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Allergy, Asthma & Clinical Immunology

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Open Access 01-12-2014 | Short report

Peripheral blood T cells and neutrophils from asthma patients express class-I MHC-restricted T cell-associated molecule

Authors: Carlos Ramirez-Velazquez, Nonantzin Beristain-Covarrubias, Leopoldo Guido-Bayardo, Vianney Ortiz-Navarrete

Published in: Allergy, Asthma & Clinical Immunology | Issue 1/2014

Abstract

Background

Class-I MHC-restricted T cell-associated molecule (CRTAM) is a protein expressed by activated natural killer T (NKT) cells, natural killer (NK) cells, CD8 T cells, and certain CD4 T lymphocytes. It is also expressed in Purkinje neurons and epithelial cells. However, no studies have examined the expression of CRTAM in peripheral blood cells during homeostasis or disease. Therefore, we explored whether CRTAM expression is influenced by the presence of allergic asthma.

Methods

We collected whole peripheral blood cells from non-asthmatic control subjects (n = 17) and patients with asthma (n = 17). All patients with asthma tested positive in allergen skin prick tests. We analyzed CRTAM expression in CD4⁺ and CD8⁺ T lymphocyte populations. CRTAM expression was also analyzed in CD177⁺ neutrophils and IL5Rα⁺ eosinophils.

Findings

The percentage of CD4⁺CRTAM⁺ and CD8⁺CRTAM⁺T lymphocytes in peripheral blood was higher in allergic asthma patients compared with healthy controls. Furthermore, the percentage of CD177⁺CRTAM⁺ neutrophils in peripheral blood was also elevated in patients with allergic asthma. However, the percentage of IL5Rα⁺CRTAM⁺ eosinophils in peripheral blood was not significantly different in patients with allergic asthma compared with healthy controls.

Conclusions

CRTAM expression on T cells, eosinophils, and neutrophils may be involved in bronchial inflammation in allergic asthma. Determination of CRTAM expression in peripheral blood may be useful for the diagnosis of bronchial inflammation and/or to identify recently activated immune cells.

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Title: Peripheral blood T cells and neutrophils from asthma patients express class-I MHC-restricted T cell-associated molecule
Authors: Carlos Ramirez-Velazquez
Nonantzin Beristain-Covarrubias
Leopoldo Guido-Bayardo
Vianney Ortiz-Navarrete
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Allergy, Asthma & Clinical Immunology / Issue 1/2014
Electronic ISSN: 1710-1492
DOI: https://doi.org/10.1186/1710-1492-10-46

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Peripheral blood T cells and neutrophils from asthma patients express class-I MHC-restricted T cell-associated molecule

Abstract

Background

Methods

Findings

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Findings

Conclusions

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