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Archives of Virology
Published in: Archives of Virology 1/2013

01-01-2013 | Original Article

Phenotypic and molecular characteristics of plaque-purified MX10 virus, an Oriental-Australian genotype of Sindbis virus from Yunnan, China

Authors: Wang Jinglin, Zhu Wuyang, Shihong Fu, Li Wenjuan, Zhang Hailin, Guodong Liang

Published in: Archives of Virology | Issue 1/2013

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Abstract

A previous investigation showed that MX10 virus, recently isolated in China, belongs to the Oriental-Australian (O/A) genotype of Sindbis virus (SINV) (Wang Jinglin, 2011, ATMH). Similar to the MRE16 isolate, the prototype O/A genotype of SINV, two derivate viruses with obviously different plaque morphologies were derived from MX10 virus, which were accordingly denoted as MX10-LP and MX10-SP. MX10-LP virus exhibited higher neurovirulence in neonatal mice than MX10-SP virus. Analysis of the complete genome revealed seven nucleotide differences between MX10-LP and MX10-SP. Compared with MRE16 virus, MRE16SP virus has a deletion of 30 aa in the E2 gene (200-229), which has been shown to be the molecular basis for the different plaque morphology. However, the MX10-SP virus did not have the 30-amino-acid deletion in the E2 gene. These results demonstrate that the molecular basis for the different plaque morphology of MX10 virus, the first strain of the O/A genotype of SINV isolated from China, is different from that of the prototype MRE16 virus.
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Metadata
Title
Phenotypic and molecular characteristics of plaque-purified MX10 virus, an Oriental-Australian genotype of Sindbis virus from Yunnan, China
Authors
Wang Jinglin
Zhu Wuyang
Shihong Fu
Li Wenjuan
Zhang Hailin
Guodong Liang
Publication date
01-01-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 1/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1464-y

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