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International Journal of Colorectal Disease

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01-11-2016 | Original Article

Preoperative vitamin C supplementation improves colorectal anastomotic healing and biochemical parameters in malnourished rats

Authors: Lucineia Gainski Danielski, Eduardo Walczewski, Clovisa Reck de Jesus, Drielly Florentino, Amanda Della Giustina, Mariana Pereira Goldim, Luiz Alberto Kanis, Gregório W. Pereira, Volnei D. Pereira, Francine Felisberto, Fabricia Petronilho

Published in: International Journal of Colorectal Disease | Issue 11/2016

Abstract

Purposes

The objective of this study was to evaluate the effect of supplementation with vitamin C on intestinal anastomosis healing in malnourished rats.

Methods

Male Wistar rats were divided into three groups: (1) sham, well-nourished rats that received vehicle; (2) FR+Veh, rats that were subjected to food restriction and received vehicle; and (3) FR+VC, rats that were subjected to food restriction and received vitamin C. Four days before surgery, the animals received vitamin C (100 mg/kg/day) via gavage and underwent colon resection with anastomosis in a single plane. The survival rate of rats was monitored until day 7 after surgery. Regarding anastomosis tissues, we examined intra-abdominal adhesion index, hydroxyproline content, collagen density, inflammatory parameters, and oxidative damage to proteins and lipids.

Results

Malnutrition decreases body weight and increases mortality; the survival rate was 90 % in group 1, 60 % in group 2, and 80 % in group 3. Vitamin C was able to increase hydroxyproline concentration and density of collagen and decrease the intra-abdominal adhesion index, as well as the infiltration of neutrophils and oxidative damage to proteins in malnourished rats compared to group treated with vehicle.

Conclusions

Preoperative vitamin C supplementation can improve the intestinal anastomosis healing, biochemical alterations, and prolong survival in rats subjected to food restriction.

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Title: Preoperative vitamin C supplementation improves colorectal anastomotic healing and biochemical parameters in malnourished rats
Authors: Lucineia Gainski Danielski
Eduardo Walczewski
Clovisa Reck de Jesus
Drielly Florentino
Amanda Della Giustina
Mariana Pereira Goldim
Luiz Alberto Kanis
Gregório W. Pereira
Volnei D. Pereira
Francine Felisberto
Fabricia Petronilho
Publication date: 01-11-2016
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Colorectal Disease / Issue 11/2016
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-016-2647-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Preoperative vitamin C supplementation improves colorectal anastomotic healing and biochemical parameters in malnourished rats

Abstract

Purposes

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purposes

Methods

Results

Conclusions

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