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01-03-2013 | Immunology in Colorado

Phenotype and function of protective, CD4-independent CD8 T cell memory

Authors: Lindsay E. Edwards, Catherine Haluszczak, Ross M. Kedl

Published in: Immunologic Research | Issue 1-3/2013

Abstract

While the need for CD4 T cells in the generation of CD8 T cell memory has been well documented, the mechanism underlying their requirement remains unknown. Here, we detail an immunization method capable of generating CD8 memory T cells that are indifferent to CD4 T cell help. Using a subunit vaccination that combines polyIC and an agonistic CD40 antibody, we program protective CD4-independent CD8 T cell memory. When cells generated by combined polyIC/CD40 immunization are compared to cells produced following a CD4-dependent vaccination, Listeria monocytogenes, they display dramatic differences, both phenotypically and functionally. The memory cells generated in a CD4-deficient host by polyIC/CD40 immunization provide protection against secondary infectious challenge, whereas cells generated by LM immunization in the same environment do not. Interestingly, combined polyIC/CD40 immunization generates long-term memory cells with low Blimp-1 and elevated Eomes expression despite high expression of Blimp-1 during the primary response. The potency of combined polyIC/CD40 to elicit CD8+ T cell memory in the absence of CD4 T cells suggests that it could be considered as a vaccine adjuvant in clinical situations where CD4 responses/numbers are compromised.

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Title: Phenotype and function of protective, CD4-independent CD8 T cell memory
Authors: Lindsay E. Edwards
Catherine Haluszczak
Ross M. Kedl
Publication date: 01-03-2013
Publisher: Springer-Verlag
Published in: Immunologic Research / Issue 1-3/2013
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI: https://doi.org/10.1007/s12026-012-8356-9

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Phenotype and function of protective, CD4-independent CD8 T cell memory

Abstract

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