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01-06-2013 | Original Article

Proliferation and osteogenic differentiation of osteoblast-like cells obtained from two techniques for harvesting intraoral bone grafts

Authors: Francisco Javier Manzano-Moreno, Juan Bautista Rodríguez-Martínez, Javier Ramos-Torrecillas, Manuel Francisco Vallecillo-Capilla, Concepción Ruiz, Olga García-Martínez, Candela Reyes-Botella

Published in: Clinical Oral Investigations | Issue 5/2013

Abstract

Objectives

The aims of our study were to verify the presence of viable osteoblasts in samples of bone tissue obtained by drilling or from cortico-cancellous bone blocks and to assess their growth and differentiation capacities.

Materials and methods

Bone tissue samples were processed independently and cultured in Dulbecco’s modified Eagle medium, in a CO₂ incubator at 37 °C. The proliferative capacity of osteoblasts was determined by spectrophotometry (MTT) at 24 and 48 h of culture. Cell cycle was analysed by flow cytometry. Cell differentiation was studied by red alizarin staining of nodules formed in mineralisation medium and by analysis of alkaline phosphatase activity.

Results

In comparison to bone block-derived osteoblasts, the proliferative capacity was greater at 24 and 48 h of culture (P < 0.001) in the drilling-derived osteoblasts, which showed significantly increased G2/M (P = 0.014) and S (P < 0.001) phases in the cell cycle study. The number of mineralised nodules was proportional to the incubation time, with no differences between the two types of sample, which also did not significantly differ in alkaline phosphatase activity.

Conclusion

Superior autograft material is obtained by harvesting particulate bone from low-speed drilling fragments than from a cortico-cancellous bone block.

Clinical relevance

These results suggest that bone obtained from low-speed drilling is a simple and effective alternative to the classic procedure for obtaining bone tissue.

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Title: Proliferation and osteogenic differentiation of osteoblast-like cells obtained from two techniques for harvesting intraoral bone grafts
Authors: Francisco Javier Manzano-Moreno
Juan Bautista Rodríguez-Martínez
Javier Ramos-Torrecillas
Manuel Francisco Vallecillo-Capilla
Concepción Ruiz
Olga García-Martínez
Candela Reyes-Botella
Publication date: 01-06-2013
Publisher: Springer-Verlag
Published in: Clinical Oral Investigations / Issue 5/2013
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-012-0811-4

At a glance: The STEP trials

Springer Medicine

Proliferation and osteogenic differentiation of osteoblast-like cells obtained from two techniques for harvesting intraoral bone grafts

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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