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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2021

Open Access 01-12-2021 | Research

Polyphenol-rich extract of Ocimum gratissimum leaves prevented toxic effects of cyclophosphamide on the kidney function of Wistar rats

Authors: Quadri K. Alabi, Rufus O. Akomolafe, Joseph G. Omole, Ayodeji Aturamu, Mokolade S. Ige, Oyindasola O. Kayode, Deborah Kajewole-Alabi

Published in: BMC Complementary Medicine and Therapies | Issue 1/2021

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Abstract

Background

Cyclophosphamide (CP) is one of the potent and low cost chemotherapy used in clinical setting against a variety of tumors. However, its association with nephrotoxicity limits its therapeutic use. Ocimum gratissimum leaf is a medicinal plant with numerous pharmacological and therapeutic efficacies, such as antioxidant, anti-inflammation, and anti-apoptotic properties.

Methods

The present study was designed to evaluate the protective effect of Ocimum gratissimum (OG) against CP-induced kidney dysfunction in rats. Rats were pre-treated with 400 mg/kg b.w. of leave extract of Ocimum gratissimum (Ocimum G.) for 4 days and then 50 mg/kg b.w. of CP was co-administered from day 5 to day 7 along with Ocimum G. Markers of renal function and oxidative stress, food and water intake, electrolytes, aldosterone, leukocytes infiltration, inflammation and histopathological alteration were evaluated.

Results

Obvious renal inflammation and kidney injuries were observed in CP treated groups. However, administration of leave extract of Ocimum G. prevented oxidative stress, kidney injuries, attenuated inflammation, increased aldosterone production and reduced sodium ion and water loss in rats. The plasma creatinine, urea and urine albumin concentration were normalized after the administration of Ocimum G. extract in rats treated with CP. Ocimum G. also decreased the plasma concentrations of Interleukin-(IL)-6, C-reactive protein and activity of myeloperoxidase and malondialdehyde in CP treated rats.

Conclusion

Ocimum G. prevented kidney injury and enhanced renal function via inhibiting inflammation and oxidant-induced CP toxicity. The efficacy of Ocimum G. is related to the presence of various phytochemicals in the plant.
Literature
1.
Chabner BA, Ryand DP, Pax-Ares L, Garcis-Carbonero, Calaresi P. Antineoplastic agents. In: Harnam JG, Libmirde LE, editors. Goodman and Gilman’s the pharmacological basis of therapeutics. 10th ed. NewYork: McGraw Hill; 2001. p. 1389–459.
2.
Chabner BA, et al. Antineoplastic agents. In: Brunton LL, Lazo JS, Parker KL, editors. Goodman and Gilman’s the pharmacological basis of therapeutics. 11th ed. NewYork: McGraw Hill; 2006. p. 1322–8. 1694.
3.
Ge B, Yang D, Wu X, Zhu J, Wei W, Yang B. Cytoprotective effects of glycyrrhetinic acid liposome against cyclophosphamide-induced cystitis through inhibiting inflammatory stress. Int Immunopharmacol. 2018;54:139–44.PubMedCrossRef
4.
5.
Caglayan C, Temel Y, Kandemir FM, Yildirim S, Kucukler S. Naringin protects against cyclophosphamide-induced hepatotoxicity and nephrotoxicity through modulation of oxidative stress, inflammation, apoptosis, autophagy, and DNA damage. Environ Sci Pollut Res Int. 2018;25(21):20968–84.PubMedCrossRef
6.
Zhang J, Tian Q, Yung S, Chuen S, Zhou S, Duan W, et al. Metabolism and transport of oxazaphosphorines and the clinical implications. Drug Metab Rev. 2005;37:611–703.PubMedCrossRef
7.
Famurewa AC, Edeogu CO, Offor FI, Besong EE, Akunna GG, Maduagwuna EK. Downregulation of redox imbalance and iNOS/NF-ĸB/caspase-3 signalling with zinc supplementation prevents urotoxicity of cyclophosphamide-induced hemorrhagic cystitis in rats. Life Sci. 2021;266:118913.PubMedCrossRef
8.
Oyagbemi AA, Omobowale TO, Saba AB, Olowu ER, Dada RO, Akinrinde AS. Gallic acid ameliorates cyclophosphamide- induced neurotoxicity in wistar rats through free radical scavenging activity and improvement in antioxidant defense system. J Diet Suppl. 2016;13:402–19.PubMedCrossRef
9.
Abraham P, Isaac B. The effects of oral glutamine on cyclophosphamide-induced nephrotoxicity in rats. Hum Exp Toxicol. 2010;30(7):616–23.PubMedCrossRef
10.
Sherif IO. Uroprotective mechanism of quercetin against cyclophosphamideinduced urotoxicity: effect on oxidative stress and inflammatory markers. J Cell Biochem. 2018;119(9):7441–8.PubMedCrossRef
11.
12.
Effraim KD, Jacks TW, Sodipo OA. Histopathological studies on the toxicity of Ocimum gratissimum leave extract on some organs of rabbit. Afr J Biomed Res. 2003;6:21–5.
13.
Akinmoladun AC, Ibukun EO, Emmanuel A, Obuotor EM, Farombi EO. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci Res Essays. 2007;2:163–6.
14.
Alabi QK, Akomolafe RO, Omole JG, Adefisayo MA, Ogundipe OL, Aturamu A, et al. Polyphenol rich extract of Ocimum gratissimum leaves ameliorates colitis via attenuating colonic mucosa injury and regulating pro-inflammatory cytokines production and oxidative stress in rats. Biomed Pharmacother. 2018;103:812–22.PubMedCrossRef
15.
Offiah VN, Chikwendu UA. Antidiarrhoeal effects of Ocimum gratissimum leaf extract in experimental animals. J Ethnopharmacol. 1999;68:327–30.PubMedCrossRef
16.
Ezekwesili CN, Obiorah KA, Ugwu OP. Evaluation of anti -diarrheal effect of Ocimum gratissimum crude extract on albino rats. Biokemistri. 2004;16:122–31.
17.
Olamilosoye KP, Akomolafe RO, Akinsomisoye OS, Adefisayo MA, Alabi QK. The aqueous extract of Ocimum gratissimum leaves ameliorates acetic acid-induced colitis via improving antioxidant status and hematological parameters in male Wistar rats. Egypt J Basic Appl Sci. 2018;5:220–7.CrossRef
18.
Ijeh II, Omodamiro OD, Nwanna IJ. Antimicrobial effects of aqueous and ethanolic fractions of two spices, Ocimum gratissimum and Xylopia aethiopica. Afr J Biotechnol. 2005;4:953–6.
19.
Nweze EI, Eze EE. Justification for the use of Ocimum gratissimum L in herbal medicine and its interaction with disc antibiotics. BMC Complement Altern Med. 2009;9:32.CrossRef
20.
Shittu STT, Oyeyemi WA, Lasisi TJ, Shittu SA, Lawal TT, Olujobi ST. Aqueous leaf extract of Ocimum gratissimum improves hematological parameters in alloxan-induced diabetic rats via its antioxidant properties. Int J Appl Basic Med Res. 2016;6:96–100.PubMedPubMedCentralCrossRef
21.
Adesina TD, Nwinyi OC, Olugbuyiro JA. Prevention of bacterial biofilms formation on urinary catheter by selected plant extracts. Pak J Biol Sci. 2015;18(2):67–73.PubMedCrossRef
22.
Igbinosa EO, Idemudia OG. Anti-vibrio potentials of acetone and aqueous leaf extracts of Ocimum gratissumum (Linn). Trop J Pharm Res. 2016;15(4):743–50.CrossRef
23.
Keziah EA, Nukenine EN, Danga SP, Younoussa L, Esimone CO. Creams formulated with Ocimum gratissimum L. and Lantana camara L. crude extracts and fractions as mosquito repellents against Aedes aegypti L. (Diptera: Culicidae). J Insect Sci. 2015;15(1):45.PubMedPubMedCentralCrossRef
24.
Akinrinde AS, Oyagbemi AA, Omobowale TO, Asenuga ER, Ajibade TO. Alterations in blood pressure, antioxidant status and caspase 8 expression in cobalt chloride-induced cardio-renal dysfunction are reversed by Ocimum gratissimum and gallic acid in Wistar rats. J Trace Elem Med Biol. 2016;36:27–37.PubMedCrossRef
25.
Li PC, Chiu YW, Lin YM, et al. Herbal supplement ameliorates cardiac hypertrophy in rats with CCl (4)-induced liver cirrhosis. Evid Based Complement Alternat Med. 2012;2012:139045.PubMedPubMedCentralCrossRef
26.
Chiu YW, Chao PY, Tsai CC, et al. Ocimum gratissimum is effective in prevention against liver fibrosis in vivo and in vitro. Am J Chin Med. 2014;42(4):833–52.PubMedCrossRef
27.
Ofem OE, Eno AE, Antai AB. Gastric acid anti-secretory, anti-ulcerogenic and mucogenic effects of aqueous leaves’ extract of Ocimum gratissimum in rats. Niger J Physiol Sci. 2012;27(1):41–7.PubMed
28.
Tshilanda DD, Onyamboko DN, Babady-Bila P, et al. Anti-sickling activity of ursolic acid isolated from the leaves of Ocimum gratissimum L. (Lamiaceae). Nat Prod Bioprospect. 2015;5(4):215–21.PubMedPubMedCentralCrossRef
29.
Nangia-Makker P, Raz T, Tait L, et al. Ocimum gratissimum retards breast cancer growth and progression and is a natural inhibitor of matrix metalloproteases. Cancer Biol Ther. 2013;14(5):417–27.PubMedPubMedCentralCrossRef
30.
Pandey H, Pandey P, Singh S, Gupta R, Banerjee S. Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation. Protoplasma. 2015;252(2):647–55.PubMedCrossRef
31.
Pereira SL, de Oliveira JW, Angelo KK, da Costa AM, Costa F. Clinical effect of a mouth rinse containing Ocimum gratissimum on plaque and gingivitis control. J Contemp Dent Pract. 2011;12(5):350–5.PubMedCrossRef
32.
Singh P, Jayaramaiah RH, Agawane SB, et al. Potential dual role of eugenol in inhibiting advanced glycation end products in diabetes: proteomic and mechanistic insights. Sci Rep. 2016;6:18798.PubMedPubMedCentralCrossRef
33.
Pires AF, Madeira SV, Soares PM, et al. The role of endothelium in the vasorelaxant effects of the essential oil of Ocimum gratissimum in aorta and mesenteric vascular bed of rats. Can J Physiol Pharmacol. 2012;90(10):1380–5.PubMedCrossRef
34.
Kavaz D, Idris M, Onyebuchi C. Physiochemical characterization, antioxidative, anticancer cells proliferation and food pathogens antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus rhizome essential oils. Int J Biol Macromol. 2019;123:837–45.PubMedCrossRef
35.
Gulcin I, Oktay M, Kirecci E, Kufrevioglu OI. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem. 2003;83:371–82.CrossRef
36.
Rakmai J, Cheirsilp B, Mejuto J, Torrado-Agrasar A, Simal-Gándara J. Physico-chemical characterization and evaluation of bio-efcacies of black pepper essential oil encapsulated in hydroxypropyl-beta-cyclodextrin. Food Hydrocoll. 2017;65:157–64.CrossRef
37.
Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. Improving bioscience research reporting the ARRIVE guidelines for reporting animal research. PLoS Biol. 2010;8(6):e1000412.PubMedPubMedCentralCrossRef
38.
Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972;247:3170–5.PubMedCrossRef
39.
40.
Beutler E, et al. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963;51:882–8.
41.
Wolff SP. Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods Enzymol. 1994;233:182–9.CrossRef
42.
Ohkawa H, et al. Assay for lipid peroxide in animal tissues by thiobarbutiric acid reaction. Anal Biochem. 1979;95:351–8.PubMedCrossRef
43.
Xia Y, Zweier JL. Measurement of myeloperoxidase in leukocyte-containing tissues. Anal Biochem. 1997;245:93–6.PubMedCrossRef
44.
Alabi QK, Akomolafe RO, Olukiran OS, Nafiu AO, Adefisayo MA, Owotomo OI, et al. Combined administration of l-carnitine and ascorbic acid ameliorates cisplatin-induced nephrotoxicity in rats. J Am Coll Nutr. 2018;37(5):387–98.PubMedCrossRef
45.
Kern JC, Kehrer JP. Acrolein-induced cell death: a caspaseinfluenced decision between apoptosis and oncosis/necrosis. Chem Biol Interact. 2002;139:79–95.PubMedCrossRef
46.
47.
Nafees S, Rashid S, Ali N, Hasan SK, Sultana S. Rutin ameliorates cyclophosphamide induced oxidative stress and inflammation in Wistar rats: role of NFκB/MAPK pathway. Chem Biol Interact. 2015;231:98–107.PubMedCrossRef
48.
Bray C, Bell LN, Liang H, Haykal R, Kaiksow F, Mazza JJ, et al. Erythrocyte sedimentation rate and C-reactive protein measurements and their relevance in clinical medicine. WMJ. 2016;115(6):317–21.PubMed
49.
50.
Mantovani A, Muzio M, Garlanda C, Sozzani S, Allavena P. Macrophage control of inflammation: negative pathways of regulation of inflammatory cytokines. Novartis Found Symp. 2001;234:120–31.PubMed
51.
Omole JG, Alabi QK, Aturamu A, Adefisayo MA, Oluwayomi O, Dada MB, et al. Barium chloride dose-dependently induced heart and lung injury in Wistar rats. Environ Toxicol. 2019;34(12):1303–12.PubMedCrossRef
52.
Alabi QK, et al. Kolaviron attenuates diclofenac-induced nephrotoxicity in male Wistar rats. Appl Physiol Nutr Metab. 2018;43:956–68.PubMedCrossRef
53.
Song S, Meyer M, Turk TR, Wilde B, Feldkamp T, Assert R, et al. Serum cystatin C in mouse models: a reliable and precise marker for renal function and superior to serum creatinine. Nephrol Dial Transplant. 2009;24:1157–61.PubMedCrossRef
54.
Ogundipe DJ, Akomolafe RO, Sanusi AA, Imafidon CE, Olukiran OS, Oladele AA. Ocimum gratissimum ameliorates gentamicin-induced kidney injury but decreases creatinine clearance following sub-chronic administration in rats. J Evid Based Complementary Altern Med. 2017;22(4):592–602.PubMedPubMedCentralCrossRef
55.
56.
Stehouwer CD, Smulders YM. Microalbuminuria and risk for cardiovascular disease: analysis of potential mechanisms. J Am Soc Nephrol. 2006;17(8):2106–11.PubMedCrossRef
57.
Ranchoux B, Günther S, Quarck R, et al. Chemotherapy induced pulmonary hypertension: role of alkylating agents. Am J Pathol. 2015;185(2):356–71.PubMedCrossRef
58.
Mikaelian I, Buness A, de Vera-Mudry MC, et al. Primary endothelial damage is the mechanism of cardiotoxicity of tubulin-binding drugs. Toxicol Sci. 2010;117(1):144–51.PubMedCrossRef
59.
Bressler RB, Huston DP. Water intoxication following moderate-dose intravenous cyclophosphamide. Arch Intern Med. 1985;145(3):548–9.PubMedCrossRef
60.
Salido M, Macarron P, Hernandez-Garcia C, D’Cruz DP, Khamashta MA, Hughes GR. Water intoxication induced by low-dose cyclophosphamide in two patients with systemic lupus erythematosus. Lupus. 2003;12(8):636–9.PubMedCrossRef
61.
Nafiu AO, Akomolafe RO, Alabi QK, Idowu CO, Odujoko OO. Effect of fatty acids from ethanol extract of Moringa oleifera seeds on kidney function impairment and oxidative stress induced by gentamicin in rats. Biomed Pharmacother. 2019;117:109154.PubMedCrossRef
62.
Singh G, Fries JF, Williams CA, Zatarain E, Spitz P, Bloch DA. Toxicity profiles of disease modifying antirheumatic drugs in rheumatoid arthritis. J Rheumatol. 1996;18(2):188–94.
63.
Ogundipe DJ, Akomolafe RO, Sanusi AA, Imafidon CE, Olukiran OS, Oladele AA. Effects of two weeks administration of Ocimum gratissimum leaf on feeding pattern and markers of renal function in rats treated with gentamicin. Egypt J Basic Appl Sci. 2016;3(3):219–31.CrossRef
64.
National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th edition. Washington (DC): National Academies Press (US); 2011.
Metadata
Title
Polyphenol-rich extract of Ocimum gratissimum leaves prevented toxic effects of cyclophosphamide on the kidney function of Wistar rats
Authors
Quadri K. Alabi
Rufus O. Akomolafe
Joseph G. Omole
Ayodeji Aturamu
Mokolade S. Ige
Oyindasola O. Kayode
Deborah Kajewole-Alabi
Publication date
01-12-2021
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2021
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-021-03447-3

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