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Journal of Orthopaedic Surgery and Research
Published in: Journal of Orthopaedic Surgery and Research 1/2007

Open Access 01-12-2007 | Research article

Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells

Authors: Britt Wildemann, Nicole Burkhardt, Marc Luebberstedt, Thomas Vordemvenne, Gerhard Schmidmaier

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2007

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Abstract

Growth factors are in clinical use to stimulate bone growth and regeneration. BMP-2 is used in long bone and spinal surgery, PDGFbb for the treatment of periodontal defects and children with growth hormone receptor deficiency are treated with IGF-I.
Aim of the present study was the comparative analysis of the effect of these growth factors released from a local drug delivery system on cells of the osteogenic lineage at differing differentiation stages.
The experiments with the mesenchymal cell line C2C12 revealed a proliferating effect of all three growth factors and a differentiating effect of BMP-2 with a dramatic increase in alkaline phosphatase activity. None of the growth factors stimulated cell migration.
Human osteoblast like cells showed similar results with an increase in proliferation after stimulation with IGF-I or PDGFbb. The enzymatic activity of alkaline phosphatase was enhanced only in the cells stimulated with BMP-2. This group showed also more mineralized matrix compared to the other groups.
In conclusion, the growth factors IGF-I and PDGFbb delivered with a local drug delivery system stimulated cell proliferation, whereas BMP-2 showed a dramatic effect on differentiation on osteoblast precursor cells and osteoblast like cells.
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Metadata
Title
Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells
Authors
Britt Wildemann
Nicole Burkhardt
Marc Luebberstedt
Thomas Vordemvenne
Gerhard Schmidmaier
Publication date
01-12-2007
Publisher
BioMed Central
Published in
Journal of Orthopaedic Surgery and Research / Issue 1/2007
Electronic ISSN: 1749-799X
DOI
https://doi.org/10.1186/1749-799X-2-27

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