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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2011

Open Access 01-12-2011 | Research article

Polysaccharides from Agaricus bisporus and Agaricus brasiliensis show similarities in their structures and their immunomodulatory effects on human monocytic THP-1 cells

Authors: Fhernanda R Smiderle, Andrea C Ruthes, Jeroen van Arkel, Wasaporn Chanput, Marcello Iacomini, Harry J Wichers, Leo JLD Van Griensven

Published in: BMC Complementary Medicine and Therapies | Issue 1/2011

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Abstract

Background

Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. Crude mushroom extracts have been tested without detailed chemical analyses of its polysaccharide content. For the present study we decided to chemically determine the carbohydrate composition of semi-purified extracts from 2 closely related and well known basidiomycete species, i.e. Agaricus bisporus and A. brasiliensis and to study their effects on the innate immune system, in particular on the in vitro induction of pro-inflammatory cytokines, using THP-1 cells.

Methods

Mushroom polysaccharide extracts were prepared by hot water extraction and precipitation with ethanol. Their composition was analyzed by GC-MS and NMR spectroscopy. PMA activated THP-1 cells were treated with the extracts under different conditions and the production of pro-inflammatory cytokines was evaluated by qPCR.

Results

Semi-purified polysaccharide extracts of A. bisporus and A. brasiliensis (= blazei) were found to contain (1→6),(1→4)-linked α-glucan, (1→6)-linked β-glucan, and mannogalactan. Their proportions were determined by integration of 1H-NMR signs, and were considerably different for the two species. A. brasiliensis showed a higher content of β-glucan, while A. bisporus presented mannogalactan as its main polysaccharide. The extracts induced a comparable increase of transcription of the pro-inflammatory cytokine genes IL-1β and TNF-α as well as of COX-2 in PMA differentiated THP-1 cells. Pro-inflammatory effects of bacterial LPS in this assay could be reduced significantly by the simultaneous addition of A. brasiliensis extract.

Conclusions

The polysaccharide preparations from the closely related species A. bisporus and A. brasiliensis show major differences in composition: A. bisporus shows high mannogalactan content whereas A. brasiliensis has mostly β-glucan. Semi-purified polysaccharide extracts from both Agaricus species stimulated the production of pro-inflammatory cytokines and enzymes, while the polysaccharide extract of A. brasiliensis reduced synthesis of these cytokines induced by LPS, suggesting programmable immunomodulation.
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Literature
1.
Moradali MF, Mostafavi H, Ghods S, Hedjaroude GA: Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi). Int Immunopharmacol. 2007, 7: 701-724. 10.1016/j.intimp.2007.01.008.CrossRefPubMed
2.
Zhang M, Cui SW, Cheung PCK, Wang Q: Antitumor polysaccharide from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends in Food Sci Technol. 2007, 18: 4-19. 10.1016/j.tifs.2006.07.013.CrossRef
3.
Ramberg JE, Nelson ED, Sinnott RA: Immunomodulatory dietary polysaccharides: a systematic review of the literature. Nutr J. 2010, 9 (54): 1-22.
4.
Schepetkin IA, Quinn MT: Botanical polysaccharides: Macrophage immunomodulation and therapeutic potential. Int Immunopharmacol. 2006, 6: 317-333. 10.1016/j.intimp.2005.10.005.CrossRefPubMed
5.
6.
Thompson IJ, Oyston PCF, Williamson DE: Potential of the β-glucans to enhance innate resistance to biological agents. Expert Rev Anti Infect Ther. 2010, 8 (3): 339-352. 10.1586/eri.10.10.CrossRefPubMed
7.
Lull C, Wichers HJ, Savelkoul HFJ: Antiinflammatory and immunomodulating properties of fungal metabolites. Med Inflammation. 2005, 2: 63-80.CrossRef
8.
Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K: Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 1980, 26: 171-176. 10.1002/ijc.2910260208.CrossRefPubMed
9.
Kohro T, Tanaka T, Murakami T, Wada Y, Aburatani H, Hamakubo T, Kodama T: A comparison of differences in the gene expression profiles of phorbol-12-myristate 13-acetate differentiated THP-1 cells and human monocytederived macrophage. J Atheroscl Thromb. 2004, 11: 88-97. 10.5551/jat.11.88.CrossRef
10.
Daigneault M, Preston JA, Marriott HM, Whyte MKB, Dockrell DH: The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. Plosone. 2010, 5 (1): 1-10.CrossRef
11.
Chan WK, Cheung CCH, Law HKW, Lau YL, Chan GCF: Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function. J Hematol Oncol. 2008, 1-9.
12.
Hsu JW, Huang HC, Chen ST, Wong CH, Juan HF: Ganoderma lucidum polysaccharides induce macrophage-like differentiation in human leukemia THP-1 cells via caspase and p53 activation. Evidence-Based Comp Alternat Med. 2011, 1-13.
13.
Duncan CJG, Pugh N, Pasco DS, Ross SA: Isolation of a galactomannan that enhances macrophage activation from the edible fungus Morchella esculenta. J Agric food chem. 2002, 50: 5683-5685. 10.1021/jf020267c.CrossRefPubMed
14.
Sakagami Y, Mizoguchi Y, Shin T, Seki S, Kobayashi K, Morisawa S, Yamamoto S: Effects of an anti-tumor polysaccharide, schizophyllan, on interferon-γ and interleukin 2 production by peripheral blood mononuclear cells. Biochem Biophys Res Comm. 1988, 155: 650-655. 10.1016/S0006-291X(88)80544-8.CrossRefPubMed
15.
Rubin-Bejerano I, Abeijon C, Magnelli P, Grisafi P, Fink GR: Phagocytosis by human neutrophils is stimulated by a unique fungal cell wall component. Cell Host Microb. 2007, 2: 55-67. 10.1016/j.chom.2007.06.002.CrossRef
16.
Mizuno T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T, Asakura A: Antitumor activity and some properties of water-soluble polysaccharides from "Himematsutake", from the fruiting body of Agaricus blazei Murill. Agric Biol Chem. 1990, 54 (11): 2889-2896. 10.1271/bbb1961.54.2889.
17.
Ito H, Shimura K, Itoh H, Kawade M: Antitumor effects of a new polysaccharide-protein complex (ATOM) prepared from Agaricus blazei (Iwade strain 101) "Himematsutake" and its mechanisms in tumor-bearing mice. Anticancer Res. 1997, 17 (1A): 277-84.PubMed
18.
Gonzaga MLC, Ricardo NMPS, Heatley F, Soares SA: Isolation and characterization of polysaccharides from Agaricus blazei Murill. Carbohydr Polym. 2005, 60: 43-49. 10.1016/j.carbpol.2004.11.022.CrossRef
19.
Chanput W, Mes J, Vreeburg RAM, Savelkoul HFJ, Wichers HJ: Transcription profiles of LPS-stimulated THP-1 monocytes and macrophages: a tool to study inflammation modulating effects of food-derived compounds. Food Funct. 2010, 1: 254-261. 10.1039/c0fo00113a.CrossRefPubMed
20.
Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔC T method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.CrossRefPubMed
21.
Wei S, Van Griensven LJLD: Pro- and antioxidative properties of medicinal mushroom extracts. Int J Med Mushr. 2008, 10 (4): 315-324. 10.1615/IntJMedMushr.v10.i4.30.CrossRef
22.
Sassaki GL, Souza LM, Serrato RV, Cipriani TR, Gorin PAJ, Iacomini M: Application of acetates derivatives for gas chromatography-mass spectrometry: Novel approaches on carbohydrates, lipids and amino acids analysis. J Chromatogr A. 2008, 1208: 215-222. 10.1016/j.chroma.2008.08.083.CrossRefPubMed
23.
Sassaki GL, Gorin PAJ, Souza LM, Czelusniak PA, Iacomini M: Rapid synthesis of partially O-methylated alditol acetate standards for GC-MS: some relative activities of hydroxyl groups of methyl glycopyranosides on Purdie methylation. Carbohydr Res. 2005, 340: 731-739. 10.1016/j.carres.2005.01.020.CrossRefPubMed
24.
Smiderle FR, Sassaki GL, van Arkel J, Iacomini M, Wichers HJ, van Griensven JLD: High molecular weight glucan of the culinary medicinal mushroom Agaricus bisporus is an α-glucan that forms complexes with low molecular weight galactan. Molecules. 2010, 15: 5818-5830. 10.3390/molecules15085818.CrossRefPubMed
25.
Ainsworth EA, Gillespie KM: Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protocols. 2007, 2: 4-875-877CrossRef
26.
Wei S, Helsper JPFG, Van Griensven LJLD: Phenolic compounds present in medicinal mushroom extracts generate reactive oxygen species in human cells in vitro. Int J Med Mushr. 2008, 10 (1): 1-13. 10.1615/IntJMedMushr.v10.i1.20.CrossRef
27.
Renard CMCG, Baron A, Guyot S, Drilleau JF: Interactions between apple cell walls and native apple polyphenols quantification and some consequences. Int J Biol Macromol. 2001, 29: 115-25. 10.1016/S0141-8130(01)00155-6.CrossRefPubMed
28.
Jakovljevic D, Miljkovic-Stojanovic J, Radulovic M, Hranisavljevic-Jakovljevic M: On the mannogalactan from the fruit bodies of Pleurotus ostreatus (Fr.) Quél. J Serb Chem Soc. 1998, 63 (2): 137-142.
29.
Smiderle FR, Olsen LM, Carbonero ER, Marcon R, Baggio C, Freitas C, Santos ARS, Torri G, Gorin PAJ, Iacomini M: A 3-O-methylated mannogalactan from Pleurotus pulmonarius: Structure and antinociceptive effect. Phytochem. 2008, 69 (15): 2731-2736. 10.1016/j.phytochem.2008.08.006.CrossRef
30.
Carbonero ER, Gracher AHP, Rosa MCC, Torri G, Sassaki GL, Gorin PAJ, Iacomini M: Unusual partially 3-O-methylated α-galactan from mushrooms of the genus Pleurotus. Phytochem. 2008, 69: 252-257. 10.1016/j.phytochem.2007.06.018.CrossRef
31.
Schlesinger LS, Hull SR, Kaufman TM: Binding of terminal mannosyl units of lipoarabinomannan from a virulent strain of Mycobacterium tuberculosis to human macrophages. J Immunol. 1994, 152 (8): 4070-4079.PubMed
32.
Mizuno T, Inagaki R, Kanao T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T, Asakura A: Antitumor activity and some properties of water-insoluble hetero-glycans from "Himematsutake", the fruiting body of Agaricus blazei Murill. Agric Biol Chem. 1990, 54 (11): 2897-2905. 10.1271/bbb1961.54.2897.
33.
Armstrong L, Jordan N, Millar A: Interleukin 1 0 (IL-1) regulation of tumour necrosis factor cx (TNF-cx) from human alveolar macrophages and peripheral blood monocytes. Thorax. 1996, 51: 143-149. 10.1136/thx.51.2.143.CrossRefPubMedPubMedCentral
34.
Sato TA, Keelan JA, Mitchell MD: Critical paracrine interactions between TNF-α and IL-10 regulate lipopolysaccharide-stimulated human chloriodecidual cytokine and prostaglandin E2 production. J Immunol. 2003, 170: 158-166.CrossRefPubMed
35.
Camelini CM, Maraschin M, Mendonc MM, Zucco C, Ferreira AG, Tavares LA: Structural characterization of β-glucans of Agaricus brasiliensis in different stages of fruiting body maturity and their use in nutraceutical products. Biotechnol Lett. 2005, 27: 1295-1299. 10.1007/s10529-005-0222-6.CrossRefPubMed
36.
Yamac M, Kanbak G, Zeytinoglu M, Senturk H, Bayramoglu G, Dokumacioglu A, Van Griensven LJLD: Pancreas protective effect of antioxidative Agaricus bisporus extract on rats with streptozotocin induced diabetes. Int J Med Mushr. 2010, 12 (4): 379-389. 10.1615/IntJMedMushr.v12.i4.50.CrossRef
37.
Jeong SC, Jeong YT, Yang BK, Islam R, Koyyalamudi SR, Pang G, Cho KY, Song CH: White button mushroom (Agaricus bisporus) lowers blood glucose and cholesterol levels in diabetic and hypercholesterolemic rats. Nutr Res. 2010, 30: 49-56. 10.1016/j.nutres.2009.12.003.CrossRefPubMed
38.
Volman JJ, Helsper JPFG, Wei S, Baars JJP, Van Griensven LJLD, Sonnenberg ASM, Mensink RP, Plat J: Effects of mushroom-derived b-glucan-rich polysaccharide extracts on nitric oxide production by bone marrow-derived macrophages and nuclear factor-jB transactivation in Caco-2 reporter cells: Can effects be explained by structure?. Mol Nutr Food Res. 2010, 54: 268-276. 10.1002/mnfr.200900009.CrossRefPubMed
39.
Tada R, Ikeda F, Aoki K, Yoshikawa M, Kato Y, Adachi Y, Tanioka A, Ishibashi K, Tsubaki K, Ohno N: Barley-derived β-D-glucan induces immunostimulation via a dectin-1-mediated pathway. Immunol Lett. 2009, 123: 144-148. 10.1016/j.imlet.2009.03.005.CrossRefPubMed
40.
Fujuhara M, Muroi M, Tanamoto K, Suzuki T, Azuma H, Ikeda H: Molecular mechanisms of macrophage activation and deactivation by lipopolysaccharide: roles of the receptor complex. Pharmacol Ther. 2003, 100: 171-194. 10.1016/j.pharmthera.2003.08.003.CrossRef
41.
Metadata
Title
Polysaccharides from Agaricus bisporus and Agaricus brasiliensis show similarities in their structures and their immunomodulatory effects on human monocytic THP-1 cells
Authors
Fhernanda R Smiderle
Andrea C Ruthes
Jeroen van Arkel
Wasaporn Chanput
Marcello Iacomini
Harry J Wichers
Leo JLD Van Griensven
Publication date
01-12-2011
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2011
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-11-58

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