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International Journal of Implant Dentistry

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Open Access 01-12-2017 | Research

Platelet-rich fibrin prepared from stored whole-blood samples

Authors: Kazushige Isobe, Masashi Suzuki, Taisuke Watanabe, Yutaka Kitamura, Taiji Suzuki, Hideo Kawabata, Masayuki Nakamura, Toshimitsu Okudera, Hajime Okudera, Kohya Uematsu, Koh Nakata, Takaaki Tanaka, Tomoyuki Kawase

Published in: International Journal of Implant Dentistry | Issue 1/2017

Abstract

Background

In regenerative therapy, self-clotted platelet concentrates, such as platelet-rich fibrin (PRF), are generally prepared on-site and are immediately used for treatment. If blood samples or prepared clots can be preserved for several days, their clinical applicability will expand. Here, we prepared PRF from stored whole-blood samples and examined their characteristics.

Methods

Blood samples were collected from non-smoking, healthy male donors (aged 27–67 years, N = 6), and PRF clots were prepared immediately or after storage for 1–2 days. Fibrin fiber was examined by scanning electron microscopy. Bioactivity was evaluated by means of a bioassay system involving human periosteal cells, whereas PDGF-BB concentrations were determined by an enzyme-linked immunosorbent assay.

Results

Addition of optimal amounts of a 10% CaCl₂ solution restored the coagulative ability of whole-blood samples that contained an anticoagulant (acid citrate dextrose) and were stored for up to 2 days at ambient temperature. In PRF clots prepared from the stored whole-blood samples, the thickness and cross-links of fibrin fibers were almost identical to those of freshly prepared PRF clots. PDGF-BB concentrations in the PRF extract were significantly lower in stored whole-blood samples than in fresh samples; however, both extracts had similar stimulatory effects on periosteal-cell proliferation.

Conclusions

Quality of PRF clots prepared from stored whole-blood samples is not reduced significantly and can be ensured for use in regenerative therapy. Therefore, the proposed method enables a more flexible treatment schedule and choice of a more suitable platelet concentrate immediately before treatment, not after blood collection.

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Title: Platelet-rich fibrin prepared from stored whole-blood samples
Authors: Kazushige Isobe
Masashi Suzuki
Taisuke Watanabe
Yutaka Kitamura
Taiji Suzuki
Hideo Kawabata
Masayuki Nakamura
Toshimitsu Okudera
Hajime Okudera
Kohya Uematsu
Koh Nakata
Takaaki Tanaka
Tomoyuki Kawase
Publication date: 01-12-2017
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Implant Dentistry / Issue 1/2017
Electronic ISSN: 2198-4034
DOI: https://doi.org/10.1186/s40729-017-0068-4

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Platelet-rich fibrin prepared from stored whole-blood samples

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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