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Open Access 29-11-2023

Practical Compass of Single-Cell RNA-Seq Analysis

Authors: Hiroyuki Okada, Ung-il Chung, Hironori Hojo

Published in: Current Osteoporosis Reports

Abstract

Purpose of Review

This review paper provides step-by-step instructions on the fundamental process, from handling fastq datasets to illustrating plots and drawing trajectories.

Recent Findings

The number of studies using single-cell RNA-seq (scRNA-seq) is increasing. scRNA-seq revealed the heterogeneity or diversity of the cellular populations. scRNA-seq also provides insight into the interactions between different cell types. User-friendly scRNA-seq packages for ligand-receptor interactions and trajectory analyses are available. In skeletal biology, osteoclast differentiation, fracture healing, ectopic ossification, human bone development, and the bone marrow niche have been examined using scRNA-seq. scRNA-seq data analysis tools are still being developed, even at the fundamental step of dataset integration. However, updating the latest information is difficult for many researchers. Investigators and reviewers must share their knowledge of in silico scRNA-seq for better biological interpretation.

Summary

This review article aims to provide a useful guide for complex analytical processes in single-cell RNA-seq data analysis.

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Title: Practical Compass of Single-Cell RNA-Seq Analysis
Authors: Hiroyuki Okada
Ung-il Chung
Hironori Hojo
Publication date: 29-11-2023
Publisher: Springer US
Published in: Current Osteoporosis Reports
Print ISSN: 1544-1873
Electronic ISSN: 1544-2241
DOI: https://doi.org/10.1007/s11914-023-00840-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Practical Compass of Single-Cell RNA-Seq Analysis

Abstract

Purpose of Review

Recent Findings

Summary

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose of Review

Recent Findings

Summary

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