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01-02-2016 | Original Article

Partial MHC Constructs Treat Thromboembolic Ischemic Stroke Characterized by Early Immune Expansion

Authors: Abby L. Dotson, Yingxin Chen, Wenbin Zhu, Nicole Libal, Nabil J. Alkayed, Halina Offner

Published in: Translational Stroke Research | Issue 1/2016

Abstract

Inflammation and thrombosis are tightly linked, with inflammation contributing to thromboembolism and to stroke outcome. Thromboembolism is a frequent cause of ischemic stroke; yet, the most used occlusion mouse models of experimental stroke do not effectively replicate thromboembolism. Our group recently described a novel thromboembolic mouse model of stroke that successfully occludes the middle cerebral artery with high reproducibility. In the current study, we characterize the peripheral and local immune outcomes as well as the ischemic response to immune therapy in a clinically relevant mouse model of thromboembolic stroke. Brain and spleen tissues were harvested 24 h after thromboembolic stroke and cells immunophenotyped by flow cytometry. We observed a significant increase in neutrophils and early activated T cells in the spleen and an increase in neutrophils and activated monocytes/microglia in the ischemic cortex after thromboembolic stroke. Moreover, as was shown previously for transient MCAO models, treatment of thromboembolic stroke with partial MHC constructs significantly reduced ischemic damage indicating an equivalent effect of this immune-based therapy in the thromboembolic model that better mimics the pathophysiology of human stroke.

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Title: Partial MHC Constructs Treat Thromboembolic Ischemic Stroke Characterized by Early Immune Expansion
Authors: Abby L. Dotson
Yingxin Chen
Wenbin Zhu
Nicole Libal
Nabil J. Alkayed
Halina Offner
Publication date: 01-02-2016
Publisher: Springer US
Published in: Translational Stroke Research / Issue 1/2016
Print ISSN: 1868-4483
Electronic ISSN: 1868-601X
DOI: https://doi.org/10.1007/s12975-015-0436-4

At a glance: The STEP trials

Springer Medicine

Partial MHC Constructs Treat Thromboembolic Ischemic Stroke Characterized by Early Immune Expansion

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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