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Open Access 01-12-2024 | Polycystic Ovary Syndrome | Research

Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H₂O₂-induced granulosa cells apoptosis via USP48 protein regulation

Authors: Jun Chen, Chuhua Lin, Xiurong Huang, Wei Bian

Published in: BMC Complementary Medicine and Therapies | Issue 1/2024

Abstract

Background

Oxidative stress and inflammation can lead to apoptosis of ovarian granulosa cells (GCs), resulting in ovulation disorders and infertility. Baicalin (BAI) promotes cell proliferation and reduces inflammation and oxidative stress. However, the mechanisms by which BAI treatment affects oxidative stress and inflammation in GCs remain incompletely understood.

Methods

KGN cells were treated with hydrogen peroxide (H₂O₂) to analyze the effect of oxidative stress on GCs in vitro. Subsequently, H₂O₂-stimulated KGN cells were treated with BAI. The levels of GSH-Px, CAT, and SOD were measured using an activity assay kit. The levels of MDA, IL-1β, IL-6, IL-8, and TNF-α were measured by ELISA. Proliferation, apoptosis, and mRNA and protein levels were measured using the CCK8, flow cytometry, qRT-PCR, and western blotting.

Results

H₂O₂ treatment inhibited KGN cell proliferation and promoted apoptosis, accompanied by increased oxidative stress and inflammation. BAI promoted proliferation, inhibited apoptosis, and reduced oxidative stress and inflammation in H₂O₂-stimulated KGN cells. BAI treatment promoted USP48 protein expression, and USP48 knockdown abrogated the protective effects of BAI, indicating that USP48 is a downstream mediator of BAI.

Conclusion

BAI treatment enhanced cell proliferation and ameliorated oxidative stress and inflammation by enhancing USP48 protein expression. BAI, which is used clinically and as a dietary supplement, may alleviate oxidative stress-induced GC injury and ovarian disorders.

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Title: Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H2O2-induced granulosa cells apoptosis via USP48 protein regulation
Authors: Jun Chen
Chuhua Lin
Xiurong Huang
Wei Bian
Publication date: 01-12-2024
Publisher: BioMed Central
Keywords: Polycystic Ovary Syndrome
Polycystic Ovary Syndrome
Published in: BMC Complementary Medicine and Therapies / Issue 1/2024
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-024-04346-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H₂O₂-induced granulosa cells apoptosis via USP48 protein regulation

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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