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Open Access 01-12-2011 | Research

Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China

Authors: Xiaofang Hao, Zengjun Lu, Wendong Kuang, Pu Sun, Yu Fu, Lei Wu, Qing Zhao, Huifang Bao, Yuanfang Fu, Yimei Cao, Pinghua Li, Xingwen Bai, Dong Li, Zaixin Liu

Published in: Virology Journal | Issue 1/2011

Abstract

Background

Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits extensive genetic variation. The outbreak of a highly pathogenic PRRS in 2006 led us to investigate the extent of PRRSV genetic diversity in China. To this end, we analyzed the Nsp2 and ORF7 gene sequences of 98 Chinese PRRSV isolates.

Results

Preliminary analysis indicated that highly pathogenic PRRSV strains with a 30-amino acid deletion in the Nsp2 protein are the dominant viruses circulating in China. Further analysis based on ORF7 sequences revealed that all Chinese isolates were divided into 5 subgroups, and that the highly pathogenic PRRSVs were distantly related to the MLV or CH-1R vaccine, raising doubts about the efficacy of these vaccines. The ORF7 sequence data also showed no apparent associations between geographic or temporal origin and heterogeneity of PRRSV in China.

Conclusion

These findings enhance our knowledge of the genetic characteristics of Chinese PRRSV isolates, and may facilitate the development of effective strategies for monitoring and controlling PRRSV in China.

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Title: Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China
Authors: Xiaofang Hao
Zengjun Lu
Wendong Kuang
Pu Sun
Yu Fu
Lei Wu
Qing Zhao
Huifang Bao
Yuanfang Fu
Yimei Cao
Pinghua Li
Xingwen Bai
Dong Li
Zaixin Liu
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-8-73

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