Top

World Journal of Surgery

Published in:

01-02-2011

Preoperative Toll-like Receptor Expression in Monocytes is Associated with Host Immunological Responses Following Gastrointestinal Surgery

Authors: Satoshi Ono, Hironori Tsujimoto, Shu-ichi Hiraki, Manabu Kinoshita, Kazuo Hase

Published in: World Journal of Surgery | Issue 2/2011

Abstract

Background

The present study was designed to evaluate the relationship between preoperative Toll-like receptor (TLR) expression in peripheral blood monocytes (PBMC) and the postoperative inflammatory cytokine secretion from PBMC, as well as the postoperative infectious complications in patients with gastrointestinal cancer.

Methods

Peripheral blood monocytes were isolated from 26 patients on the preoperative day and on postoperative day 1 (POD1). The monocytes were stained for CD14, TLR2, and TLR4 surface expression and stimulated ex vivo with lipopolysaccharide (LPS), after which the culture supernatant tumor necrosis factor alpha (TNFα), interleukin (IL)-1β, and interferon gamma (IFNγ) concentrations were measured in the culture supernatant. Postoperative clinical courses were monitored.

Results

There was a significant positive correlation between preoperative TLR4 expression and clinical stage in patients with gastrointestinal cancer. A significant negative correlation between preoperative TLR2 or TLR4 expression and the preoperative TNFα and IL-1β production was found. Furthermore, there was a significant negative correlation between preoperative TLR2 or TLR4 expression and postoperative inflammatory cytokine production.

Conclusions

Preoperative high expression of TLR on monocytes reduces the capacity for LPS-induced inflammatory cytokine production from monocytes. An analysis of preoperative TLR expression may therefore help to elucidate the systemic immunological response and the clinical course following gastrointestinal surgery.

Aosasa S, Ono S, Mochizuki H et al (2000) Activation of monocytes and endothelial cells depends on the severity of surgical stress. World J Surg 24:10–16CrossRefPubMed

Ikuta S, Ono S, Kinoshita M et al (2003) Interleukin-18 concentration in the peritoneal fluid correlates with the severity of peritonitis. Am J Surg 185:550–555CrossRefPubMed

Bone RC (1996) Toward a theory regarding the pathogenesis of the systemic inflammatory response syndrome: what we do and do not know about cytokine regulation. Crit Care Med 24:163–172CrossRefPubMed

Wang SY, Mak KL, Chen LY et al (1992) Heterogeneity of human blood monocyte: two subpopulations with different sizes, phenotypes and functions. Immunology 77:298–303PubMed

Fingerle-Rowson G, Angstwurm M, Andreesen R et al (1998) Selective depletion of CD14+ CD16+ monocytes by glucocorticoid therapy. Clin Exp Immunol 112:501–506CrossRefPubMed

Medzhitov R, Janeway CA Jr (1997) Innate immunity: the virtues of a nonclonal system of recognition. Cell 91:295–298CrossRefPubMed

Ulevitch RJ, Tobias PS (1995) Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu Rev Immunol 13:437–457CrossRefPubMed

Takeshita F, Leifer CA, Gursel I et al (2001) Cutting edge: role of Toll-like receptor 9 in CpG DNA-induced activation of human cells. J Immunol 167:3555–3558PubMed

Schwandner R, Dziarski R, Wesche H et al (1999) Peptidoglycan- and lipoteichoic acid-induced cell activation is mediated by toll-like receptor 2. J Biol Chem 274:17406–17409CrossRefPubMed

10.

Yang H, Young DW, Gusovsky F et al (2000) Cellular events mediated by lipopolysaccharide-stimulated Toll-like receptor 4. MD-2 is required for activation of mitogen-activated protein kinases and Elk-1. J Biol Chem 275:20861–20866CrossRefPubMed

11.

Arbour NC, Lorenz E, Schutte BC et al (2000) TLR4 mutations are associated with endotoxin hyporesponsiveness in humans. Nat Genet 25:187–191CrossRefPubMed

12.

Park JS, Svetkauskaite D, He Q et al (2004) Involvement of Toll-like receptors 2 and 4 in cellular activation by high mobility group box 1 protein. J Biol Chem 279:7370–7377CrossRefPubMed

13.

Sasu S, LaVerda D, Qureshi N et al (2001) Chlamydia pneumoniae and chlamydial heat shock protein 60 stimulate proliferation of human vascular smooth muscle cells via toll-like receptor 4 and p44/p42 mitogen-activated protein kinase activation. Circ Res 89:244–250CrossRefPubMed

14.

Devaney JM, Greene CM, Taggart CC et al (2003) Neutrophil elastase up-regulates interleukin-8 via toll-like receptor 4. FEBS Lett 544:129–132CrossRefPubMed

15.

Smiley ST, King JA, Hancock WW (2001) Fibrinogen stimulates macrophage chemokine secretion through toll-like receptor 4. J Immunol 167:2887–2894PubMed

16.

Sobin LH, Fleming ID (1997) TNM classification of malignant tumors, fifth edition, Union Internationale Contre le Cancer and the American Joint Committee on Cancer. Cancer 80:1803–1804CrossRefPubMed

17.

Hoffmann JA, Kafatos FC, Janeway CA et al (1999) Phylogenetic perspective in innate immunity. Science 284:1313–1318CrossRefPubMed

18.

Takeda K, Kaisho T, Akira S (2003) Toll-like receptors. Annu Rev Immunol 21:335–376CrossRefPubMed

19.

Chow JC, Young DW, Golenbock DT et al (1999) Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. J Biol Chem 274:10689–10692CrossRefPubMed

20.

Lein E, Sellati TJ, Yoshimura A et al (1999) Toll-like receptor-2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem 274:33419–33425CrossRef

21.

Dziarski R, Wang O, Miyake K et al (2001) MD-2 enables Toll-like receptor 2 mediated response to lipopolysaccharide and enhances TLR-2 mediated responses to Gram-positive and Gram-negative bacteria and their cell wall components. J Immunol 166:1938–1944PubMed

22.

Tsujimoto H, Ono S, Majima T et al (2006) Differential Toll-like receptor expression after ex vivo lipopolysaccharide exposure in patients with sepsis and following surgical stress. Clin Immunol 119:180–187CrossRefPubMed

23.

Tsujimoto H, Ono S, Efron PA et al (2008) Role of Toll-like receptor in the development of sepsis. Shock 29:315–321PubMed

24.

McCall CE, Grosso-Wilmoth LM, LaRue K et al (1993) Tolerance to endotoxin-induced expression of the interleukin-1 beta gene in blood neutrophils of humans with the sepsis syndrome. J Clin Invest 91:853–861CrossRefPubMed

25.

Calvano JE, Agnese DM, Um JY et al (2003) Modulation of the lipopolysaccharide receptor complex (CD14, TLR4, MD-2) and Toll-like receptor 2 in systemic inflammatory response syndrome-positive patients with and without infection: relationship to tolerance. Shock 20:415–419CrossRefPubMed

26.

Armstrong L, Medford AR, Hunter KJ et al (2004) Differential expression of Toll-like receptor (TLR)-2 and TLR-4 on monocytes in human sepsis. Clin Exp Immunol 136:312–319CrossRefPubMed

Title: Preoperative Toll-like Receptor Expression in Monocytes is Associated with Host Immunological Responses Following Gastrointestinal Surgery
Authors: Satoshi Ono
Hironori Tsujimoto
Shu-ichi Hiraki
Manabu Kinoshita
Kazuo Hase
Publication date: 01-02-2011
Publisher: Springer-Verlag
Published in: World Journal of Surgery / Issue 2/2011
Print ISSN: 0364-2313
Electronic ISSN: 1432-2323
DOI: https://doi.org/10.1007/s00268-010-0839-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Preoperative Toll-like Receptor Expression in Monocytes is Associated with Host Immunological Responses Following Gastrointestinal Surgery

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 2/2011

Preliminary Report: Search for a Transgastric Approach for Managing Gastrogastric Fistulas

Analysis of the Clinicopathologic Features of Papillary Thyroid Microcarcinoma Based on 7-mm Tumor Size

Computed Tomography Volumetric Fat Parameters versus Body Mass Index for Predicting Short-term Outcomes of Colon Surgery

Challenges of Meeting Surgical Needs in the Developing World

Erratum to: Establishment of an Intraoperative Staging System (iStage) by Improving UICC TNM Classification System for Papillary Thyroid Carcinoma

Donor Conversion and Procurement Failure: The Fate of Our Potential Organ Donors