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Archives of Gynecology and Obstetrics

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01-05-2015 | Maternal-Fetal Medicine

Proliferation of trophoblasts and Ki67 expression in preeclampsia

Authors: Barış Kaya, Umit Nayki, Cenk Nayki, Pasa Ulug, Gokalp Oner, Emre Gultekin, Yusuf Yildirim

Published in: Archives of Gynecology and Obstetrics | Issue 5/2015

Abstract

Background/aims

Preeclampsia is a pregnancy-specific disease with the increased risk of maternal morbidity and mortality. It is characterised by placental vascular dysfunction. Despite the numerous studies on preeclampsia, studies evaluating proliferation of villous trophoblasts in preeclamptic placentas are limited. Ki67 is a proliferation marker that expresses in the nuclei of proliferating cells. In this study, we examined the proliferation of villous trophoblasts in placentas of preeclamptic patients by using Ki67 and compared it with placentas of normal pregnant patients.

Material and methods

The current study is a prospective one, including 15 placentas from preeclamptic patients and 14 placentas from normal pregnancies as controls. For detection of proliferation in villous trophoblasts, Ki67 was used.

Results

The Ki67 index was 11.48±1.67% in normal patients and 15.53±2.28% in preeclamptic patients. There was a difference in Ki67 index between the two groups (p < 0.001).

Conclusion

Our results support the opinion that trophoblasts undergo regeneration hyperplasia as a result of injuries arising on the villous surface in preeclampsia. Proliferation of trophoblasts may contribute the development of preeclampsia.

Sibai BM (2005) Magnesium sulfate prophylaxis in preeclampsia: evidence from randomized trials. Clin Obstet Gynecol 48(2):478–488CrossRefPubMed

Mohaupt M (2007) Molecular aspects of preeclampsia. Mol Asp Med 28(2):169–191CrossRef

Higgins JR, de Swiet M (2001) Blood pressure measurement and classification in pregnancy. Lancet 357(9250):131–135CrossRefPubMed

Redman CWG, Sargent IL (2001) The pathogenesis of pre-eclampsia. Gynecol Obstet Fertil 29(7–8):518–522CrossRefPubMed

Granger JP, Joey P, Alexander BT, Llinas MT, Benett WA, Khalil RA (2002) Pathophysiology of preeclampsia: linking placental ischemia/hypoxia with microvascular dysfunction. Microcirculation 9(3):147–160CrossRefPubMed

Brosenes IA, Robertson WB, Dixon HG (1972) The role of spiral arteries in the pathogenesis of preeclampsia. In: Wynn RM (ed) Obstetrics and gynecology annual. Appleton-Century-Crofts, New York, pp 177–191

Madazlı R, Budak E, Calay Z, Aksu MF (2000) Correlation between placental bed biopsy findings, vascular cell adhesion molecule (VCAM-1) and fibronectin levels in preeclampsia. BJOG 107(4):514–518CrossRefPubMed

Madazlı R, Benian A, Aydın S, Uzun S, Tolun N (2002) The plasma and placental levels of malondialdehyde, glutathione and superoxide dismutase in preeclampsia. J Obstet Gynecol 22(5):477–480CrossRef

Madazlı R, Somunkiran A, Calay Z, Ilvan S, Aksu MF (2003) Histomorphology of the placenta and the placental bed of growth restricted foetuses and correlation with the Doppler velocimetries of the uterine and umbilical arteries. Placenta 24(5):510–516CrossRefPubMed

10.

Hung TH, Burton GJ (2006) Hypoxia-reoxygenation; a possible mechanism for placental oxidative stress in preeclampsia. Tawain J Obstet Gynecol 45(3):189–200CrossRef

11.

Raymond W, Redline MD, Patterson BS (1995) Pre-eclampsia is associated with an excess of proliferative immature intermediate trophoblast. Hum Pathol 26(6):594–600CrossRef

12.

Scholzen T, Gerdes J (2000) The Ki-67 protein: from the known and the unknown. J Cell Physiol 182(3):311–322CrossRefPubMed

13.

ACOG Committe on Obstetric Practice, ACOG practice bulletin (2002) Diagnosis and management of pre-eclampsia and eclampsia. In J Gynaecol Obstet 77(1):67–75

14.

Raymond D, Peterson E (2011) A critical review of early-onset and late-onset preeclampsia. Obstet Gynecol Surv 66(8):497–506CrossRefPubMed

15.

Benirschke K, Kaufmann P (1995) Basic structure of the villous tree. In: Kaufmann P (ed) Pathology of the placenta, 3rd edn. Springer Verlag, New York, pp 71–79CrossRef

16.

Simpson RA, Mayhew TM, Barnes PR (1992) From 13 weeks to term, the trophoblast of human placenta grows by continuous recruitment of new proliferative unit: a study of nuclear number using dissector. Placenta 13(5):501–502CrossRefPubMed

17.

Mochizuki M, Maruo T, Samoto T, Ishihara N (1998) Biology of human trophoblast. Int J Gynaecol Obstet 60(Suppl 1):S21–S28CrossRefPubMed

18.

Genbacev O, McMaster MT, Fischer SJ (2000) A repertoire of cell cycle regulators whose expression is coordinated with human cytotrophoblast differentiation. Am J Pathol 157:1337–1351CrossRefPubMedCentralPubMed

19.

Suresh UR, Hale RJ, Fox H, Buckley CH (1993) Use of proliferation cell nuclear antigen immunoreactivity for distinguishing hydropic abortions from partial hydatidiform moles. J Clin Pathol 46(1):48–50CrossRefPubMedCentralPubMed

20.

Arnholdt H, Meisel F, Fandrey K, Löhrs U (1991) Proliferation of villous trophoblast of the human placenta in normal and abnormal pregnancies. Virchow Archiv B Cell Pathology 60(6):365–372CrossRef

21.

Soma H, Yoshida K, Mukaida T, Tabuchi Y (1982) Morphologic changes in the hypertensive placenta. Contr Gynecol Obstet 9:58–75

22.

Teadstale F (1985) Histomorphometry of the human placenta in maternal preeclampsia. Am J Obstet Gynecol 152(1):25–31CrossRef

23.

Hustin J, Foidart JM, Lambotte R (1984) Cellular proliferation in villi of normal and pathological pregnancies. Gynecol Obstet Invest 17(1):1–9CrossRefPubMed

24.

Unek G, Ozmen A, Mendilcioglu I, Simsek M, Korgun ET (2014) Immunohistochemical distribution of cell cycle proteins p27, p57, cyclin D3, PCNA and Ki67 in normal and diabetic human placentas. J Mol Hist 45:21–34CrossRef

25.

Unek G, Ozmen A, Ozekinci M, Sakinci M, Korgun ET (2014) Immunolocalization of cell cycle proteins (p57, p27, cyclin D3, PCNA and Ki67) in intrauterine growth retardation (IUGR) and normal human term placentas. Acta Histochem 116:493–502CrossRefPubMed

26.

Jeschke U, Schiessl B, Mylonas I, Kunze S, Kuhn C, Schulze S et al (2006) Expression of the proliferation marker Ki-67 and p53 tumor protein in trophoblastic tissue of preeclamptic, HELLP, and intrauterine growth-restricted pregnancies. Int J Gynecolo Pathol 25(4):354–360CrossRef

27.

Thomas SZ, Prusac IK, Roje D, Tadin I (2011) Trophoblast apoptosis in placentae from pregnancies complicated by preeclampsia. Gynecol Obstet Invest 71(4):250–255CrossRef

28.

Prusac IK (2011) Zekic Tomas S, Roje D. Apoptosis, proliferation and Fas ligand expression in placental trophoblast from pregnancies complicated by HELLP syndrome or preeclampsia. Acta Obstet Gynecol Scand 90(10):1157–1163CrossRefPubMed

29.

Unek G, Oxmen A, Mendilcioglu I, Simsek M, Korgun ET (2014) The expression of cell cycle related proteins PCNA, Ki67, p27 and p57 in normal and preeclamptic human placentas. Tissue Cell 46:198–205CrossRefPubMed

30.

Elpek GÜ, Karaveli Ş, Keleş N (2000) Preeklampsili olguların term plasentalarında villöz trofoblast proliferasyonunun incelenmesi (Evaluation of proliferation of villous trophoblasts in term placentae of preeclamptic patients). Turkish J Pathol 16(1–2):10–12

31.

Lyall F, Myatt L (2002) The role of placenta in pre-eclampsia-a workshop report. Placenta 23:142–145CrossRef

32.

Redline RW, Patterson P (1995) Pre-eclampsia is associated with an excess of proliferative immature intermediate trophoblast. Hum Pathol 26:594–600CrossRefPubMed

33.

Kaltenbach FJ, Fettig O, Krieger ML (1974) Autoradiographische untersuchungen über das proliferationsverhalten der menschlichen placenta unter normalen und pathologischen bedingungen [Radioautographic observations on DNA-synthesis in the human placenta under normal and pathologic conditions (author’s transl). Arch Gynakol 216(4):369–386CrossRefPubMed

Title: Proliferation of trophoblasts and Ki67 expression in preeclampsia
Authors: Barış Kaya
Umit Nayki
Cenk Nayki
Pasa Ulug
Gokalp Oner
Emre Gultekin
Yusuf Yildirim
Publication date: 01-05-2015
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 5/2015
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-014-3538-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Proliferation of trophoblasts and Ki67 expression in preeclampsia

Abstract

Background/aims

Material and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background/aims

Material and methods

Results

Conclusion

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