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Reproductive Biology and Endocrinology

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Open Access 01-12-2009 | Research

Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium

Authors: Michal Amir, Shabtai Romano, Shlomit Goldman, Eliezer Shalev

Published in: Reproductive Biology and Endocrinology | Issue 1/2009

Abstract

Background

To study the expression of Plexin-B1, Glycodelin, and MMP7 during the menstrual cycle in the endometrium and in the fallopian tube.

Methods

The research included women undergoing hysterectomy, tubal sterilization or salpingo-oophoerectomy. Total RNA from endometrial and fallopian tube tissues was extracted using a total RNA isolation kit. Semi-quantitative RT-PCR was performed to examine mRNA relative expression.

Results

Plexin-B1 expression in the endometrium was significantly higher on days 19 - 23 compared to days 12 - 14 (1.166 +/- 0.42 versus 0.523 +/- 0.299), P < 0.005. In the fallopian tube the level of plexin-B1 did not change significantly throughout the menstrual cycle. Glycodelin expression was significantly higher on days 19 - 23 compared with days 12-14, both in the endometrium (0.819 +/- 0.564 versus 0.072 +/- 0.343, P < 0.05) and the fallopian tube (0.796 +/- 0.196 versus 0.329 +/- 0.398, P < 0.05). Although the level of MMP7 secretion was the highest in the secretory phase the difference from the proliferative phase did not reach statistical significance, neither in the endometrium nor in the fallopian tube. This could result from a lack of power.

Conclusions

In the endometrium, both Glycodelin and Plexin-B1 are exhibiting a cyclic pattern suggesting a possible steroid regulation and a role in endometrial receptivity.

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Title: Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium
Authors: Michal Amir
Shabtai Romano
Shlomit Goldman
Eliezer Shalev
Publication date: 01-12-2009
Publisher: BioMed Central
Published in: Reproductive Biology and Endocrinology / Issue 1/2009
Electronic ISSN: 1477-7827
DOI: https://doi.org/10.1186/1477-7827-7-152

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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