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01-06-2017 | Original Article

Podoplanin, ezrin, and Rho-A proteins may have joint participation in tumor invasion of lip cancer

Authors: Agnes Assao, Suely Nonogaki, José Roberto Pereira Lauris, André Lopes Carvalho, Clóvis Antônio Lopes Pinto, Fernando Augusto Soares, Luiz Paulo Kowalski, Denise Tostes Oliveira

Published in: Clinical Oral Investigations | Issue 5/2017

Abstract

Introduction

Podoplanin and ezrin connection through Rho-A phosphorylation have been suggested as part of the activation pathway, in the process of tumor invasion and cell movement in oral squamous cell carcinomas.

Objective

The aim of this study was to evaluate the correlation among podoplanin, ezrin, and Rho-A immunoexpressions in 91 squamous cells carcinomas of the lower lip and their influence in patient’s prognosis.

Material and methods

The immunoexpressions of podoplanin, ezrin, and Rho-A were evaluated through a semi-quantitative score method, based on the capture of 10 microscopic fields at the front of tumor invasion. The association and correlation of these proteins with the clinicopathological features were verified by Fischer’s exact test and Spearman’s test. The prognostic values were analyzed by Kaplan-Meier method and log-rank test.

Results

A statistically significant association between strong cytoplasmic podoplanin expression and alcohol (p = 0.024), loco-regional recurrences (p = 0.028), and lymph node metastasis (pN+) (p = 0.010) was found. The membranous (p = 0.000 and r = 0.384) and cytoplasmic (p = 0.000 and r = 0.344) podoplanin expression was statistically correlated with ezrin expression. Also, membranous podoplanin was significantly correlated with Rho-A expression (p = 0.006 and r = 0.282). The expressions of podoplanin, ezrin, and Rho-A were not significant prognostic factors for patients with squamous cell carcinomas of the lower lip.

Conclusions

Therefore, our results confirm a correlation among podoplanin, ezrin, and Rho-A expressions in squamous cell carcinoma of the lip suggesting a cooperative participation of these proteins in cell movement and invasion.

Clinical relevance

Furthermore, strong cytoplasmic podoplanin expression could be helpful to identify patients with squamous cell carcinoma of the lip and lower risk of loco-regional recurrences.

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Title: Podoplanin, ezrin, and Rho-A proteins may have joint participation in tumor invasion of lip cancer
Authors: Agnes Assao
Suely Nonogaki
José Roberto Pereira Lauris
André Lopes Carvalho
Clóvis Antônio Lopes Pinto
Fernando Augusto Soares
Luiz Paulo Kowalski
Denise Tostes Oliveira
Publication date: 01-06-2017
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 5/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-1956-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Podoplanin, ezrin, and Rho-A proteins may have joint participation in tumor invasion of lip cancer

Abstract

Introduction

Objective

Material and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Objective

Material and methods

Results

Conclusions

Clinical relevance

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