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01-10-2012 | Original Article

PCR-restriction endonuclease analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats, sheep, and cattle in Jordan

Authors: Nabil Hailat, Adnan Fayyad, Mustafa Ababneh, Wael Hananeh, Feth Eddine Rezig, Saied Jaradat

Published in: Comparative Clinical Pathology | Issue 5/2012

Abstract

Paratuberculosis is an endemic disease and induces high economical losses in Jordan. There is no information available on genotypic variation of Mycobacterium avium subsp. paratuberculosis (MAP) isolated from animals in Jordan. In this study, we investigated 150 fecal samples from sheep, goats, and cattle for the presence of paratuberculosis using bacterial culture and polymerase chain reaction (PCR)-RFLP analysis of insertion sequence IS1311. Analysis of the results revealed that genotypic information from sheep, goat, and cattle could classify them into cattle or sheep strains. All culture isolates from cattle, 12.5% of the isolates from sheep, and 50% of the isolates from goats were cattle strain, while 87.5% of the isolates from sheep and 50% of the isolates from goats were sheep strain. Sequencing of the IS1311 268 bp PCR product from the three animal species confirmed the different MAP patterns.

Bauerfeind R, Benazzi S, Weiss R, Schliesser T, Willems H, Baljer G (2006) Molecular characterization of Mycobacterium paratuberculosis isolates from sheep, goats, and cattle by hybridization with a DNA probe to insertion element IS900. J Clin Microbiol 34:1617–1621

Buergelt CD, Donovan GA, Williams JE (2004) Identification of Mycobacterium avium subspecies paratuberculosis by polymerase chain reaction in blood and semen of a bull with clinical paratuberculosis. Intern J Appl Res Vet Med 2:130–134

Bull TJ, Hermon-Taylor J, Pavlik, El-Zaatari F, Tizard M (2000) Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing. Microbiol 146:2185–2219

Collins DM, Gabric DM, De Lisle GW (1990) Identification of two groups of Mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization. J Clin Microbiol 28:1591–1596PubMed

Collins DM, Hilbink F, West DM, Hosie BD, Cooke MM, De lisle GW (1993) Investigation of Mycobacterium paratuberculosis in sheep by faecal culture, DNA characterization and the polymerase chain reaction. Vet Rec 133:599–600PubMed

Djønne B, Pavlik I, Svastov P, Bartos M, Holstad G (2005) IS900 restriction fragment length polymorphism (RFLP) analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats and cattle in Norway. Acta vet Scand 46:13–18PubMedCrossRef

Ellingson JLE, Bolin CA, Stabel JR (1998) Identification of a gene unique to Mycobacterium avium subspecies paratuberculosis and application to diagnosis of paratuberculosis. Mol Cell Probes 12:133–142PubMedCrossRef

Garrido JM, Cortabarria N, Oguiza JA, Aduriz G, Juste RA (2000) Use of a PCR method on fecal samples for diagnosis of sheep paratuberculosis. Vet Microbiol 77:379–386PubMedCrossRef

Hailat N, Hananeh W, Metekia AS, Stabel JR, Al-Majali A, Lafi S (2010) Pathology of subclinical paratuberculosis (Johne’s disease) in Awassi sheep with reference to its occurrence in Jordan. Vetrinari Medicina 55:550–602

Lilenbaum W, Marassi CD, Oelemann WMR (2007) Paratuberculosis: an update. Braz J Microbiol 38:580–590CrossRef

Marsh I, Whittington R, Cousins D (1999) PCR-restriction endonuclease analysis for identification and strain typing of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium based on polymorphisms in IS1311. Mol Cell Probes 13:115–126PubMedCrossRef

Motiwala AS, Amonsin A, Strother M, Manning JB, Kapur V, Sreevatsan S (2004) Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis isolates recovered from wild animal species. J Clin Microbiol 42:1703–1712PubMedCrossRef

Pavlik I, Bejckova L, Pavlas M, Rozsypalova Z, Koskova S (1995) Characterization by restriction endonuclease analysis and DNA hybridization using IS900 of bovine, ovine, caprine and human dependent strains of Mycobacterium paratuberculosis isolated in various localities. Vet Microbiol 45:311–318PubMedCrossRef

Sevilla I, Singh SV, Garrido JM, Aduriz G, Rodriguez S, Geijo MV, Whittington RJ, Saunders V, Whitlock RH, Juste RA (2005) Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regions. Rev Sci Tech 24:1061–1066PubMed

Sevilla I, Garrido JM, Geijo M, Juste RA (2007) Pulsed-field gel electrophoresis profile homogeneity of Mycobacterium avium subsp. paratuberculosis isolates from cattle and heterogeneity of those from sheep and goats. BMC Microbiology 7:18–33PubMedCrossRef

Sivakumar P, Tripathi BN, Singh N, Sharma AK (2006) Pathology of naturally occurring paratuberculosis in water buffaloes (Bubalus bubalis). Vet Pathol 43:455–462PubMedCrossRef

Stabel JR (1997) An improved method for cultivation of Mycobacterium paratuberculosis from bovine fecal samples and comparison to three other methods. J Vet Diagn Invest 9:375–380PubMedCrossRef

Whittington RJ, Taragel CA, Ottaway S, Marsh I, Seaman J, Fridriksdottir V (2001) Molecular epidemiological confirmation and circumstances of occurrence of sheep (S) strains of Mycobacterium avium subsp. paratuberculosis in cases of paratuberculosis in cattle in Australia and sheep and cattle in Iceland. Vet Microbiol 79:311–322PubMedCrossRef

Yadav D, Singh SV, Singh AV, Sevilla I, Juste RA, Singh PK, Sohal I (2007) Pathogenic ‘Bison-type’ Mycobacterium avium subsp. paratuberculosis genotype characterized from riverine buffalo (Bubalus bubalis) in North India. Comparat Immunol Microbiol Infect 78:34–37

Title: PCR-restriction endonuclease analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats, sheep, and cattle in Jordan
Authors: Nabil Hailat
Adnan Fayyad
Mustafa Ababneh
Wael Hananeh
Feth Eddine Rezig
Saied Jaradat
Publication date: 01-10-2012
Publisher: Springer-Verlag
Published in: Comparative Clinical Pathology / Issue 5/2012
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-010-1170-7

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PCR-restriction endonuclease analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats, sheep, and cattle in Jordan

Abstract

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Abstract

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