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01-02-2009 | Experimental Study

Peripheral nerve regeneration: autologous conduit of vein plus perineurium

Authors: G. Di Benedetto, L. Grassetti, R. Mazzucchelli, M. Scarpelli, A. Bertani

Published in: European Journal of Plastic Surgery | Issue 1/2009

Abstract

A new method to enhance peripheral nerve regeneration is described. A 2-cm-long segment of right sciatic nerve of 90 Sprague-Dawley rats was transected and the gap was then bridged using one of the three methods: group 1 (treated, n ₁ = 30), a 2-cm-long strip of perineurium inserted inside a 2-cm-long vein graft; group 2 (control, n ₂ = 30), a vein graft alone; group 3 (standard nerve graft, n ₃ = 30), a resected segment of the sciatic nerve. Clinical and electrophysiological tests and quantitative analysis carried out 12 weeks after surgical procedure showed a good nerve regeneration for the treated and the control vein group, although it was lower than the standard nerve graft group. G factor values, index of nervous myelinization, were not significantly different between groups 1 and 3 (P < 0.05), but either were significantly lower than that of group 2.

Dahlin LB, Lundborg G (2001) Use of tubes on peripheral nerve repair. Neurosurg Clin N Am 12:341–352PubMed

Heijke GC, Klopper PJ, Baljet B et al (2001) Silicone rubber tubulization in peripheral sensory nerve reconstruction: an experimental study in rabbits. Microsurgery 21:306–316. doi:10.1002/micr.1057 PubMedCrossRef

Itoh S, Takakuda K, Kawabata S et al (2002) Evaluation of cross-linking procedures of collagen tubes used in peripheral nerve repair. Biomaterials 23(23):4475–4481. doi:10.1016/S0142-9612(02)00166-7 PubMedCrossRef

Stang F, Fansa H, Wolf G et al (2005) Collagen nerve conduits-assessment of biocompatibility and axonal regeneration. Biomed Mater Eng 15:3–12PubMed

Stang F, Fansa H, Wolf G et al (2005) Structural parameters of collagen nerve grafts influence peripheral nerve regeneration. Biomaterials 26:3083–3091. doi:10.1016/j.biomaterials.2004.07.060 PubMedCrossRef

Fansa H, Schneider W, Wolf G et al (2002) Influence of insulin-like growth factor-I (IGF-I) on nerve autografts and tissue-engineered nerve grafts. Muscle Nerve 26(1):87–93. doi:10.1002/mus.10165 PubMedCrossRef

Zhang F, Blain B, Beck J et al (2002) Autogenous venous graft with one-stage prepared Schwann cells as a conduit for repair of long segmental nerve defects. J Reconstr Microsurg 18(4):295–300. doi:10.1055/s-2002-30186 PubMedCrossRef

Young RC, Wiberg M, Terenghi G (2002) Poly-3-hydroxybutyrate (PHB): a resorbable conduit for long gap repair in peripheral nerve. Br J Plast Surg 55(3):235–240. doi:10.1054/bjps.2002.3798 PubMedCrossRef

Mligiliche N, Tabata Y, Endoh K et al (2001) Peripheral nerve regeneration through a long detergent-denatured muscle autografts in rabbits. Neuroreport 12(8):1719–1722. doi:10.1097/00001756-200106130-00040 PubMedCrossRef

10.

Di Benedetto G, Zura G, Mazzucchelli R et al (1998) Nerve regeneration through a combined autologous conduit (vein plus acellular muscle grafts). Biomaterials 19:173–181. doi:10.1016/S0142-9612(97)00200-7 PubMedCrossRef

11.

Raimondo S, Nicolino S, Tos P et al (2005) Schwann cell behavior after nerve repair by means of tissue-engineered muscle-vein combined guides. J Comp Neurol 489:249–259. doi:10.1002/cne.20625 PubMedCrossRef

12.

Lundborg G, Dahlin LB, Danielsen NP et al (1981) Reorganization and orientation of regenerating nerve fibres, perineurium and epineurium in preformed mesothelial tubes—an experimental study on the sciatic nerve of rats. J Neurosci Res 6:265–283. doi:10.1002/jnr.490060302 PubMedCrossRef

13.

De Medinaceli L, Freed WJ, Wyatt RJ (1982) An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol 77:634–643. doi:10.1016/0014-4886(82)90234-5 PubMedCrossRef

14.

Chiu DTW, Janecka I, Krizek TJ et al (1982) Autogenous vein grafts as a conduit for nerve regeneration. Surgery 91:226–233PubMed

15.

Chiu DT, Strauch B (1990) A prospective clinical evaluation of autogenous vein grafts used as a nerve conduit for distal sensory nerve defects of 3 cm or less. Plast Reconstr Surg 86(5):928–934 NovPubMedCrossRef

16.

Suematsu N, Atsuta Y, Hirayama T (1988) Vein graft for repair of peripheral nerve gap. J Reconstr Microsurg 4:313–318PubMedCrossRef

17.

Wang K-K, Costas PD, Jones DS et al (1992) Sleeve insertion and collagen coating improve nerve regeneration through vein conduits. J Reconstr Microsurg 9:39–48CrossRef

18.

Chen YS, Hsieh CL, Tsai CC et al (2000) Peripheral nerve regeneration using silicone rubber chambers filled with collagen, laminin and fibronectin. Biomaterials 21:1541–1547. doi:10.1016/S0142-9612(00)00028-4 PubMedCrossRef

19.

Meek MF, Robinson PH, Stokroos I et al (2001) Electron microscopical evaluation of short-term nerve regeneration through a thin-walled biodegradable poly (DLLA-epsilon-CL) nerve guide filled with modified denatured muscle tissue. Biomaterials 22:1177–1185. doi:10.1016/S0142-9612(00)00340-9 PubMedCrossRef

20.

Wells MR, Kraus K, Batter DK et al (1997) Gel matrix vehicles for growth factors application in nerve gap injuries repaired with tubes: a comparison of biomatrix, collagen, and methylcellulose. Exp Neurol 149:395–402. doi:10.1006/exnr.1997.6543 CrossRef

21.

Santo Neto H, Teodori RM, Somazz MC et al (1998) Axonal regeneration through muscle autografts submitted to local anaesthetic pre-treatment. Br J Plast Surg 51(7):555–560PubMed

Title: Peripheral nerve regeneration: autologous conduit of vein plus perineurium
Authors: G. Di Benedetto
L. Grassetti
R. Mazzucchelli
M. Scarpelli
A. Bertani
Publication date: 01-02-2009
Publisher: Springer-Verlag
Published in: European Journal of Plastic Surgery / Issue 1/2009
Print ISSN: 0930-343X
Electronic ISSN: 1435-0130
DOI: https://doi.org/10.1007/s00238-008-0302-7

At a glance: The STEP trials

Springer Medicine

Peripheral nerve regeneration: autologous conduit of vein plus perineurium

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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