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01-04-2020 | Parkinson's Disease | Original Article

MicroRNA-216a inhibits neuronal apoptosis in a cellular Parkinson’s disease model by targeting Bax

Authors: Xiaobo Yang, Meng Zhang, Meng Wei, Anqi Wang, Yongning Deng, Hongmei Cao

Published in: Metabolic Brain Disease | Issue 4/2020

Abstract

The study found that microRNAs play an important role in Parkinson’s disease (PD). However, the function of MicroRNA-216a (miR-216a) in PD is unclear. Therefore, this experiment aimed to investigate the pathogenesis of miR-216a in PD. Using the toxicity of MPP+ to polyhexamine neurons, apoptosis of SH-SY5Y neuroblastoma cells was induced at different time by MPP+ to construct a stable acute PD cell model. The effects of DNA breakage, mitochondrial membrane potential (A ^ m), caspase-3 activity and nucleosome enrichment on cell apoptosis were detected by flow cytometry, TUNEL. MPP+ increased the toxic effects of dopaminergic neurons in a PD model. The introduction of miR-216a inhibited MPP + -induced neuronal apoptosis. The main manifestations were the decreased levels of positive rate of Tunel cells, caspase 3 activity and nucleosome enrichment factor. Bax was a direct target of miR-216a. In addition, Bax overexpression reversed the effects of miR-216a on neural cells. Bax downstream factors were also involved in miR-216a regulation of MPP + -triggered neuronal apoptosis. miR-216a regulated the progression of PD by regulating Bax, and miR-216a may be a potential target for PD.

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Title: MicroRNA-216a inhibits neuronal apoptosis in a cellular Parkinson’s disease model by targeting Bax
Authors: Xiaobo Yang
Meng Zhang
Meng Wei
Anqi Wang
Yongning Deng
Hongmei Cao
Publication date: 01-04-2020
Publisher: Springer US
Keyword: Parkinson's Disease
Published in: Metabolic Brain Disease / Issue 4/2020
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-020-00546-x

At a glance: The STEP trials

Springer Medicine

MicroRNA-216a inhibits neuronal apoptosis in a cellular Parkinson’s disease model by targeting Bax

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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