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01-11-2021 | Polymerase Chain Reaction | Original Article

Development and validation of novel 8-dye short tandem repeat multiplex system for forensic applications

Authors: Bowei Jiang, Weifeng Qu, Feng Wang, Liming Zhang, Haibo Rong, Jienan Li, Dan Wen, Moutanou Modeste Judes Zeye, Wei He, Chudong Wang, Hao Xing, Tao Zhang, Chuan Jin, Li Chen, Ying Liu, Jifeng Cai, Lagabaiyila Zha

Published in: International Journal of Legal Medicine | Issue 6/2021

Abstract

DNA profiling of short tandem repeats (STRs) is the primary method for genotyping forensic samples. However, degraded DNA and trace samples are still major problems for commercial 5- or 6-dye STR kits. In order to improve the performance of this method, we developed a novel 8-dye STR multiplex system containing 18 autosomal loci (D3S1358, D1S1656, TPOX, D16S539, vWA, D6S1043, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, TH01, D21S11, D12S391, and PentaD) and the sex-determining locus Amelogenin, with all fragments smaller than 330 bases. Validation was carried out as recommended by the Scientific Working Group on DNA Analysis Methods. The results showed that complete profiles were obtainable when the input DNA was as low as 0.0625 ng. Full profiles were obtained even in the presence of inhibitors such as humic acid (< 300 ng/μl), hematin (< 100 μM), and indigo (0.01%). The 8-dye STR multiplex system also showed good performance in the detection degraded DNA samples. These results indicate that the 8-dye STR multiplex system is suitable for human DNA genotyping, including for difficult forensic materials.

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Title: Development and validation of novel 8-dye short tandem repeat multiplex system for forensic applications
Authors: Bowei Jiang
Weifeng Qu
Feng Wang
Liming Zhang
Haibo Rong
Jienan Li
Dan Wen
Moutanou Modeste Judes Zeye
Wei He
Chudong Wang
Hao Xing
Tao Zhang
Chuan Jin
Li Chen
Ying Liu
Jifeng Cai
Lagabaiyila Zha
Publication date: 01-11-2021
Publisher: Springer Berlin Heidelberg
Keyword: Polymerase Chain Reaction
Published in: International Journal of Legal Medicine / Issue 6/2021
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-021-02695-9

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Development and validation of novel 8-dye short tandem repeat multiplex system for forensic applications

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