Top

European Archives of Paediatric Dentistry

Published in:

25-06-2022 | Polymerase Chain Reaction | Original Scientific Article

Comparative real-time PCR quantification of cytomegalovirus in severe early childhood caries and caries-free children

Authors: N. Nourbakhsh, L. Sadri, S. Moghim, S. Mohammadi

Published in: European Archives of Paediatric Dentistry | Issue 5/2022

Abstract

Purpose

The present study was undertaken to evaluate cytomegalovirus (CMV) load in carious lesions in children with severe early childhood caries (S-ECC) and compare it with caries-free (CF), healthy children, by quantitative real-time polymerase chain reaction (PCR).

Materials and methods

Dental plaque samples were collected from 57 subjects including S-ECC (n = 33) and CF (n = 24) patients. The two groups were matched in terms of age, gender, and socioeconomic status. After deoxyribonucleic acid (DNA) extraction, SYBR Green real-time PCR was used to quantify CMV DNA.

Results

The CMV DNA was detected in seven patients in the S-ECC group. The mean of decayed, missing, and filled tooth surfaces (dmfs) was 11.90 ± 5.95 in the S-ECC group. There was no significant difference in gender between the two groups. There was a significant relationship between CMV positivity in children and the S-ECC group (p = 0.043).

Conclusion

Results of this study suggest a relationship between the presence of CMV in the oral cavity and S-ECC. We can design clinical trials to confirm these results and improve children's dental care.

Affairs AA of PDC on C. Policy on early childhood caries (ECC): unique challenges and treatment options. Pediatr. Dent. 2005;27(7):34–5.

Amini kafiabad S, Ranjbar kermani F, Ferdousian F, Sobhani M, Samiei SH. Assessing the frequency of cytomegalovirus Viremia in Iranian (Tehran) blood donors by polymerase chain reaction method. Hakim Res J. 2009;12(3):61–67.

Arora N, Novak Z, Fowler KB, Boppana SB, Ross SA. Cytomegalovirus viruria and DNAemia in healthy seropositive women. J Infect Dis. 2010;202(12):1800–3. https://doi.org/10.1086/657412.CrossRefPubMedPubMedCentral

Clarke M, Locker D, Berall G, Pencharz P, Kenny DJ, Judd P. Malnourishment in a population of young children with severe early childhood caries. Pediatr Dent. 2006;28(3):254–9.PubMed

Cleaton-Jones P, Richardson BD, Granath L, Fatti LP, Sinwell R, Walker AR, et al. Nutritional status and dental caries in a large sample of 4-and 5-year-old South African children. South African Med J. 2000;90(6):631–5.

Collier AC, Meyers JD, Corey L, Murphy VL, Roberts PL, Handsfield HH. Cytomegalovirus infection in homosexual men: relationship to sexual practices, antibody to human immunodeficiency virus, and cell-mediated immunity. Am J Med. 1987;82(3):593–601. https://doi.org/10.1016/0002-9343(87)90105-7.CrossRefPubMed

Cunha BA. Cytomegalovirus pneumonia: community-acquired pneumonia in immunocompetent hosts. Infect Dis Clin North Am. 2010;24(1):147. https://doi.org/10.1016/j.idc.2009.10.008.CrossRefPubMedPubMedCentral

Dworkin LL, Gibler TM, Van Gelder RN. Real-time quantitative polymerase chain reaction diagnosis of infectious posterior uveitis. Arch Ophthalmol. 2002;120(11):1534–9. https://doi.org/10.1001/archopht.120.11.1534.CrossRefPubMed

Feldens CA, Giugliani ERJ, Vigo A, Vítolo MR. Early feeding practices and severe early childhood caries in four-year-old children from southern Brazil: a birth cohort study. Caries Res. 2010;44(5):445–52. https://doi.org/10.1159/000319898.CrossRefPubMed

Gonzalez CD, Okunseri C. Senior dental students’ experience with Cariogram in a pediatric dentistry clinic. J Dent Educ. 2010;74(2):123–9. https://doi.org/10.1002/j.0022-0337.2010.74.2.tb04861.CrossRefPubMed

Guinan M, Thomas P, Pinsky P, Goodrich J, Selik R, Jaffe H, et al. Heterosexual and homosexual patients with the acquired immunodeficiency syndrome: a comparison of surveillance, interview, and laboratory data. Ann Intern Med. 1984;100(2):213–8. https://doi.org/10.7326/0003-4819-100-2-213.CrossRefPubMed

Hafez HS, Shaarawy SM, Al-Sakiti AA, Mostafa YA. Dental crowding as a caries risk factor: a systematic review. Am J Orthod Dentofac Orthop. 2012;142(4):443–50. https://doi.org/10.1016/j.ajodo.2012.04.018.CrossRef

Iida H, Auinger P, Billings RJ, Weitzman M. Association between infant breastfeeding and early childhood caries in the United States. Pediatrics. 2007;120(4):e944–52. https://doi.org/10.1542/peds.2006-0124.CrossRefPubMed

Ismail AI, Sohn W. A systematic review of clinical diagnostic criteria of early childhood caries. J Public Health Dent. 1999;59(3):171–91. https://doi.org/10.1111/j.1752-7325.1999.tb03267.x.CrossRefPubMed

Li Y, Wang W. Predicting caries in permanent teeth from caries in primary teeth: an eight-year cohort study. J Dent Res. 2002;81(8):561–6. https://doi.org/10.1177/154405910208100812.CrossRefPubMed

Milgrom P, Riedy CA, Weinstein P, Tanner ACR, Manibusan L, Bruss J. Dental caries and its relationship to bacterial infection, hypoplasia, diet, and oral hygiene in 6-to 36-month-old children. Commun Dent Oral Epidemiol. 2000;28(4):295–306. https://doi.org/10.1034/j.1600-0528.2000.280408.x.CrossRef

Mohebbi SZ, Virtanen JI, Vahid-Golpayegani M, Vehkalahti MM. Early childhood caries and dental plaque among 1–3-year-olds in Tehran. Iran J Indian Soc Pedod Prev Dent. 2006;24(4):177.CrossRefPubMed

Okada M, Hayashi F, Nagasaka N. Detection of actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in dental plaque samples from children 2 to 12 years of age. J Clin Periodontol. 2000;27(10):763–8. https://doi.org/10.1034/j.1600-051x.2000.027010763.x.CrossRefPubMed

Proctor BD, Dalaker J. Poverty in the United States: 2002. US Department of Commerce, Census Bureau; 2003.

Saygun I, Kubar A, Özdemir A, Slots J. Periodontitis lesions are a source of salivary cytomegalovirus and Epstein-Barr virus. J Periodontal Res. 2005;40(2):187–91. https://doi.org/10.1111/j.1600-0765.2005.00790.x.CrossRefPubMed

Sharma S, Tapashetti RP, Patil SR, Kalra SM, Bhat GK, Guvva S. Revelation of viral–bacterial interrelationship in aggressive periodontitis via polymerase chain reaction: a microbiological study. J Int oral Heal. 2015;7(9):101.

Slade GD. Epidemiology of dental pain and dental caries among children and adolescents. Commun Dent Health. 2001;18(4):219–27.

Slots J, Saygun I, Sabeti M, Kubar A. Epstein-Barr virus in oral diseases. J Periodontal Res. 2006;41(4):235–44. https://doi.org/10.1111/j.1600-0765.2006.00865.x.CrossRefPubMed

Smithers G, Gregory JR, Bates CJ, Prentice A, Jackson LV, Wenlock R. The national diet and nutrition survey: young people aged 4–18 years. Nutr Bull. 2000;25(2):105–11. https://doi.org/10.1046/j.1467-3010.2000.00027.x.CrossRef

Tinanoff N, Kanellis MJ, Vargas CM. Current understanding of the epidemiology, mechanisms, and prevention of dental caries in preschool children. Pediatr Dent. 2002;24(6):543–51.PubMed

Van Houte J, Gibbs G, Butera C. Oral flora of children with nursing bottle caries. J Dent Res. 1982;61(2):382–5. https://doi.org/10.1177/00220345820610020201.CrossRefPubMed

Vargas CM, Macek MD, Goodman HS, Wagner ML. Dental pain in Maryland school children. J Public Health Dent. 2005;65(1):3–6. https://doi.org/10.1111/j.1752-7325.2005.tb02780.x.CrossRefPubMed

Weerheijm KL. Molar incisor hypomineralisation (MIH). Eur J Paediatr. 2003;4:115–20.

WHO. World Health Organization Oral Health Surveys-Basic Methods 5th edition. 2013;125.

Yildirim S, Yildiz E, Kubar A. TaqMan real-time quantification of Epstein-Barr virus in severe early childhood caries. Eur J Dent. 2010;4(1):28–33.CrossRefPubMedPubMedCentral

Zhang LJ, Hanff P, Rutherford C, Churchill WH, Crumpacker CS. Detection of human cytomegalovirus DNA, RNA, and antibody in normal donor blood. J Infect Dis. 1995;171(4):1002–6. https://doi.org/10.1093/infdis/171.4.1002.CrossRefPubMed

Title: Comparative real-time PCR quantification of cytomegalovirus in severe early childhood caries and caries-free children
Authors: N. Nourbakhsh
L. Sadri
S. Moghim
S. Mohammadi
Publication date: 25-06-2022
Publisher: Springer Berlin Heidelberg
Keywords: Polymerase Chain Reaction
Caries
Published in: European Archives of Paediatric Dentistry / Issue 5/2022
Print ISSN: 1818-6300
Electronic ISSN: 1996-9805
DOI: https://doi.org/10.1007/s40368-022-00711-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Comparative real-time PCR quantification of cytomegalovirus in severe early childhood caries and caries-free children

Abstract

Purpose

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Materials and methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 5/2022

Evaluation and comparison of cytotoxicity and bioactivity of chemomechanical caries removal agents on stem cells from human exfoliated deciduous teeth

Free sugars and early childhood caries development: a prospective cohort study

Clinical effectiveness of restorative materials for the restoration of carious primary teeth without pulp therapy: a systematic review

Evaluation of the efficacy of a 2-step etch-and-rinse, 2-step self-etch and 1-step self-etch adhesive systems, in class II primary molars restorations. An one year prospective, randomized clinical trial

Contemporary management of deep caries in primary teeth: a systematic review and meta-analysis

Minimal Intervention Dentistry (MID) mainstream or unconventional option? Study exploring the impact of COVID-19 on paediatric dentists’ views and practices of MID for managing carious primary teeth in children across the United Kingdom and European Union