Top

Published in:

01-12-2016 | Original Article

Pre-coating deproteinized bovine bone mineral (DBBM) with bone-conditioned medium (BCM) improves osteoblast migration, adhesion, and differentiation in vitro

Authors: Jordi Caballé-Serrano, Masako Fujioka-Kobayashi, Dieter D. Bosshardt, Reinhard Gruber, Daniel Buser, Richard J. Miron

Published in: Clinical Oral Investigations | Issue 9/2016

Abstract

Objectives

Autogenous bone grafting has remained the gold standard for bone augmentation procedures with ability to release growth factors to the surrounding microenvironment. Recent investigations have characterized these specific growth factors released by autogenous bone chips with further isolation into a “bone-conditioned medium” (BCM). The aim of the present investigation was to utilize autologous growth factors from bone chips (BCM) in combination with deproteinized bovine bone mineral (DBBM) and investigate the ability for BCM to enhance osteoblast behavior.

Materials and methods

Mouse ST2 cells were seeded on (1) DBBM particles alone or (2) DBBM + BCM. Thereafter, samples were compared for cell recruitment, adhesion, proliferation, and real-time PCR for osteoblast differentiation markers including Runx2, collagen 1 alpha 2 (COL1A2), alkaline phosphatase (ALP), and osteocalcin (OCN). Alizarin red staining was used to assess mineralization.

Results

Coating BCM on DBBM particles improved cell migration of ST2 cells and significantly enhanced a 2-fold increase in cell adhesion. While no significant increase in cell proliferation was observed, BCM significantly increased mRNA levels of COL1A2, ALP, and OCN at 3 days post seeding. Furthermore, a 3-fold increase in alizarin red staining was observed on DBBM particles pre-coated with BCM.

Conclusion

Pre-coating DBBM with BCM enhanced the osteoconductive properties of DBBM by mediating osteoblast recruitment, attachment, and differentiation towards bone-forming osteoblasts. Future animal study is necessary to further characterize the added benefit of BCM as an autogenous growth factor source for combination therapies.

Clinical relevance

The application of BCM in combination with biomaterials may serve as an autogenous growth factor source for bone regeneration.

Stern A, Barzani G (2015) Autogenous bone harvest for implant reconstruction. Dent Clin N Am 59:409–420. doi:10.1016/j.cden.2014.10.011 CrossRefPubMed

Miron RJ, Hedbom E, Saulacic N, Zhang Y, Sculean A, Bosshardt DD, Buser D (2011) Osteogenic potential of autogenous bone grafts harvested with four different surgical techniques. J Dent Res 90:1428–1433. doi:10.1177/0022034511422718 CrossRefPubMed

Jensen SS, Bosshardt DD, Gruber R, Buser D (2014) Long-term stability of contour augmentation in the esthetic zone: histologic and histomorphometric evaluation of 12 human biopsies 14 to 80 months after augmentation. J Periodontol 85:1549–1556. doi:10.1902/jop.2014.140182 CrossRefPubMed

Buser D, Chappuis V, Kuchler U, Bornstein MM, Wittneben JG, Buser R, Cavusoglu Y, Belser UC (2013) Long-term stability of early implant placement with contour augmentation. J Dent Res 92:176s–182s. doi:10.1177/0022034513504949 CrossRefPubMedPubMedCentral

Miron RJ, Gruber R, Hedbom E, Saulacic N, Zhang Y, Sculean A, Bosshardt DD, Buser D (2013) Impact of bone harvesting techniques on cell viability and the release of growth factors of autografts. Clin Implant Dent Relat Res 15:481–489. doi:10.1111/j.1708-8208.2012.00440.x CrossRefPubMed

Brolese E, Buser D, Kuchler U, Schaller B, Gruber R (2014) Human bone chips release of sclerostin and FGF-23 into the culture medium: an in vitro pilot study. Clin Oral Implants Res. doi:10.1111/clr.12432 PubMed

Caballe-Serrano J, Bosshardt DD, Buser D, Gruber R (2014) Proteomic analysis of porcine bone-conditioned medium. Int J Oral Maxillofac Implants 29:1208–115d. doi:10.11607/jomi.3708 CrossRefPubMed

Caballe-Serrano J, Schuldt Filho G, Bosshardt DD, Gargallo-Albiol J, Buser D, Gruber R (2015) Conditioned medium from fresh and demineralized bone enhances osteoclastogenesis in murine bone marrow cultures. Clin Oral Implants Res. doi:10.1111/clr.12573 PubMed

Sawada K, Caballe-Serrano J, Schuldt Filho G, Bosshardt DD, Schaller B, Buser D, Gruber R (2015) Thermal processing of bone: in vitro response of mesenchymal cells to bone-conditioned medium. Int J Oral Maxillofac Surg. doi:10.1016/j.ijom.2015.03.012 PubMed

10.

Schuldt Filho G, Caballe-Serrano J, Sawada K, Bosshardt DD, Aurelio Bianchini M, Buser D and Gruber R (2015) Conditioned medium of demineralized freeze-dried bone activates gene expression in periodontal fibroblasts in vitro. J Periodontol:1–14. doi: 10.1902/jop.2015.140676

11.

Peng J, Nemec M, Brolese E, Bosshardt DD, Schaller B, Buser D, Gruber R (2014) Bone-conditioned medium Inhibits osteogenic and adipogenic differentiation of mesenchymal cells in vitro. Clin Implant Dent Relat Res. doi:10.1111/cid.12200 PubMed

12.

Filho GS, Caballe-Serrano J, Sawada K, Bosshardt DD, Bianchini MA, Buser D, Gruber R (2015) Conditioned medium of demineralized freeze-dried bone activates gene expression in periodontal fibroblasts in vitro. J Periodontol 86:827–834. doi:10.1902/jop.2015.140676 CrossRefPubMed

13.

Zimmermann M, Caballe-Serrano J, Bosshardt DD, Ankersmit HJ, Buser D, Gruber R (2015) Bone-conditioned medium changes gene expression in bone-derived fibroblasts. Int J Oral Maxillofac Implants 30:953–958. doi:10.11607/jomi.4060 CrossRefPubMed

14.

Miron RJ, Bosshardt DD, Hedbom E, Zhang Y, Haenni B, Buser D, Sculean A (2012) Adsorption of enamel matrix proteins to a bovine-derived bone grafting material and its regulation of cell adhesion, proliferation, and differentiation. J Periodontol 83:936–947. doi:10.1902/jop.2011.110480 CrossRefPubMed

15.

Miron RJ, Bosshardt DD, Zhang Y, Buser D, Sculean A (2013) Gene array of primary human osteoblasts exposed to enamel matrix derivative in combination with a natural bone mineral. Clin Oral Investig 17:405–410. doi:10.1007/s00784-012-0742-0 CrossRefPubMed

16.

Miron RJ, Saulacic N, Buser D, Iizuka T, Sculean A (2013) Osteoblast proliferation and differentiation on a barrier membrane in combination with BMP2 and TGFbeta1. Clin Oral Investig 17:981–988. doi:10.1007/s00784-012-0764-7 CrossRefPubMed

17.

Jung RE, Glauser R, Scharer P, Hammerle CH, Sailer HF, Weber FE (2003) Effect of rhBMP-2 on guided bone regeneration in humans. Clin Oral Implants Res 14:556–568CrossRefPubMed

18.

Wikesjo UM, Qahash M, Thomson RC, Cook AD, Rohrer MD, Wozney JM, Hardwick WR (2003) Space-providing expanded polytetrafluoroethylene devices define alveolar augmentation at dental implants induced by recombinant human bone morphogenetic protein 2 in an absorbable collagen sponge carrier. Clin Implant Dent Relat Res 5:112–123CrossRefPubMed

19.

Donos N, Glavind L, Karring T, Sculean A (2004) Clinical evaluation of an enamel matrix derivative and a bioresorbable membrane in the treatment of degree III mandibular furcation involvement: a series of nine patients. Int J Periodontics Restorative Dent 24:362–369PubMed

20.

Nevins M, Camelo M, Nevins ML, Schenk RK, Lynch SE (2003) Periodontal regeneration in humans using recombinant human platelet-derived growth factor-BB (rhPDGF-BB) and allogenic bone. J Periodontol 74:1282–1292. doi:10.1902/jop.2003.74.9.1282 CrossRefPubMed

21.

Kitamura M, Akamatsu M, Machigashira M, Hara Y, Sakagami R, Hirofuji T, Hamachi T, Maeda K, Yokota M, Kido J, Nagata T, Kurihara H, Takashiba S, Sibutani T, Fukuda M, Noguchi T, Yamazaki K, Yoshie H, Ioroi K, Arai T, Nakagawa T, Ito K, Oda S, Izumi Y, Ogata Y, Yamada S, Shimauchi H, Kunimatsu K, Kawanami M, Fujii T, Furuichi Y, Furuuchi T, Sasano T, Imai E, Omae M, Yamada S, Watanuki M, Murakami S (2011) FGF-2 stimulates periodontal regeneration: results of a multi-center randomized clinical trial. J Dent Res 90:35–40. doi:10.1177/0022034510384616 CrossRefPubMed

22.

Anusaksathien O, Giannobile WV (2002) Growth factor delivery to re-engineer periodontal tissues. Curr Pharm Biotechnol 3:129–139CrossRefPubMed

23.

Carreira AC, Lojudice FH, Halcsik E, Navarro RD, Sogayar MC, Granjeiro JM (2014) Bone morphogenetic proteins: facts, challenges, and future perspectives. J Dent Res 93:335–345. doi:10.1177/0022034513518561 CrossRefPubMed

24.

Rocque BG, Kelly MP, Miller JH, Li Y, Anderson PA (2014) Bone morphogenetic protein-associated complications in pediatric spinal fusion in the early postoperative period: an analysis of 4658 patients and review of the literature. J Neurosurg Pediatr 14:635–643. doi:10.3171/2014.8.peds13665 CrossRefPubMed

25.

Marx RE (2004) Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg: Official Journal of the American Association of Oral and Maxillofacial Surgeons 62:489–496CrossRef

26.

Borie E, Olivi DG, Orsi IA, Garlet K, Weber B, Beltran V, Fuentes R (2015) Platelet-rich fibrin application in dentistry: a literature review. Int J Clin Exp Med 8:7922–7929PubMedPubMedCentral

27.

Zimmermann M, Caballe-Serrano J, Bosshardt DD, Ankersmit HJ, Buser D and Gruber R (2015) Bone-conditioned medium changes gene expression in bone-derived fibroblasts. Int J Oral Maxillofac Implants

28.

Schwartz Z, Mellonig JT, Carnes DL Jr, de la Fontaine J, Cochran DL, Dean DD, Boyan BD (1996) Ability of commercial demineralized freeze-dried bone allograft to induce new bone formation. J Periodontol 67:918–926. doi:10.1902/jop.1996.67.9.918

29.

Schwartz Z, Somers A, Mellonig JT, Carnes DL Jr, Dean DD, Cochran DL, Boyan BD (1998) Ability of commercial demineralized freeze-dried bone allograft to induce new bone formation is dependent on donor age but not gender. J Periodontol 69:470–478. doi:10.1902/jop.1998.69.4.470

Title: Pre-coating deproteinized bovine bone mineral (DBBM) with bone-conditioned medium (BCM) improves osteoblast migration, adhesion, and differentiation in vitro
Authors: Jordi Caballé-Serrano
Masako Fujioka-Kobayashi
Dieter D. Bosshardt
Reinhard Gruber
Daniel Buser
Richard J. Miron
Publication date: 01-12-2016
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 9/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-1747-x

At a glance: The STEP trials

Springer Medicine

Pre-coating deproteinized bovine bone mineral (DBBM) with bone-conditioned medium (BCM) improves osteoblast migration, adhesion, and differentiation in vitro

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Please log in to get access to this content

Other articles of this Issue 9/2016

Efficacy of fluoride varnish for preventing white spot lesions and gingivitis during orthodontic treatment with fixed appliances—a prospective randomized controlled trial

Leukocyte receptor expression in chronic periodontitis

Zinc-modified nanopolymers improve the quality of resin–dentin bonded interfaces

Was molar incisor hypomineralisation (MIH) present in archaeological case series?

Comparative release of growth factors from PRP, PRF, and advanced-PRF

Cold plasma: a novel approach to treat infected dentin—a combined ex vivo and in vitro study