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Cancer Cell International
Published in: Cancer Cell International 1/2015

Open Access 01-12-2015 | Primary research

Pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells

Authors: Feng Jin, Ran Zhang, Song Feng, Chuan-Tao Yuan, Ren-Ya Zhang, Guang-Kui Han, Gen-Hua Li, Xi-Zhen Yu, Yang Liu, Ling-Sheng Kong, Shu-Ling Zhang, Lei Zhao

Published in: Cancer Cell International | Issue 1/2015

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Abstract

Background

This study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.

Methods

CD133 positive TJ905 glioma cells were separated by immunomagnetic beads to isolate glioma stem-like cells. TJ905 cells and stem-like cells were inoculated subcutaneously into the mice to establish model of transplanted tumor, respectively. Mice growing condition and behavior were observed. HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.

Results

The growing condition and behavior of mice in TJ905 stem cell group was more exaggerated and the models showed stronger malignant features pathologically than that in TJ905 cell group. Glial fibrillary acidic protein (GFAP) in TJ905 cell and stem-like cell group showed the transplanted tumor originated from astrocytes. Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.

Conclusions

Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant. Use of TJ905 stem cells to establish transplanted tumor model in nude mice is excellent for glioma research.
Literature
1.
Nieto-Sampedro M, Valle-Argos B, Gómez-Nicola D, Fernández-Mayoralas A, Nieto-Díaz M. Inhibitors of glioma growth that reveal the tumour to the immune system. Clin Med Insights Oncol. 2011;5:265–314.PubMedCentralPubMedCrossRef
2.
Tafani M, Di Vito M, Frati A, Pellegrini L, De Santis E, Sette G, et al. Pro-inflammatory gene expression in solid glioblastoma microenvironment and in hypoxic stem cells from human glioblastoma. J Neuroinflammation. 2011;8:32.PubMedCentralPubMedCrossRef
3.
Anton K, Baehring JM, Mayer T. Glioblastoma multiforme: overview of current treatment and future perspectives. Hematol Oncol Clin North Am. 2012;26(4):825–53.PubMedCrossRef
4.
5.
Cho DY, Lin SZ, Yang WK, Lee HC, Hsu DM, Lin HL, et al. Targeting cancer stem cells for treatment of glioblastoma multiforme. Cell Transplant. 2013;22(4):731–9.PubMedCrossRef
6.
Choy W, Nagasawa DT, Trang A, Thill K, Spasic M, Yang I. CD133 as a marker for regulation and potential for targeted therapies in glioblastoma multiforme. Neurosurg Clin N Am. 2012;23(3):391–405.PubMedCrossRef
7.
Ernst A, Hofmann S, Ahmadi R, Becker N, Korshunov A, Engel F, et al. Genomic and expression profiling of glioblastoma stem cell-like spheroid cultures identifies novel tumor-relevant genes associated with survival. Clin Cancer Res. 2009;15(21):6541–50.PubMedCrossRef
8.
Jin F, Gao C, Zhao L, Zhang H, Wang HT, Shao T, et al. Using CD133 positive U251 glioblastoma stem cells to establish nude mice model of transplanted tumor. Brain Res. 2011;1368:82–90.PubMedCrossRef
9.
Zhang A, Hao J, Wang K, Huang Q, Yu K, Kang C, et al. Down-regulation of miR-106b suppresses the growth of human glioma cells. J Neurooncol. 2013;112(2):179–89.PubMedCrossRef
10.
Jin F, Zhao L, Guo YJ, Zhao WJ, Zhang H, Wang HT, et al. Influence of Etoposide on anti-apoptotic and multidrug resistance-associated protein genes in CD133 positive U251 glioblastoma stem-like cells. Brain Res. 2010;1336:103–11.PubMedCrossRef
11.
Jin F, Cheng D, Tao JY, Zhang SL, Pang R, Guo YJ, et al. Anti-inflammatory and anti-oxidative effects of corilagin in a rat model of acute cholestasis. BMC Gastroenterol. 2013;13:79.PubMedCentralPubMedCrossRef
12.
Ahmadloo N, Kani AA, Mohammadianpanah M, Nasrolahi H, Omidvari S, Mosalaei A, et al. Treatment outcome and prognostic factors of adult glioblastoma multiforme. J Egypt Natl Canc Inst. 2013;25(1):21–30.PubMedCrossRef
13.
Zhang X, Zhang W, Mao XG, Zhen HN, Cao WD, Hu SJ. Targeting role of glioma stem cells for glioblastoma multiforme. Curr Med Chem. 2013;20(15):1974–84.PubMedCrossRef
14.
Sampetrean O, Saga I, Nakanishi M, Sugihara E, Fukaya R, Onishi N, et al. Invasion precedes tumor mass formation in a malignant brain tumor model of genetically modified neural stem cells. Neoplasia. 2011;13(9):784–91.PubMedCentralPubMedCrossRef
15.
Tunici P, Irvin D, Liu G, Yuan X, Zhaohui Z, Ng H, et al. Brain tumor stem cells: new targets for clinical treatments? Neurosurg Focus. 2006;20(4):E27.PubMedCrossRef
16.
17.
Jakobsen JN, Sørensen JB. Clinical impact of ki-67 labeling index in non-small cell lung cancer. Lung Cancer. 2013;79(1):1–7.PubMedCrossRef
18.
Luporsi E, André F, Spyratos F, Martin PM, Jacquemier J, Penault-Llorca F, et al. Ki-67: level of evidence and methodological considerations for its role in the clinical management of breast cancer: analytical and critical review. Breast Cancer Res Treat. 2012;132(3):895–915.PubMedCentralPubMedCrossRef
19.
Dougherty JD, Fomchenko EI, Akuffo AA, Schmidt E, Helmy KY, Bazzoli E, et al. Candidate pathways for promoting differentiation or quiescence of oligodendrocyte progenitor-like cells in glioma. Cancer Res. 2012;72(18):4856–68.PubMedCentralPubMedCrossRef
20.
Sica G, Lama G, Anile C, Geloso MC, La Torre G, De Bonis P, et al. Assessment of angiogenesis by CD105 and nestin expression in peritumor tissue of glioblastoma. Int J Oncol. 2011;38(1):41–9.PubMed
21.
Jin F, Zhao L, Zhao HY, Guo SG, Feng J, Jiang XB, et al. Comparison between cells and cancer stem-like cells isolated from glioblastoma and astrocytoma on expression of anti-apoptotic and multidrug resistance-associated protein genes. Neuroscience. 2008;154(2):541–50.PubMedCrossRef
22.
Jin F, Zhao L, Zhao HY, Guo SG, Feng J, Jiang XB, et al. Paradoxical expression of anti-apoptotic and MRP genes on cancer stem-like cell isolated from TJ905 glioblastoma multiforme cell line. Cancer Invest. 2008;26(4):338–43.PubMedCrossRef
Metadata
Title
Pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells
Authors
Feng Jin
Ran Zhang
Song Feng
Chuan-Tao Yuan
Ren-Ya Zhang
Guang-Kui Han
Gen-Hua Li
Xi-Zhen Yu
Yang Liu
Ling-Sheng Kong
Shu-Ling Zhang
Lei Zhao
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2015
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-015-0208-y

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