Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Diseases of the Colon & Rectum
Published in: Diseases of the Colon & Rectum 7/2007

01-07-2007

Prebiotic and Synbiotic Fructooligosaccharide Administration Fails to Reduce the Severity of Experimental Colitis in Rats

Authors: Mark S. Geier, B.Sc.(Hons.), Ross N. Butler, Ph.D., Philip M. Giffard, Ph.D., Gordon S. Howarth, Ph.D.

Published in: Diseases of the Colon & Rectum | Issue 7/2007

Login to get access

Abstract

Opposing effects of the prebiotic, fructooligosaccharide, have been reported in experimental colitis. We compared the effects of the prebiotic, fructooligosaccharide, alone and in synbiotic combination with Lactobacillus fermentum BR11, on the development of dextran sulfate sodium-induced colitis in rats. Rats consumed an 18 percent casein-based diet or diet supplemented with 6 percent fructooligosaccharide or maltodextrin for 14 days. The synbiotic group was gavaged 1 ml of L. fermentum BR11 (1 × 109 cfu/ml) twice daily. From Days 7 to 14, colitis was induced via 3 percent dextran sulfate sodium in drinking water. Disease activity was assessed daily, and at killing, gastrointestinal organs were measured, weighed, and examined by quantitative histology, proliferating cell nuclear antigen immunohistochemistry, and colonic myeloperoxidase activity. Administration of dextran sulfate sodium resulted in an increased colitic disease activity, and an increased colon and cecum weight compared with normal controls. Colon and cecum weights were further increased in dextran sulfate sodium+fructooligosaccharide (colon: 19 percent; cecum: 48 percent) and dextran sulfate sodium+fructooligosaccharide/L. fermentum BR11-treated rats (16 and 62 percent) compared with dextran sulfate sodium+vehicle-treatment. Dextran sulfate sodium+fructooligosaccharide-treated rats displayed an 81 percent increase in colonic myeloperoxidase activity compared with dextran sulfate sodium-treated controls. Histologic damage severity scores increased in dextran sulfate sodium+vehicle, dextran sulfate sodium+fructooligosaccharide, and dextran sulfate sodium+fructooligosaccharide/L. fermentum BR11-treated rats compared with normal controls (P < 0.05). Crypt depth increased in all treatments compared with normal controls (P < 0.01). No protection from dextran sulfate sodium-colitis was accorded by fructooligosaccharide alone or in synbiotic combination with L. fermentum BR11, whereas fructooligosaccharide actually increased some indicators of colonic injury.
Literature
1.
Gaya, DR, Russell, RK, Nimmo, ER, Satsangi, J 2006New genes in inflammatory bowel disease: lessons for complex diseases?Lancet36712711284PubMedCrossRef
2.
Hendrickson, BA, Gokhale, R, Cho, JH 2002Clinical aspects and pathophysiology of inflammatory bowel diseaseClin Microbiol Rev157994PubMedCrossRef
3.
Thompson-Chagoyan, OC, Maldonado, J, Gil, A 2005Aetiology of inflammatory bowel disease (IBD): role of intestinal microbiota and gut-associated lymphoid tissue immune response.Clin Nutr24339352PubMedCrossRef
4.
Madsen, KL, Doyle, JS, Jewell, LD, Tavernini, MM, Fedorak, RN 1999Lactobacillus species prevents colitis in interleukin 10 gene-deficient mice.Gastroenterology11611071114PubMedCrossRef
5.
Osman, N, Adawi, D, Ahrne, S, Jeppsson, B, Molin, G 2004Modulation of the effect of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium.Dig Dis Sci49320327PubMedCrossRef
6.
Dieleman, LA, Goerres, MS, Arends, A,  et al. 2003Lactobacillus GG prevents recurrence of colitis in HLA-B27 transgenic rats after antibiotic treatment.Gut52370376PubMedCrossRef
7.
Moller, PL, Paerregaard, A, Gad, M, Kristensen, NN, Claesson, MH 2005Colitic scid mice fed Lactobacillus spp. show an ameliorated gut histopathology and an altered cytokine profile by local T cells.Inflamm Bowel Dis11814819PubMedCrossRef
8.
Rachmilewitz, D, Katakura, K, Karmeli, F,  et al. 2004Toll-like receptor 9 signaling mediates the anti-inflammatory effects of probiotics in murine experimental colitis.Gastroenterology126520528PubMedCrossRef
9.
Mimura, T, Rizzello, F, Helwig, U,  et al. 2004Once daily high dose probiotic therapy (VSL#3) for maintaining remission in recurrent or refractory pouchitis.Gut53108114PubMedCrossRef
10.
Gionchetti, P, Rizzello, F, Helwig, U,  et al. 2003Prophylaxis of pouchitis onset with probiotic therapy: a double-blind, placebo-controlled trial.Gastroenterology12412021209PubMedCrossRef
11.
Gionchetti, P, Rizzello, F, Venturi, A,  et al. 2000Oral bacteriotherapy as maintenance treatment in patients with chronic pouchitis: a double-blind, placebo-controlled trial.Gastroenterology119305309PubMedCrossRef
12.
Bibiloni, R, Fedorak, RN, Tannock, GW,  et al. 2005VSL#3 probiotic-mixture induces remission in patients with active ulcerative colitis.Am J Gastroenterol10015391546PubMedCrossRef
13.
Rembacken, BJ, Snelling, AM, Hawkey, PM, Chalmers, DM, Axon, AT 1999Non-pathogenic Escherichia coli versus mesalazine for the treatment of ulcerative colitis: a randomised trial.Lancet354635639PubMedCrossRef
14.
Kruis, W, Fric, P, Pokrotnieks, J,  et al. 2004Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine.Gut5316171623PubMedCrossRef
15.
Rush, CM, Hafner, LM, Timms, P 1994Genetic modification of a vaginal strain of Lactobacillus fermentum and its maintenance within the reproductive tract after intravaginal administration.J Med Microbiol41272278PubMedCrossRef
16.
Hung, J, Turner, MS, Walsh, T, Giffard, PM 2005BspA (CyuC) in Lactobacillus fermentum BR11 is a highly expressed high-affinity L-cystine-binding protein.Curr Microbiol503337PubMedCrossRef
17.
Hung, J, Cooper, D, Turner, MS, Walsh, T, Giffard, PM 2003Cystine uptake prevents production of hydrogen peroxide by Lactobacillus fermentum BR11.FEMS Microbiol Lett2279399PubMedCrossRef
18.
Turner, MS, Woodberry, T, Hafner, LM, Giffard, PM 1999The bspA locus of Lactobacillus fermentum BR11 encodes an L-cystine uptake system.J Bacteriol18121922198PubMed
19.
Geier MS, Butler RN, Giffard PM, Howarth GS. Lactobacillus fermentum BR11, a potential new probiotic, alleviates symptoms of colitis induced by dextran sulfate sodium (DSS) in rats. Int J Food Microbiol 2006;[Epub ahead of print]
20.
Bengmark, S 2002Gut microbial ecology in critical illness: is there a role for prebiotics, probiotics, and synbiotics?Curr Opin Crit Care8145151PubMedCrossRef
21.
Fukuda, M, Kanauchi, O, Araki, Y,  et al. 2002Prebiotic treatment of experimental colitis with germinated barley foodstuff: a comparison with probiotic or antibiotic treatment.Int J Mol Med96570PubMed
22.
Rumi, G, Tsubouchi, R, Okayama, M, Kato, S, Mozsik, G, Takeuchi, K 2004Protective effect of lactulose on dextran sulfate sodium-induced colonic inflammation in rats.Dig Dis Sci4914661472PubMedCrossRef
23.
Welters, CF, Heineman, E, Thunnissen, FB, Bogaard, AE, Soeters, PB, Baeten, CG 2002Effect of dietary inulin supplementation on inflammation of pouch mucosa in patients with an ileal pouch-anal anastomosisDis Colon Rectum45621627PubMedCrossRef
24.
Bomba, A, Nemcova, R, Gancarcikova, S, Herich, R, Guba, P, Mudronova, D 2002Improvement of the probiotic effect of micro-organisms by their combination with maltodextrins, fructo-oligosaccharides and polyunsaturated fatty acids.Br J Nutr88S95S99PubMedCrossRef
25.
Ten Bruggencate, SJ, Bovee-Oudenhoven, IM, Lettink-Wissink, ML, Meer, R 2005Dietary fructooligosaccharides increase intestinal permeability in rats.J Nutr135837842PubMed
26.
Furrie, E, Macfarlane, S, Kennedy, A,  et al. 2005Synbiotic therapy (Bifidobacterium longum/Synergy 1) initiates resolution of inflammation in patients with active ulcerative colitis: a randomised controlled pilot trial.Gut54242249PubMedCrossRef
27.
Tomas, FM, Knowles, SE, Owens, PC,  et al. 1991Increased weight gain, nitrogen retention and muscle protein synthesis following treatment of diabetic rats with insulin-like growth factor (IGF)-I and des(1-3)IGF-I.Biochem J276547554PubMed
28.
Murthy, SN, Cooper, HS, Shim, H, Shah, RS, Ibrahim, SA, Sedergran, DJ 1993Treatment of dextran sulfate sodium-induced murine colitis by intracolonic cyclosporin.Dig Dis Sci3817221734PubMedCrossRef
29.
Geier, MS, Tenikoff, D, Yazbeck, R, McCaughan, GW, Abbott, CA, Howarth, GS 2005Development and resolution of experimental colitis in mice with targeted deletion of dipeptidyl peptidase IV.J Cell Physiol204687692PubMedCrossRef
30.
Howarth, GS, Xian, CJ, Read, LC 2000Predisposition to colonic dysplasia is unaffected by continuous administration of insulin-like growth factor-I for twenty weeks in a rat model of chronic inflammatory bowel disease.Growth Factors18119133PubMedCrossRef
31.
Krawisz, JE, Sharon, P, Stenson, WF 1984Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models.Gastroenterology8713441350PubMed
32.
Schultz, M, Timmer, A, Herfarth, HH, Sartor, RB, Vanderhoof, JA, Rath, HC 2004Lactobacillus GG in inducing and maintaining remission of Crohn’s diseaseBMC. Gastroenterol45PubMedCrossRef
33.
Cherbut, C, Michel, C, Lecannu, G 2003The prebiotic characteristics of fructooligosaccharides are necessary for reduction of TNBS-induced colitis in rats.J Nutr1332127PubMed
34.
Moreau, NM, Martin, LJ, Toquet, CS,  et al. 2003Restoration of the integrity of rat caeco-colonic mucosa by resistant starch, but not by fructo-oligosaccharides, in dextran sulfate sodium-induced experimental colitis.Br J Nutr907585PubMedCrossRef
35.
Ten Bruggencate, SJ, Bovee-Oudenhoven, IM, Lettink-Wissink, ML, Katan, MB, Meer, R 2004Dietary fructo-oligosaccharides and inulin decrease resistance of rats to salmonella: protective role of calcium.Gut53530535PubMedCrossRef
36.
Ten Bruggencate, SJ, Bovee-Oudenhoven, IM, Lettink-Wissink, ML, Meer, R 2003Dietary fructo-oligosaccharides dose-dependently increase translocation of salmonella in rats.J Nutr13323132318PubMed
37.
Bovee-Oudenhoven, IM, ten Bruggencate, SJ, Lettink-Wissink, ML, Meer, R 2003Dietary fructo-oligosaccharides and lactulose inhibit intestinal colonisation but stimulate translocation of salmonella in rats.Gut5215721578PubMedCrossRef
38.
Campbell, JM, Fahey, GC,Jr, Wolf, BW 1997Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and microflora in rats.J Nutr127130136PubMed
39.
Burns, AJ, Rowland, IR 2004Antigenotoxicity of probiotics and prebiotics on faecal water-induced DNA damage in human colon adenocarcinoma cells.Mutat Res551233243PubMed
Metadata
Title
Prebiotic and Synbiotic Fructooligosaccharide Administration Fails to Reduce the Severity of Experimental Colitis in Rats
Authors
Mark S. Geier, B.Sc.(Hons.)
Ross N. Butler, Ph.D.
Philip M. Giffard, Ph.D.
Gordon S. Howarth, Ph.D.
Publication date
01-07-2007
Publisher
Springer-Verlag
Published in
Diseases of the Colon & Rectum / Issue 7/2007
Print ISSN: 0012-3706
Electronic ISSN: 1530-0358
DOI
https://doi.org/10.1007/s10350-007-0213-x

Other articles of this Issue 7/2007

Diseases of the Colon & Rectum 7/2007 Go to the issue

OriginalPaper

Melanoma of the Anal Canal: A Case Series

OriginalPaper

Combining Radioisotopic and Blue-Dye Technique Does Not Improve the False-Negative Rate in Sentinel Lymph Node Mapping for Colorectal Cancer

Colorectal Website Review

Inflammatory Bowel Disease

Announcement

Announcements

Report

Endobronchial Metastases from Colon Cancer without Liver Metastases: Report of Eight Cases

OriginalPaper

Detection of Metachronous Neoplasms in Colorectal Cancer Patients: Identification of Risk Factors