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01-12-2023 | Original Article

Potential involvement of IL-32 in cell-to-cell communication between macrophages and hepatoblastoma

Authors: Ahmad Adawy, Lianbo Li, Hiroki Hirao, Tomoaki Irie, Daiki Yoshii, Hiromu Yano, Yukio Fujiwara, Shigeyuki Esumi, Masaki Honda, Shinya Suzu, Yoshihiro Komohara, Taizo Hibi

Published in: Pediatric Surgery International | Issue 1/2023

Abstract

Purpose

This study investigated the expression of interleukin 32 (IL-32) in hepatoblastoma, the most common primary pediatric liver tumor, and its possible roles in tumorigenesis.

Methods

IL-32 expression was investigated in two hepatoblastoma cell lines (Hep G2 and HuH 6) in the steady state and after co-culture with macrophages by RNA-seq analysis and RT-qPCR, and after stimulation with chemotherapy. Cultured macrophages were stimulated by IL-32 isoforms followed by RT-qPCR and western blot analysis. IL-32 immunohistochemical staining (IHC) was performed using specimens from 21 hepatoblastoma patients. Clustering analysis was also performed using scRNA-seq data downloaded from Gene Expression Omnibus.

Results

The IL-32 gene is expressed by hepatoblastoma cell lines; expression is upregulated by paracrine cell–cell communication with macrophages, also by carboplatin and etoposide. IL-32 causes protumor activation of macrophages with upregulation of PD-L1, IDO-1, IL-6, and IL-10. In the patient pool, IHC was positive only in 48% of cases. However, in the downloaded dataset, IL-32 gene expression was negative.

Conclusion

IL-32 was detected in hepatoblastoma cell lines, but not in all hepatoblastoma patients. We hypothesized that stimulation such as chemotherapy might induce expression of IL-32, which might be a critical mediator of chemoresistance in hepatoblastoma through inducing protumor activation in macrophages.

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Title: Potential involvement of IL-32 in cell-to-cell communication between macrophages and hepatoblastoma
Authors: Ahmad Adawy
Lianbo Li
Hiroki Hirao
Tomoaki Irie
Daiki Yoshii
Hiromu Yano
Yukio Fujiwara
Shigeyuki Esumi
Masaki Honda
Shinya Suzu
Yoshihiro Komohara
Taizo Hibi
Publication date: 01-12-2023
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 1/2023
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-023-05557-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Potential involvement of IL-32 in cell-to-cell communication between macrophages and hepatoblastoma

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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