied with triggered pressure support ventilation breathing with an air–oxygen mixture for 30 h with nitric oxide (NO), 40 parts per million (ppm) (n = 6) or without NO gas (n = 6) added.

The tests of platelet function were assessed in a separate 1-h experiment in which 12 additional animals were blindly randomised to receive intravenous acetylsalicylic acid (ASA) (n = 7) or placebo (n = 5).

Measurements and results

All 12 animals were clinically stable during the study period of 30 h. Haemostasis was assessed in terms of bleeding time and platelet function by Adeplat-S, reflecting platelet adhesion. Prothrombin fragment 1 + 2, fibrin D-dimer, tissue plasminogen activator and prothrombin complex were measured to investigate whether inhaled NO (iNO) had any effects on thrombin formation, fibrin formation, fibrinolysis or coagulation. All parameters including bleeding time and Adeplat-S were unaffected by iNO. ASA significantly increased bleeding time, but did not affect Adeplat-S. Nitrate in plasma and NOx (nitrate and nitrite) in urine increased significantly in pigs receiving iNO compared with controls.

Conclusions

Prolonged exposure to iNO at 40 ppm did not affect bleeding time or coagulation parameters in healthy piglets. The findings do not support the hypothesis that iNO increases the risk of bleeding in humans.

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Title: Prolonged exposure to inhaled nitric oxide does not affect haemostasis in piglets
Authors: Johanna Albert
Piotr Harbut
Stanisław Zieliński
Stanisław Ryniak
Caroline Gillis-Haegerstrand
Robert Lindwall
Leszek Solski
Jon O. Lundberg
Jan Svensson
Waldemar Goździk
Publication date: 01-09-2007
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 9/2007
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-007-0666-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Prolonged exposure to inhaled nitric oxide does not affect haemostasis in piglets

Abstract

Objective

Design and setting

Interventions

Measurements and results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Design and setting

Interventions

Measurements and results

Conclusions

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