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CardioVascular and Interventional Radiology

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01-12-2011 | Laboratory Investigation

Percutaneous Irreversible Electroporation Lung Ablation: Preliminary Results in a Porcine Model

Authors: Ajita Deodhar, Sébastien Monette, Gordon W. Single Jr, William C. Hamilton Jr, Raymond H. Thornton, Constantinos T. Sofocleous, Majid Maybody, Stephen B. Solomon

Published in: CardioVascular and Interventional Radiology | Issue 6/2011

Abstract

Objective

Irreversible electroporation (IRE) uses direct electrical pulses to create permanent “pores” in cell membranes to cause cell death. In contrast to conventional modalities, IRE has a nonthermal mechanism of action. Our objective was to study the histopathological and imaging features of IRE in normal swine lung.

Materials and Methods

Eleven female swine were studied for hyperacute (8 h), acute (24 h), subacute (96 h), and chronic (3 week) effects of IRE ablation in lung. Paired unipolar IRE applicators were placed under computed tomography (CT) guidance. Some applicators were deliberately positioned near bronchovascular structures. IRE pulse delivery was synchronized with the cardiac rhythm only when ablation was performed within 2 cm of the heart. Contrast-enhanced CT scan was performed immediately before and after IRE and at 1 and 3 weeks after IRE ablation. Representative tissue was stained with hematoxylin and eosin for histopathology.

Results

Twenty-five ablations were created: ten hyperacute, four acute, and three subacute ablations showed alveolar edema and necrosis with necrosis of bronchial, bronchiolar, and vascular epithelium. Bronchovascular architecture was maintained. Chronic ablations showed bronchiolitis obliterans and alveolar interstitial fibrosis. Immediate post-procedure CT images showed linear or patchy density along the applicator tract. At 1 week, there was consolidation that resolved partially or completely by 3 weeks. Pneumothorax requiring chest tube developed in two animals; no significant cardiac arrhythmias were noted.

Conclusion

Our preliminary porcine study demonstrates the nonthermal and extracellular matrix sparing mechanism of action of IRE. IRE is a potential alternative to thermal ablative modalities.

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Title: Percutaneous Irreversible Electroporation Lung Ablation: Preliminary Results in a Porcine Model
Authors: Ajita Deodhar
Sébastien Monette
Gordon W. Single Jr
William C. Hamilton Jr
Raymond H. Thornton
Constantinos T. Sofocleous
Majid Maybody
Stephen B. Solomon
Publication date: 01-12-2011
Publisher: Springer-Verlag
Published in: CardioVascular and Interventional Radiology / Issue 6/2011
Print ISSN: 0174-1551
Electronic ISSN: 1432-086X
DOI: https://doi.org/10.1007/s00270-011-0143-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Percutaneous Irreversible Electroporation Lung Ablation: Preliminary Results in a Porcine Model

Abstract

Objective

Materials and Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and Methods

Results

Conclusion

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